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Recent research indicates associations between altered biliary microbiota and bile duct disorders. However, the impacts of<jats:italic>C. sinensis<\/jats:italic>infection on bile duct epithelium and subsequent effects on biliary microbiota remain unknown.<jats:italic>Methods:<\/jats:italic>Feline bile duct samples were collected from both uninfected and<jats:italic>C. sinensis<\/jats:italic>-infected cats. Histopathological examination was performed to assess epithelial changes, fibrosis, mucin and cell proliferation using hematoxylin-eosin staining and immunohistochemistry. Additionally, biliary microbiota composition was analyzed through 16S rRNA gene sequencing. Statistical analyses were conducted to compare the microbial diversity and relative abundance between infected and uninfected samples.<jats:italic>Results:<\/jats:italic>Histopathological analysis of infected feline bile ducts revealed prominent epithelial hyperplasia characterized by increased cell proliferation. Moreover, periductal fibrosis and collagen fibrosis were observed in infected samples compared to uninfected controls. Biliary microbial richness decreased with disease progression compared to uninfected controls.<jats:italic>Streptococcus<\/jats:italic>abundance positively correlated with disease severity, dominating communities in cancer samples. Predictive functional analysis suggested that<jats:italic>C. sinensis<\/jats:italic>may promote bile duct lesions by increasing microbial genes for carbohydrate metabolism, replication, and repair.<jats:italic>Conclusions:<\/jats:italic>This study provides comprehensive insights into the pathological effects of<jats:italic>C. sinensis<\/jats:italic>infection on feline bile duct epithelium and its influence on biliary microbiota composition. These novel findings provide insight into<jats:italic>C. sinensis<\/jats:italic>pathogenesis and could inform therapeutic development against human clonorchiasis. Further research is warranted to elucidate the underlying mechanisms driving these changes and their implications for host-parasite interactions.<\/jats:p>","DOI":"10.1051\/parasite\/2024053","type":"journal-article","created":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T08:04:21Z","timestamp":1725609861000},"page":"53","source":"Crossref","is-referenced-by-count":2,"title":["<i>Clonorchis sinensis<\/i>infection induces pathological changes in feline bile duct epithelium and alters biliary microbiota composition"],"prefix":"10.1051","volume":"31","author":[{"given":"Feng","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanli","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunfu","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fenqi","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baojiang","family":"Gan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4120-7759","authenticated-orcid":false,"given":"Xinyu","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"250","published-online":{"date-parts":[[2024,9,6]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Abdullah H, Ahmad MF, Maniyam MN, Azman HH, Yaacob NS. 2023. 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