{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T05:01:56Z","timestamp":1781499716878,"version":"3.54.1"},"reference-count":40,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"vor","delay-in-days":182,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"the National Key Research and Development Program of China","award":["2021YFC2300902"],"award-info":[{"award-number":["2021YFC2300902"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["(82273693)"],"award-info":[{"award-number":["(82273693)"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Basic scientific research project of Wenzhou","award":["Y2023070"],"award-info":[{"award-number":["Y2023070"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2024,5,31]]},"published-print":{"date-parts":[[2024]]},"abstract":"<jats:p>Wild rodents serve as reservoirs for <jats:italic>Cryptosporidium<\/jats:italic> and are overpopulated globally. However, genetic data regarding <jats:italic>Cryptosporidium<\/jats:italic> in these animals from China are limited. Here, we have determined the prevalence and genetic characteristics of <jats:italic>Cryptosporidium<\/jats:italic> among 370 wild rodents captured from three distinct locations in the southern region of Zhejiang Province, China. Fresh feces were collected from the rectum of each rodent, and DNA was extracted from them. The rodent species was identified by PCR amplifying the vertebrate <jats:italic>cytochrome b<\/jats:italic> gene. <jats:italic>Cryptosporidium<\/jats:italic> was detected by PCR amplification and amplicon sequencing the small subunit of ribosomal RNA gene. Positive samples of <jats:italic>C. viatorum<\/jats:italic> and <jats:italic>C. parvum<\/jats:italic> were further subtyped by analyzing the 60-kDa glycoprotein gene. A positive <jats:italic>Cryptosporidium<\/jats:italic> result was found in 7% (26\/370) of samples, involving five rodent species: <jats:italic>Apodemus agrarius<\/jats:italic> (36), <jats:italic>Niviventer niviventer<\/jats:italic> (75), <jats:italic>Rattus losea<\/jats:italic> (18), <jats:italic>R. norvegicus<\/jats:italic> (155), and <jats:italic>R. tanezumi<\/jats:italic> (86). Their respective <jats:italic>Cryptosporidium<\/jats:italic> positive rates were 8.3%, 5.3%, 11.1%, 7.1%, and 7.0%. Sequence analysis confirmed the presence of three <jats:italic>Cryptosporidium<\/jats:italic> species: <jats:italic>C. parvum<\/jats:italic> (4), <jats:italic>C. viatorum<\/jats:italic> (1), and <jats:italic>C. muris<\/jats:italic> (1), and two genotypes: <jats:italic>Cryptosporidium<\/jats:italic> rat genotype IV (16) and <jats:italic>C. mortiferum<\/jats:italic>-like (4). Additionally, two subtypes of <jats:italic>C. parvum<\/jats:italic> (IIdA15G1 and IIpA19) and one subtype of <jats:italic>C. viatorum<\/jats:italic> (XVdA3) were detected. These results demonstrate that various wild rodent species in Zhejiang were concurrently infected with rodent-adapted and zoonotic species\/genotypes of <jats:italic>Cryptosporidium<\/jats:italic>, indicating that these rodents can play a role in maintaining and dispersing this parasite into the environment and other hosts, including humans.<\/jats:p>","DOI":"10.1051\/parasite\/2024033","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T08:05:54Z","timestamp":1719821154000},"page":"34","source":"Crossref","is-referenced-by-count":8,"title":["Cross-species transmission of <i>Cryptosporidium<\/i> in wild rodents from the southern region of Zhejiang Province of China and its possible impact on public health"],"prefix":"10.1051","volume":"31","author":[{"given":"Yanyan","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiying","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangxu","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianming","family":"Xin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongying","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiani","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanbin","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huicong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2024,7,1]]},"reference":[{"issue":"6","key":"R1","doi-asserted-by":"crossref","first-page":"2744","DOI":"10.1128\/JCM.41.6.2744-2747.2003","volume":"41","author":"Alves","year":"2003","journal-title":"Journal 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Production"}],"container-title":["Parasite"],"original-title":[],"link":[{"URL":"https:\/\/www.parasite-journal.org\/10.1051\/parasite\/2024033\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T08:06:28Z","timestamp":1719821188000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.parasite-journal.org\/10.1051\/parasite\/2024033"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":40,"alternative-id":["parasite240045"],"URL":"https:\/\/doi.org\/10.1051\/parasite\/2024033","relation":{},"ISSN":["1776-1042"],"issn-type":[{"value":"1776-1042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]}}}