{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T18:32:45Z","timestamp":1792521165451,"version":"4.5.0"},"reference-count":37,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T00:00:00Z","timestamp":1665446400000},"content-version":"vor","delay-in-days":283,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172882, U1904203"],"award-info":[{"award-number":["32172882, U1904203"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Key Program of the National Natural Science Foundation of China","award":["31330079"],"award-info":[{"award-number":["31330079"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2022,9,17]]},"published-print":{"date-parts":[[2022]]},"abstract":"<jats:p><jats:italic>Cryptosporidium<\/jats:italic> spp., <jats:italic>Enterocytozoon bieneusi<\/jats:italic> and <jats:italic>Giardia duodenalis<\/jats:italic> are significant zoonotic intestinal pathogens that can cause gastrointestinal symptoms such as diarrhea and induce a host immune response. A total of 1237 fecal samples were collected from laboratory rodents (rats, mice and guinea pigs) from four different locations in China to investigate the infection rates and molecular characterization of these pathogens on experimental animals. Genomic DNA was extracted from each sample, and PCR amplifications were done. Overall, the <jats:italic>Cryptosporidium<\/jats:italic> spp. infection rate was 3.8% (47\/1237). Four known <jats:italic>Cryptosporidium<\/jats:italic> species were identified, namely <jats:italic>C. parvum<\/jats:italic>, <jats:italic>C. muris<\/jats:italic>, <jats:italic>C<\/jats:italic>. <jats:italic>tyzzeri<\/jats:italic> and <jats:italic>C. homai<\/jats:italic>, the three former being zoonotic species. The overall <jats:italic>E. bieneusi<\/jats:italic> infection rate was 3.0% (37\/1237). Seven known <jats:italic>E. bieneusi<\/jats:italic> genotypes, namely S7, BEB6, J, Henan-IV, CHG10, D and WL6, were detected by sequence analysis. Among these, genotypes D, Henan-IV and CHG10 have a high zoonotic risk. <jats:italic>Giardia duodenalis<\/jats:italic> was not detected at any of the three loci (SSU rRNA, <jats:italic>bg<\/jats:italic> and <jats:italic>gdh<\/jats:italic>) after PCR amplification. This study provides basic data for these pathogens in laboratory rodents in China and lays the foundation for their prevention and control in laboratory animals.<\/jats:p>","DOI":"10.1051\/parasite\/2022046","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T08:09:02Z","timestamp":1665475742000},"page":"46","source":"Crossref","is-referenced-by-count":17,"title":["Molecular characterization of <i>Cryptosporidium<\/i> spp., <i>Enterocytozoon bieneusi<\/i> and <i>Giardia duodenalis<\/i> in laboratory rodents in China"],"prefix":"10.1051","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8269-7010","authenticated-orcid":false,"given":"Nanhao","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufeng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfeng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5706-131X","authenticated-orcid":false,"given":"Longxian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2022,10,11]]},"reference":[{"issue":"1","key":"R1","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1007\/s12639-021-01420-y","volume":"46","author":"Akanbi","year":"2022","journal-title":"Journal of Parasitic Diseases"},{"issue":"1","key":"R2","first-page":"20","volume":"21","author":"Almugadam","year":"2021","journal-title":"Infectious Diseases"},{"issue":"4","key":"R3","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/S0304-4017(02)00422-3","volume":"112","author":"Appelbee","year":"2003","journal-title":"Veterinary Parasitology"},{"issue":"2","key":"R4","first-page":"105","volume":"63","author":"Ayinmode","year":"2017","journal-title":"Annals of Parasitology"},{"issue":"2","key":"R5","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1128\/CMR.11.2.231","volume":"11","author":"Baker","year":"1998","journal-title":"Clinical Microbiology Reviews"},{"issue":"3","key":"R6","first-page":"910","volume":"104","author":"Belkessa","year":"2021","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"issue":"5","key":"R7","doi-asserted-by":"crossref","first-page":"2595","DOI":"10.1128\/AEM.68.5.2595-2599.2002","volume":"68","author":"Buckholt","year":"2002","journal-title":"Applied and Environmental Microbiology"},{"issue":"13","key":"R8","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1016\/j.ijpara.2008.04.008","volume":"38","author":"Cacci\u00f2","year":"2008","journal-title":"International Journal for Parasitology"},{"issue":"8","key":"R9","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.1128\/JCM.00725-07","volume":"45","author":"Cama","year":"2007","journal-title":"Journal of Clinical Microbiology"},{"key":"R10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ejop.2017.12.006","volume":"63","author":"\u010condlov\u00e1","year":"2018","journal-title":"European Journal of Protistology"},{"issue":"1","key":"R11","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/s13071-020-04566-5","volume":"14","author":"Fan","year":"2021","journal-title":"Parasites & Vectors"},{"issue":"1","key":"R12","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1128\/AEM.02997-13","volume":"80","author":"Guo","year":"2014","journal-title":"Applied and Environmental Microbiology"},{"issue":"6","key":"R13","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1007\/s00436-016-4967-8","volume":"115","author":"Holubov\u00e1","year":"2016","journal-title":"Parasitology Research"},{"key":"R14","doi-asserted-by":"crossref","unstructured":"Karimi K, Mirjalali H, Niyyati M, Haghighi A, Pourhoseingholi MA, Sharifdini M, Naderi N, Zali MR. 2020. 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