{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T09:01:12Z","timestamp":1764147672090,"version":"3.46.0"},"reference-count":27,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":329,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2025,10,30]]},"published-print":{"date-parts":[[2025]]},"abstract":"<jats:p>\n                    Congenital toxoplasmosis is a fetal infection resulting from the transplacental transmission of\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    in mothers who seroconvert during pregnancy. Neonatal diagnosis has recently been improved through the identification by L\u2019Ollivier\n                    <jats:italic>et al.<\/jats:italic>\n                    (2012) and Peyclit\n                    <jats:italic>et al.<\/jats:italic>\n                    (2023) of a pathognomonic marker for congenital toxoplasmosis: the IgM triplet, corresponding to three high molecular weight bands of 75, 90, and 100\u00a0kDa, respectively found on the mother-child immunoblot pair profile. This is a new concept, as these three IgM bands reflect an immune response targeting proteins involved in vertical transmission of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    . These proteins may be\n                    <jats:italic>T. gondii<\/jats:italic>\n                    secreted or non-secreted effectors implicated in host cell invasion, immune modulation, and parasite virulence. In this study, immunoproteomic techniques allowed us to identify thirty-two relevant protein spots on immunoblot, including four specifically associated with the IgM triplet. Protein identification by LC-MS\/MS revealed several\n                    <jats:italic>T. gondii<\/jats:italic>\n                    proteins as strong candidates for the IgM triplet. Each of these proteins is, directly or indirectly, involved in cellular invasion and may also play a role in transplacental transmission of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    . Identifying these proteins opens several avenues of therapeutic research that could improve the management of congenital toxoplasmosis.\n                  <\/jats:p>","DOI":"10.1051\/parasite\/2025065","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T08:56:38Z","timestamp":1764147398000},"page":"72","source":"Crossref","is-referenced-by-count":0,"title":["Protein characterization of the IgM triplet involved in the diagnosis of congenital toxoplasmosis"],"prefix":"10.1051","volume":"32","author":[{"given":"Maria","family":"Carreno","sequence":"first","affiliation":[{"name":"IHU M\u00e9diterran\u00e9e Infection","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odile","family":"Villard","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie et Mycologie M\u00e9dicale, Laboratoire Associ\u00e9 CNR de la Toxoplasmose, Les H\u00f4pitaux Universitaires de Strasbourg","place":["France"]},{"name":"Institut de Parasitologie et Pathologie Tropicale, UR7292 Dynamique des interactions h\u00f4te pathog\u00e8ne, F\u00e9d\u00e9ration de M\u00e9decine Translationnelle, Universit\u00e9 de Strasbourg","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7415-4198","authenticated-orcid":false,"given":"Isabelle","family":"Villena","sequence":"additional","affiliation":[{"name":"UR ESCAPE, Universit\u00e9 Reims-Champagne-Ardenne, Service de Parasitologie-Mycologie, Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Centre Hospitalier Universitaire (CHU) Reims","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucie","family":"Peyclit","sequence":"additional","affiliation":[{"name":"IHU M\u00e9diterran\u00e9e Infection","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helene","family":"Diemer","sequence":"additional","affiliation":[{"name":"Laboratoire de Spectrom\u00e9trie de Masse BioOrganique, Universit\u00e9 de Strasbourg, CNRS, IPHC UMR7178","place":["France"]},{"name":"Infrastructure Nationale de Prot\u00e9omique ProFI, UAR2048","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Schaeffer-Reiss","sequence":"additional","affiliation":[{"name":"Laboratoire de Spectrom\u00e9trie de Masse BioOrganique, Universit\u00e9 de Strasbourg, CNRS, IPHC UMR7178","place":["France"]},{"name":"Infrastructure Nationale de Prot\u00e9omique ProFI, UAR2048","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Coralie","family":"L\u2019Ollivier","sequence":"additional","affiliation":[{"name":"IHU M\u00e9diterran\u00e9e Infection","place":["France"]},{"name":"Aix Marseille Univ, IRD, AP-HM, SSA, VITROME","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"250","published-online":{"date-parts":[[2025,11,26]]},"reference":[{"issue":"5","key":"R1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.chom.2017.04.007","volume":"21","author":"Arora","year":"2017","journal-title":"Cell Host Microbe"},{"key":"R2","unstructured":"Carreno M. 2023. Caract\u00e9risation prot\u00e9ique de la triplette IgM impliqu\u00e9e dans le diagnostic de la toxoplasmose cong\u00e9nitale. Th\u00e8se de doctorat, Universit\u00e9 d\u2019Aix-Marseille. Dumas-04191507. 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