{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T21:05:37Z","timestamp":1765487137326,"version":"3.37.3"},"reference-count":34,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T00:00:00Z","timestamp":1724889600000},"content-version":"vor","delay-in-days":241,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172882"],"award-info":[{"award-number":["32172882"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Key Research and Development Plan Project of China","award":["2022YFD1800200"],"award-info":[{"award-number":["2022YFD1800200"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2024,7,26]]},"published-print":{"date-parts":[[2024]]},"abstract":"<jats:p><jats:italic>Cryptosporidium<\/jats:italic> is a globally distributed zoonotic protozoan parasite that can cause severe diarrhea in humans and animals. L-type lectins are carbohydrate-binding proteins involved in multiple pathways in animals and plants, including protein transportation, secretion, innate immunity, and the unfolded protein response signaling pathway. However, the biological function of the L-type lectins remains unknown in <jats:italic>Cryptosporidium parvum<\/jats:italic>. Here, we preliminarily characterized an L-type lectin in <jats:italic>C. parvum<\/jats:italic> (CpLTL) that contains a lectin-leg-like domain. Immunofluorescence assay confirmed that CpLTL is located on the wall of oocysts, the surface of the mid-anterior region of the sporozoite and the cytoplasm of merozoites. The involvement of CpLTL in parasite invasion is partly supported by experiments showing that an anti-CpLTL antibody could partially block the invasion of <jats:italic>C. parvum<\/jats:italic> sporozoites into host cells. Moreover, the recombinant CpLTL showed binding ability with mannose and the surface of host cells, and competitively inhibited the invasion of <jats:italic>C. parvum<\/jats:italic>. Two host cell proteins were identified by proteomics which should be prioritized for future validation of CpLTL-binding. Our data indicated that CpLTL is potentially involved in the adhesion and invasion of <jats:italic>C. parvum<\/jats:italic>.<\/jats:p>","DOI":"10.1051\/parasite\/2024051","type":"journal-article","created":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T08:02:27Z","timestamp":1725004947000},"page":"51","source":"Crossref","is-referenced-by-count":1,"title":["A single-pass type I membrane protein, mannose-specific L-type lectin, potentially involved in the adhesion and invasion of <i>Cryptosporidium parvum<\/i>"],"prefix":"10.1051","volume":"31","author":[{"given":"Xiaotian","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songying","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenchao","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luyang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guanda","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuexin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baiyi","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5706-131X","authenticated-orcid":false,"given":"Longxian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoying","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"250","published-online":{"date-parts":[[2024,8,29]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1186\/1471-2334-9-195","volume":"9","author":"Amadi","year":"2009","journal-title":"BMC 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