{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:11:19Z","timestamp":1787497879195,"version":"build-2736575974"},"reference-count":36,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T00:00:00Z","timestamp":1698883200000},"content-version":"vor","delay-in-days":305,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Department of Education Scientific Research Project of Zhejiang","award":["Y202249687"],"award-info":[{"award-number":["Y202249687"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2023,10,19]]},"published-print":{"date-parts":[[2023]]},"abstract":"<jats:p>\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. is an important gastrointestinal parasite with global distribution, prevalent in humans, farmed animals, and wildlife. Therefore, this study aimed to investigate the prevalence and genetic diversity of\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. in Asiatic brush-tailed porcupines (\n                    <jats:italic>Atherurus macrourus<\/jats:italic>\n                    ), bamboo rats (\n                    <jats:italic>Rhizomys pruinosus<\/jats:italic>\n                    ), and masked palm civets (\n                    <jats:italic>Paguma larvata<\/jats:italic>\n                    ) in Hainan Province, China. A total of 900 fecal samples were collected from three farmed animal species including 257 porcupines, 360 rats, and 283 civets. Genomic DNA was extracted from each fecal sample and\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. was detected by PCR at the small subunit ribosomal RNA (\n                    <jats:italic>SSU<\/jats:italic>\n                    rRNA) gene. A phylogenetic tree was constructed using the maximum likelihood method.\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. was detected in 47 (5.2%) fecal samples: 12 (4.7%) Asiatic brush-tailed porcupines, 8 (2.2%) bamboo rats, and 27 (9.5%) masked palm civets. Three known\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. subtypes, including ST1, ST4, ST5, and one unnamed subtype (unST), were found in one, 19, 26, and one animal, respectively. Subtypes ST4 and unST were detected in porcupines, ST4 in rats, and ST1 and ST5 in civets. Our results suggest that the three farmed animal species reported in this study could serve as reservoirs for potentially zoonotic\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. subtypes and transmit this parasite to humans, other farmed animals, and wildlife.\n                  <\/jats:p>","DOI":"10.1051\/parasite\/2023048","type":"journal-article","created":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T04:59:33Z","timestamp":1698987573000},"page":"45","source":"Crossref","is-referenced-by-count":10,"title":["Prevalence and distribution of subtypes of\n                    <i>Blastocystis<\/i>\n                    in Asiatic brush-tailed porcupines (\n                    <i>Atherurus macrourus<\/i>\n                    ), bamboo rats (\n                    <i>Rhizomys pruinosus<\/i>\n                    ), and masked palm civets (\n                    <i>Paguma larvata<\/i>\n                    ) farmed in Hainan, China"],"prefix":"10.1051","volume":"30","author":[{"given":"Wei","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangxu","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Qiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuyi","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuankun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huicong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenting","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2023,11,2]]},"reference":[{"issue":"5","key":"R1","doi-asserted-by":"crossref","first-page":"125679","DOI":"10.1016\/j.protis.2019.125679","volume":"170","author":"AbuOdeh","year":"2019","journal-title":"Protist"},{"issue":"43","key":"R2","doi-asserted-by":"crossref","first-page":"60308","DOI":"10.1007\/s11356-021-16187-1","volume":"28","author":"Asghari","year":"2021","journal-title":"Environmental 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