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The aim of this study was to evaluate interactions between <jats:italic>C. parvum<\/jats:italic> oocysts and two common FLAs (<jats:italic>Acanthamoeba castellanii<\/jats:italic> and <jats:italic>Vermamoeba vermiformis)<\/jats:italic> in a water environment. Encystment and survival of FLAs were evaluated by microscopy using trypan blue vital coloration. Oocysts were numerated on microscopy. Interactions were studied over time in conditions both unfavorable and favorable to phagocytosis. Potential phagocytosis was directly evaluated by several microscopic approaches and indirectly by numeration of microorganisms and oocyst infectivity evaluation. Occasional phagocytosis of <jats:italic>C. parvum<\/jats:italic> by FLAs was documented. However, oocyst concentrations did not decrease significantly, suggesting resistance of oocysts to phagocytosis. A temporary decrease of oocyst infectivity was observed in the presence of <jats:italic>A. castellanii<\/jats:italic>. The effect of these interactions on <jats:italic>C. parvum<\/jats:italic> infectivity is particularly interesting. The biofilm condition could favor the persistence or even the proliferation of oocysts over time. This study demonstrated interactions between <jats:italic>C. parvum<\/jats:italic> and FLAs. Further knowledge of the mechanisms involved in the decrease of oocyst infectivity in the presence of <jats:italic>A. castellanii<\/jats:italic> could facilitate the development of new therapeutic approaches.<\/jats:p>","DOI":"10.1051\/parasite\/2023033","type":"journal-article","created":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T08:08:06Z","timestamp":1692691686000},"page":"31","source":"Crossref","is-referenced-by-count":1,"title":["Interactions between free-living amoebae and <i>Cryptosporidium parvum<\/i>: an experimental 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