{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:53:48Z","timestamp":1787165628796,"version":"build-2736575974"},"reference-count":34,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T00:00:00Z","timestamp":1667952000000},"content-version":"vor","delay-in-days":312,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2022,10,6]]},"published-print":{"date-parts":[[2022]]},"abstract":"<jats:p>\n                    <jats:italic>Background<\/jats:italic>\n                    : IgG detection to determine immune status to\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    infection and seroconversion mainly relies on ELISA techniques and, if necessary, on a confirmatory test, Western blot. This study evaluated the performance of the\n                    <jats:italic>recom<\/jats:italic>\n                    Line\n                    <jats:italic>Toxoplasma<\/jats:italic>\n                    IgG immunoblot (IB-\n                    <jats:italic>recom<\/jats:italic>\n                    Line) (Mikrogen) as a confirmatory test on a large number of sera. A total of 171 sera were selected (113 patients) and had previously been analyzed by two ELISA tests, ARCHITECT (Abbott) and VIDAS (bioM\u00e9rieux)\u00a0\u00b1\u00a0LDBIO-Toxo II IgG Western blot (WB-LDBIO) (LDBio). The sera were classified into three groups: group 1 included 50 sera without difficulty in interpreting the IgG results (patients with documented past infection or uninfected); group 2 included 47 sera with difficulty in interpreting the ELISA results; and group 3 included 74 sequential sera from 25 pregnant women with seroconversion.\n                    <jats:italic>Results<\/jats:italic>\n                    : In group 1, overall IgG agreements were 94% and 90% with ARCHITECT and VIDAS, respectively. In group 2, low agreement was observed between IB-\n                    <jats:italic>recom<\/jats:italic>\n                    Line and WB-LDBIO, with eight false-positive and 13 false-negative results. In group 3, 4\/13 seroconversions were detected earlier with IB-\n                    <jats:italic>recom<\/jats:italic>\n                    Line compared to other tests.\n                    <jats:italic>Conclusions<\/jats:italic>\n                    : IB-\n                    <jats:italic>recom<\/jats:italic>\n                    Line allowed for earlier diagnosis of toxoplasmic seroconversion compared to both ELISA tests and WB-LDBIO but led to insufficient performance to confirm the immune status when ELISA results were discordant or equivocal.\n                  <\/jats:p>","DOI":"10.1051\/parasite\/2022050","type":"journal-article","created":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T04:05:26Z","timestamp":1667966726000},"page":"52","source":"Crossref","is-referenced-by-count":2,"title":["Serological diagnosis of toxoplasmosis: evaluation of the commercial test\n                    <i>recom<\/i>\n                    Line\n                    <i>Toxoplasma<\/i>\n                    IgG immunoblot (Mikrogen) based on recombinant antigens"],"prefix":"10.1051","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0433-0141","authenticated-orcid":false,"given":"Vincent","family":"Jean-Pierre","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Miozzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Fricker-Hidalgo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Garnaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie Gladys","family":"Robert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Pelloux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Pierre","family":"Brenier-Pinchart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2022,11,9]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.diagmicrobio.2016.10.020","volume":"87","author":"Armengol","year":"2017","journal-title":"Diagnostic Microbiology and Infectious Disease"},{"key":"R2","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1128\/JCM.38.3.1144-1150.2000","volume":"38","author":"Aubert","year":"2000","journal-title":"Journal of Clinical Microbiology"},{"key":"R3","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.mimet.2017.01.003","volume":"134","author":"Dard","year":"2017","journal-title":"Journal of Microbiological Methods"},{"key":"R4","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.pt.2016.04.001","volume":"32","author":"Dard","year":"2016","journal-title":"Trends in Parasitology"},{"key":"R5","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.pt.2020.09.006","volume":"37","author":"Dupont","year":"2021","journal-title":"Trends in Parasitology"},{"key":"R6","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1128\/JCM.00182-08","volume":"46","author":"Franck","year":"2008","journal-title":"Journal of Clinical Microbiology"},{"key":"R7","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1128\/JCM.00169-13","volume":"51","author":"Fricker-Hidalgo","year":"2013","journal-title":"Journal of Clinical Microbiology"},{"key":"R8","doi-asserted-by":"crossref","unstructured":"Fricker-Hidalgo H, Bailly S, Brenier-Pinchart M-P, Dard C, Jean D, Coston A-L, Garnaud C, Pelloux H. 2020. 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