{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T04:06:44Z","timestamp":1750824404067,"version":"3.41.0"},"reference-count":32,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:00:00Z","timestamp":1750723200000},"content-version":"vor","delay-in-days":174,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"not applicable","award":["not applicable"],"award-info":[{"award-number":["not applicable"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2025,6,2]]},"published-print":{"date-parts":[[2025]]},"abstract":"<jats:p><jats:italic>Amblyomma maculatum<\/jats:italic>, the Gulf Coast tick, is a species of significant veterinary and public health importance, especially because it is a vector of important diseases, such as American canine hepatozoonosis and tidewater spotted fever. <jats:italic>Amblyomma maculatum<\/jats:italic> infests a wide range of vertebrates including livestock, dogs, cats, and humans. Two experimental studies were conducted to evaluate the efficacy of afoxolaner formulated in an oral tablet (NexGard<jats:sup>\u00ae<\/jats:sup>) against induced infestations of <jats:italic>A. maculatum<\/jats:italic> in dogs. These Good Clinical Practice (GCP) studies used a randomized, negative controlled and masked design. In each study, 10 dogs were allocated to an untreated group and 10 dogs to a treated group, dosed once on Day 0 with a combination of tablets targeting the minimum therapeutic dose (2.5\u00a0mg\/kg afoxolaner). Dogs were infested with 50 unfed adult <jats:italic>A. maculatum<\/jats:italic> on Days \u22122, 7, 14, 21, 28, and 35 (Study #1), or on Days \u22121, 14, and 28 (Study #2). Seventy-two (72) hours after treatment and subsequent infestations, ticks were removed and the numbers of live ticks in each group were used for efficacy calculations. At each time-point, all untreated dogs were adequately infested (<jats:italic>i.e.<\/jats:italic>, with more than 12 live ticks), demonstrating a vigorous tick population and an adequate study model. The curative efficacy against established infestations, 72 hours after treatment, was 100% in Study #1 and 99.5% in Study #2. The preventive efficacy, 72 hours after the post-treatment infestations, ranged from 94.6% to 98.9% for five weeks in Study #1, and was \u226598.8% for four weeks in Study #2.<\/jats:p>","DOI":"10.1051\/parasite\/2025032","type":"journal-article","created":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T07:52:37Z","timestamp":1750751557000},"page":"39","source":"Crossref","is-referenced-by-count":0,"title":["Efficacy of oral afoxolaner against <i>Amblyomma maculatum<\/i> infestations in 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