{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:05:53Z","timestamp":1787169953838,"version":"build-2736575974"},"reference-count":32,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T00:00:00Z","timestamp":1718928000000},"content-version":"vor","delay-in-days":172,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Hunan Provincial Natural Science Foundation Committee","award":["Number: 2022JJ50286"],"award-info":[{"award-number":["Number: 2022JJ50286"]}]},{"name":"the Basic scientific research project of Wenzhou","award":["(Y2023070)"],"award-info":[{"award-number":["(Y2023070)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2024,5,24]]},"published-print":{"date-parts":[[2024]]},"abstract":"<jats:p>\n                    Wild rodents are key carriers of various human pathogens, including\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    spp. Our study aimed to assess the prevalence and genetic characteristics of\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    among wild rodents in the Inner Mongolian Autonomous Region and Liaoning Province of China. From November 2023 to February 2024, 486 rodents were captured in these regions. Fresh feces were collected from the intestines of each rodent for the isolation of DNA and PCR amplification of the vertebrate cytochrome b (\n                    <jats:italic>cytb<\/jats:italic>\n                    ) gene to identify rodent species. Subsequently, PCR analysis and sequencing of the partial small subunit of the ribosomal RNA (\n                    <jats:italic>rRNA<\/jats:italic>\n                    ) gene were utilized to detect\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    in all fecal samples. Of the total samples, 27.4% (133\/486) were found to be\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    positive. The results revealed the presence of four species of rodents infected with\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    , 32.3% (63\/195) in\n                    <jats:italic>Rattus norvegicus<\/jats:italic>\n                    , 15.1% (16\/106) in\n                    <jats:italic>Mus musculus<\/jats:italic>\n                    , 20.2% (18\/89) in\n                    <jats:italic>Apodemus agrarius<\/jats:italic>\n                    , and 37.5% (36\/96) in\n                    <jats:italic>Cricetulus barabensis<\/jats:italic>\n                    . Sequence analysis confirmed the existence of five\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    subtypes: ST1 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a04), ST2 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a02), the ST4 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a0125, the dominant subtype), ST10 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a01), and a novel ST (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a01). The identified zoonotic subtypes (ST1, ST2, ST4, and ST10) highlight the possible role played by wild rodents in the transmission of\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    to humans, thereby elevating the chances of human infection. Meanwhile, the discovery of novel sequences also provides new insights into the genetic diversity of this parasite.\n                  <\/jats:p>","DOI":"10.1051\/parasite\/2024031","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T04:05:32Z","timestamp":1719201932000},"page":"33","source":"Crossref","is-referenced-by-count":8,"title":["Molecular investigation of\n                    <i>Blastocystis<\/i>\n                    sp. infections in wild rodents from the Inner Mongolian Autonomous Region and Liaoning province, China: High prevalence and dominance of ST4"],"prefix":"10.1051","volume":"31","author":[{"given":"Li","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fansheng","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2024,6,21]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"102124","DOI":"10.1016\/j.cimid.2024.102124","volume":"106","author":"Asghari","year":"2024","journal-title":"Comparative Immunology Microbiology and Infectious 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Molecular identification and genotyping of Blastocystis sp. in sheep and goats from some areas in Inner Mongolia, Northern China. 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