{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:09:33Z","timestamp":1790712573651,"version":"4.1.0"},"reference-count":47,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T00:00:00Z","timestamp":1728432000000},"content-version":"vor","delay-in-days":282,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2024,9,18]]},"published-print":{"date-parts":[[2024]]},"abstract":"<jats:p><jats:italic>Angiostrongylus cantonensis<\/jats:italic>, commonly known as the rat lungworm, causes Eosinophilic meningitis in humans. Our study aimed to investigate the prevalence and distribution of this parasite in rats in Haiti. Rats were trapped at 8 sites, 7 in Artibonite (rural region) and one in an urban area of Port-au-Prince. After euthanasia, hearts and lungs were sampled and preserved in 70% ethanol. Subsequently, the organs were dissected to detect adult worms. Parasite DNA was amplified using PCR targeting either the nematode ITS2 gene for rodent lung tissue or <jats:italic>cox1<\/jats:italic> for isolated worms. Subsequent sequencing allowed parasite identification. A total of 70 rats were captured, <jats:italic>i.e.<\/jats:italic> 23 <jats:italic>Rattus norvegicus<\/jats:italic> and 47 <jats:italic>Rattus rattus.<\/jats:italic> Adult nematodes morphologically compatible with <jats:italic>A. cantonensis<\/jats:italic> were isolated from 5\/70 rats (7%) and identification was confirmed by sequencing. Molecular analysis of lung tissue revealed a parasite prevalence of 31.4% (22\/70), and its presence at 4 of the 8 sites investigated, including Port-au-Prince. The molecular approach on lung tissue targeting the ITS2 gene enabled us to detect a prevalence 4 times higher than the visual search for adult worms alone. Only one COX1 haplotype was identified, belonging to genotype II-G, widely distributed in Brazil, the French Antilles (Guadeloupe), French Polynesia, Hawaii, and Japan. These results confirm that <jats:italic>A. cantonensis<\/jats:italic> is an endemic parasite in Haiti not only in the capital Port-au-Prince, but also in several rural areas. Direct molecular screening for <jats:italic>Angiostrongylus<\/jats:italic> DNA in rat lung tissue showed higher sensitivity than visual detection of worms during dissection and could be useful for further prevalence studies.<\/jats:p>","DOI":"10.1051\/parasite\/2024063","type":"journal-article","created":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T07:55:00Z","timestamp":1728546900000},"page":"64","source":"Crossref","is-referenced-by-count":1,"title":["Molecular study of the status of <i>Angiostrongylus cantonensis<\/i> in rats in Haiti"],"prefix":"10.1051","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9883-9629","authenticated-orcid":false,"given":"Jimmy","family":"Fedna","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romain","family":"Borne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Rieffel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2242-0060","authenticated-orcid":false,"given":"Gudrun","family":"Bornette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Hugues","family":"Henrys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6001-3135","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9ric","family":"Grenouillet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9566-9599","authenticated-orcid":false,"given":"Francis","family":"Raoul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2024,10,9]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"319","DOI":"10.4269\/ajtmh.1986.35.319","volume":"35","author":"Andersen","year":"1986","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"R2","unstructured":"Anderson RC. 1978. Keys to genera of the superfamily Metastrongyloidea. CIH Keys to the Nematode Parasites of Vertebrates No. 5. Farnham Royal, Slough, UK: Commonwealth Agricultural Bureaux."