{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T12:47:03Z","timestamp":1788439623467,"version":"build-2803163510"},"reference-count":50,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2023,12,13]],"date-time":"2023-12-13T00:00:00Z","timestamp":1702425600000},"content-version":"vor","delay-in-days":346,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"The Fundamental Fund of Khon Kaen University from National Science, Research and Innovation Fund, Thailand"},{"name":"Faculty of Medicine, Khon Kaen University","award":["IN64258"],"award-info":[{"award-number":["IN64258"]}]},{"name":"Faculty of Medicine, Khon Kaen University","award":["IN64258"],"award-info":[{"award-number":["IN64258"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2023,11,25]]},"published-print":{"date-parts":[[2023]]},"abstract":"<jats:p>Strongyloidiasis is a neglected tropical disease that can cause fatal complications due to hyperinfection and disseminated strongyloidiasis in immunocompromised patients. We used two <jats:italic>Strongyloides stercoralis<\/jats:italic> recombinant antigenic proteins, L3NieAg.01 (NIE) and IgG-immunoreactive antigen (SsIR), to develop the recombinant antigen-based immunochromatography test (ICT) kit. We constructed and compared kits using either the NIE (NIE ICT kit) or the SsIR (SsIR ICT kit) antigens and a kit using a mixture of both (NIE-SsIR ICT kit) for detection of anti-<jats:italic>Strongyloides<\/jats:italic> IgG antibody in human serum samples. Serum samples from normal healthy individuals (Group I, <jats:italic>n<\/jats:italic>\u00a0=\u00a040), proven strongyloidiasis patients (Group II, <jats:italic>n<\/jats:italic>\u00a0=\u00a0100), and those with other parasitic infections (Group III, <jats:italic>n<\/jats:italic>\u00a0=\u00a0154) were evaluated. Sensitivity and specificity were 81.0% and 84.0% for the NIE ICT kit, 89.0% and 83.5% for the SsIR ICT kit, and 95.0% and 90.2% for the NIE-SsIR ICT kit, respectively. The NIE-SsIR ICT kit provided the best diagnostic results; it can supplement stool examination for clinical diagnosis and can be used to screen for asymptomatic <jats:italic>S. stercoralis<\/jats:italic> infection in people at risk in endemic areas. The NIE-SsIR ICT kit can also be used in large-scale sero-epidemiological investigations in endemic areas without the need for additional facilities or ancillary supplies.<\/jats:p>","DOI":"10.1051\/parasite\/2023063","type":"journal-article","created":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T09:24:55Z","timestamp":1702632295000},"page":"60","source":"Crossref","is-referenced-by-count":4,"title":["Improved diagnostic sensitivity of human strongyloidiasis using point-of-care mixed recombinant antigen-based 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