{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:12:48Z","timestamp":1787004768585,"version":"build-2736575974"},"reference-count":37,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T00:00:00Z","timestamp":1657065600000},"content-version":"vor","delay-in-days":186,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2019YFC1605700"],"award-info":[{"award-number":["2019YFC1605700"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32102689"],"award-info":[{"award-number":["32102689"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2022,6,24]]},"published-print":{"date-parts":[[2022]]},"abstract":"<jats:p>\n                    <jats:italic>Giardia duodenalis<\/jats:italic>\n                    is an important zoonotic parasite that causes economic losses to animal husbandry and threatens public health. In the present study, a total of 1466 fresh fecal samples were collected from sheep (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a0797), goats (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a0561) and beef cattle (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a0108) in Southwest Inner Mongolia, China.\n                    <jats:italic>Giardia duodenalis<\/jats:italic>\n                    was initially screened via nested polymerase chain reaction (PCR) targeting the \u03b2-giardin (\n                    <jats:italic>bg<\/jats:italic>\n                    ) gene, and\n                    <jats:italic>bg<\/jats:italic>\n                    -positive samples were subjected to PCR amplification targeting the glutamate dehydrogenase (\n                    <jats:italic>gdh<\/jats:italic>\n                    ) and triose phosphate isomerase (\n                    <jats:italic>tpi<\/jats:italic>\n                    ) genes. A total of 4.0% of samples (58\/1466) were positive for\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    , with a prevalence of 3.4% in sheep, 3.7% in goats and 5.2% in beef cattle. Three\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    assemblages (A, B, and E) were identified, with E as the prevalent assemblage. Four and one novel assemblage E sequences were obtained for the\n                    <jats:italic>gdh<\/jats:italic>\n                    and\n                    <jats:italic>tpi<\/jats:italic>\n                    loci, respectively and four assemblage E multilocus genotypes (MLG) were obtained. This study demonstrates high genetic variations in\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    assemblage E, and provides baseline data for preventing and controlling\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    infection in livestock in Inner Mongolia.\n                  <\/jats:p>","DOI":"10.1051\/parasite\/2022036","type":"journal-article","created":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T03:52:56Z","timestamp":1657252376000},"page":"33","source":"Crossref","is-referenced-by-count":20,"title":["Molecular characterizations of\n                    <i>Giardia duodenalis<\/i>\n                    based on multilocus genotyping in sheep, goats, and beef cattle in Southwest Inner Mongolia, China"],"prefix":"10.1051","volume":"29","author":[{"given":"Yin","family":"Fu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heping","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaokun","family":"Bian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyang","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiashu","family":"Lang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junchen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5706-131X","authenticated-orcid":false,"given":"Longxian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2022,7,6]]},"reference":[{"issue":"1\u20132","key":"R1","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.vetpar.2006.06.023","volume":"142","author":"Aloisio","year":"2006","journal-title":"Veterinary Parasitology"},{"issue":"4","key":"R2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/S0304-4017(02)00422-3","volume":"112","author":"Appelbee","year":"2003","journal-title":"Canada. 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