},{"key":"R3","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1097\/QCO.0000000000000481","volume":"31","author":"Ansdell","year":"2018","journal-title":"Current Opinion in Infectious Diseases"},{"key":"R4","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1017\/S0031182016000652","volume":"143","author":"Barratt","year":"2016","journal-title":"Parasitology"},{"key":"R5","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.vetpar.2009.02.020","volume":"162","author":"Chikweto","year":"2009","journal-title":"Veterinary Parasitology"},{"key":"R6","first-page":"298","volume":"18","author":"Companioni","year":"2019","journal-title":"Cuba. Revista Habanera de Ciencias M\u00e9dicas"},{"key":"R7","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/bs.apar.2023.05.003","volume":"121","author":"Cowie","year":"2023","journal-title":"Advances in Parasitology"},{"key":"R8","first-page":"210","volume":"10","author":"Criscuolo","year":"2010","journal-title":"BMC Ecology and Evolution"},{"key":"R9","doi-asserted-by":"crossref","first-page":"692","DOI":"10.4269\/ajtmh.16-0792","volume":"96","author":"Dard","year":"2017","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"R10","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1051\/parasite\/2020032","volume":"27","author":"Dard","year":"2020","journal-title":"Parasite"},{"key":"R11","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.ijid.2020.01.012","volume":"93","author":"Federspiel","year":"2020","journal-title":"International Journal of Infectious Diseases"},{"key":"R12","doi-asserted-by":"crossref","first-page":"e48374","DOI":"10.1371\/journal.pone.0048374","volume":"7","author":"Galan","year":"2012","journal-title":"PLoS One"},{"key":"R13","doi-asserted-by":"crossref","first-page":"2564","DOI":"10.3201\/eid2812.220418","volume":"28","author":"Galan-Puchades","year":"2022","journal-title":"Emerging Infectious Diseases"},{"key":"R14","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1186\/s13071-023-05872-4","volume":"16","author":"Gamiette","year":"2023","journal-title":"Parasites & Vectors"},{"key":"R15","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.1093\/nar\/21.10.2525","volume":"21","author":"Gasser","year":"1993","journal-title":"Nucleic Acids Research"},{"key":"R16","doi-asserted-by":"crossref","first-page":"e05150","DOI":"10.1016\/j.heliyon.2020.e05150","volume":"6","author":"Goncalves da Mota","year":"2020","journal-title":"Brazil. Heliyon"},{"key":"R17","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.3201\/eid2910.230706","volume":"29","author":"Gottdenker","year":"2023","journal-title":"Emerging Infectious Diseases"},{"key":"R18","first-page":"185","volume":"20","author":"Guo","year":"2022","journal-title":"Journal of Tropical Diseases and Parasitology"},{"key":"R19","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1111\/zph.13163","volume":"71","author":"Hancke","year":"2024","journal-title":"Zoonoses and Public Health"},{"key":"R20","doi-asserted-by":"crossref","unstructured":"Hardy AR, Quy RJ, Huson LW. 1983. Estimation of age in the Norway rat (Rattus norvegicus Berkenhout) from the weight of the eyelens. Journal of Applied Ecology, 97\u2013102.","DOI":"10.2307\/2403378"},{"key":"R21","unstructured":"Herbreteau V, Jittapalapong S, Rerkamnuaychoke W, Chaval Y, Cosson JF, Morand S. 2011. Protocols for field and laboratory rodent studies, 2011. CERoPath Project. Available at http:\/\/www.ceropath.org\/about\/project_overview."},{"key":"R22","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1093\/bioinformatics\/17.8.754","volume":"17","author":"Huelsenbeck","year":"2001","journal-title":"Bioinformatics"},{"key":"R23","unstructured":"IHSI M. 2015. Population totale de 18 ans et plus, m\u00e9nages, et densit\u00e9s estim\u00e9es en 2015. Institut Haitien de Statistique et d\u2019Informatique (IHSI), Haiti."},{"key":"R24","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","volume":"30","author":"Katoh","year":"2013","journal-title":"Molecular Biology and Evolution"},{"key":"R25","doi-asserted-by":"crossref","first-page":"W260","DOI":"10.1093\/nar\/gkz303","volume":"47","author":"Lemoine","year":"2019","journal-title":"Nucleic Acids Research"},{"key":"R26","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1590\/S0074-02762010000700019","volume":"105","author":"Maldonado Junior","year":"2010","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"},{"key":"R27","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.actatropica.2012.10.001","volume":"125","author":"Moreira","year":"2013","journal-title":"Achatina