{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:31:34Z","timestamp":1790253094477,"version":"4.1.0"},"reference-count":40,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"vor","delay-in-days":47,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan","doi-asserted-by":"crossref","award":["242300421590"],"award-info":[{"award-number":["242300421590"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Key Research Projects of Higher Education Institutions in Henan Province","award":["24A230017"],"award-info":[{"award-number":["24A230017"]}]},{"name":"Key Research Projects of Xuchang University","award":["2024ZD013"],"award-info":[{"award-number":["2024ZD013"]}]},{"name":"Open Project of Tarim Animal Disease Diagnosis and Control Engineering Laboratory of Xinjiang Production and Construction Corps","award":["ELDC202301"],"award-info":[{"award-number":["ELDC202301"]}]},{"name":"Research Projects of Xuchang University","award":["2024YB017"],"award-info":[{"award-number":["2024YB017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2025,1,29]]},"published-print":{"date-parts":[[2025]]},"abstract":"<jats:p><jats:italic>Blastocystis<\/jats:italic> sp. is a zoonotic intestinal protozoan that is ubiquitous globally, residing in the gastrointestinal tracts of both humans and various animals. In the present study, a PCR-sequencing tool based on the SSU rRNA gene was employed to investigate the prevalence and subtypes of <jats:italic>Blastocystis<\/jats:italic> spp. in 204 fresh fecal samples collected from 20 captive wildlife species from a bird park in Henan Province, Central China. Overall, <jats:italic>Blastocystis<\/jats:italic> was present in 13.73% (28 out of 204) of the samples and 25% (5 out of 20) of the species. A total of four zoonotic subtypes of <jats:italic>Blastocystis<\/jats:italic> sp. were found: ST1, ST3, ST5, and ST27, with the latter being the most prevalent, accounting for 35.71% (10 out of 28) of the 5 species positive for <jats:italic>Blastocystis<\/jats:italic> sp. To the best of our knowledge, this is the first report of <jats:italic>Blastocystis<\/jats:italic> ST27 in birds in China, namely bar-headed goose (<jats:italic>Anser indicus)<\/jats:italic> and peafowl (<jats:italic>Pavo muticus<\/jats:italic>). The data suggest that captive wildlife, particularly those in bird parks, may frequently be infected with this zoonotic pathogen. Consequently, these animals may serve as potential reservoirs for zoonotic infections in humans.<\/jats:p>","DOI":"10.1051\/parasite\/2025006","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T08:45:19Z","timestamp":1739781919000},"page":"11","source":"Crossref","is-referenced-by-count":6,"title":["Molecular characterization and subtype analysis of <i>Blastocystis<\/i> sp. in captive wildlife in Henan, China"],"prefix":"10.1051","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4628-0533","authenticated-orcid":false,"given":"Zhaohui","family":"Cui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyao","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaifang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aili","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qichen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2025,2,17]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1186\/s13071-015-1071-3","volume":"8","author":"Abu-Madi","year":"2015","journal-title":"Parasites & Vectors"},{"key":"R2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.meegid.2017.02.016","volume":"50","author":"Alinaghizade","year":"2017","journal-title":"Infection, Genetics and Evolution"},{"issue":"6","key":"R3","doi-asserted-by":"crossref","first-page":"853","DOI":"10.3390\/ani14060853","volume":"14","author":"An","year":"2024","journal-title":"Animals"},{"issue":"3","key":"R4","doi-asserted-by":"crossref","first-page":"461","DOI":"10.3390\/microorganisms12030461","volume":"12","author":"Aykur","year":"2024","journal-title":"Microorganisms"},{"key":"R5","doi-asserted-by":"crossref","first-page":"105770","DOI":"10.1016\/j.prevetmed.2022.105770","volume":"208","author":"Barati","year":"2022","journal-title":"Preventive Veterinary Medicine"},{"key":"R6","doi-asserted-by":"crossref","first-page":"641483","DOI":"10.3389\/fmicb.2021.641483","volume":"12","author":"Billy","year":"2021","journal-title":"Frontiers in Microbiology"},{"issue":"8","key":"R7","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1038\/nrgastro.2014.40","volume":"11","author":"Collins","year":"2014","journal-title":"Nature Reviews Gastroenterology & Hpatology"},{"key":"R8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijppaw.2023.07.005","volume":"22","author":"Cruz-Saavedra","year":"2023","journal-title":"International Journal for Parasitology \u2013 Parasites and Wildlife"},{"issue":"2","key":"R9","doi-asserted-by":"crossref","first-page":"e1009253","DOI":"10.1371\/journal.ppat.1009253","volume":"17","author":"Deng","year":"2021","journal-title":"PLoS Pthogens"},{"issue":"6","key":"R10","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.4269\/ajtmh.12-0777","volume":"88","author":"El Safadi","year":"2013","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"issue":"2","key":"R11","doi-asserted-by":"crossref","first-page":"e13012","DOI":"10.1111\/jeu.13012","volume":"71","author":"Feng","year":"2024","journal-title":"Journal of Eukaryotic Microbiology"},{"key":"R12","doi-asserted-by":"crossref","unstructured":"Fu X, Lyu J, Shi Y, Cao B, Liu D, Yang X, Huang L, Liang Q, Liao D, He S. 2024. 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