fulica. Acta Tropica"},{"key":"R28","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/j.1365-2907.1972.tb00160.x","volume":"2","author":"Morris","year":"1972","journal-title":"Mammal Review"},{"key":"R29","first-page":"1247","volume":"38","author":"Nagai","year":"1990","journal-title":"Japanese Journal of Clinical Pathology"},{"key":"R30","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.3201\/eid2306.161999","volume":"23","author":"Nguyen","year":"2017","journal-title":"Emerging Infectious Diseases"},{"key":"R31","doi-asserted-by":"crossref","first-page":"322","DOI":"10.7589\/2013-03-050","volume":"50","author":"Okano","year":"2014","journal-title":"Journal of Wildlife Diseases"},{"key":"R32","doi-asserted-by":"crossref","first-page":"857","DOI":"10.4269\/ajtmh.22-0782","volume":"108","author":"Paredes-Esquivel","year":"2023","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"R33","doi-asserted-by":"crossref","first-page":"187","DOI":"10.2174\/187221508786241738","volume":"2","author":"Pereira","year":"2008","journal-title":"Recent Patents on DNA & Gene Sequences (Discontinued)"},{"key":"R34","first-page":"63","volume":"72","author":"Qvarnstrom","year":"2013","journal-title":"Hawai\u2019i Journal of Medicine & Public Health"},{"key":"R35","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1046\/j.1365-3156.2003.01035.x","volume":"8","author":"Raccurt","year":"2003","journal-title":"Tropical Medicine & International Health"},{"key":"R36","doi-asserted-by":"crossref","first-page":"e0161128","DOI":"10.1371\/journal.pone.0161128","volume":"11","author":"Rodpai","year":"2016","journal-title":"Plos One"},{"key":"R37","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/S0020-7519(97)00213-0","volume":"28","author":"Romstad","year":"1998","journal-title":"International Journal for Parasitology"},{"key":"R38","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1186\/1756-0500-6-513","volume":"6","author":"San Mill\u00e1n","year":"2013","journal-title":"BMC Research Notes"},{"key":"R39","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1644\/10-MAMM-F-355.1","volume":"92","author":"Sikes","year":"2011","journal-title":"Journal of Mammalogy"},{"key":"R40","first-page":"49","volume":"18","author":"Tanikawa","year":"1993","journal-title":"Journal of the Mammalogical Society of Japan"},{"key":"R41","doi-asserted-by":"crossref","first-page":"788","DOI":"10.3390\/pathogens12060788","volume":"12","author":"Tian","year":"2023","journal-title":"Pathogens"},{"key":"R42","doi-asserted-by":"crossref","first-page":"100426","DOI":"10.1016\/j.onehlt.2022.100426","volume":"15","author":"Turck","year":"2022","journal-title":"One Health"},{"key":"R43","doi-asserted-by":"crossref","first-page":"237","DOI":"10.2307\/3281599","volume":"72","author":"Ubelaker","year":"1986","journal-title":"Journal of Parasitology"},{"key":"R44","first-page":"253","volume":"43","author":"Vargas","year":"1992","journal-title":"Tropical Medicine and Parasitology"},{"key":"R45","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/s10096-011-1328-5","volume":"31","author":"Wang","year":"2012","journal-title":"European Journal of Clinical Microbiology & Infectious Diseases"},{"key":"R46","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1017\/S0031182016000640","volume":"143","author":"Waugh","year":"2016","journal-title":"Parasitology"},{"key":"R47","doi-asserted-by":"crossref","first-page":"1228206","DOI":"10.3389\/fcimb.2023.1228206","volume":"13","author":"Ye","year":"2023","journal-title":"Frontiers in Cellular and Infection Microbiology"}],"container-title":["Parasite"],"original-title":[],"link":[{"URL":"https:\/\/www.parasite-journal.org\/10.1051\/parasite\/2024063\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T19:48:59Z","timestamp":1737748139000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.parasite-journal.org\/10.1051\/parasite\/2024063"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":47,"alternative-id":["parasite240038"],"URL":"https:\/\/doi.org\/10.1051\/parasite\/2024063","relation":{},"ISSN":["1776-1042"],"issn-type":[{"value":"1776-1042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]}}}