{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T16:04:51Z","timestamp":1783008291672,"version":"3.54.5"},"reference-count":76,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"vor","delay-in-days":331,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100007917","name":"Agricultural Research Service","doi-asserted-by":"publisher","award":["58-3022-1-018-F (Risk of Arthropod-borne diseases in the Caribbean)"],"award-info":[{"award-number":["58-3022-1-018-F (Risk of Arthropod-borne diseases in the Caribbean)"]}],"id":[{"id":"10.13039\/100007917","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2025,11,3]]},"published-print":{"date-parts":[[2025]]},"abstract":"<jats:p>\n                    The tropical bont tick,\n                    <jats:italic>Amblyomma variegatum<\/jats:italic>\n                    , is endemic in sub-Saharan and southern Africa, as well as several Caribbean islands. This tick is deleterious for the general health of ruminants and is the primary vector of\n                    <jats:italic>Ehrlichia ruminantium<\/jats:italic>\n                    , the causative agent of heartwater. Understanding the ecology and biology of\n                    <jats:italic>A. variegatum<\/jats:italic>\n                    is thus crucial to describe tick-host-bacteria interactions and thus develop effective control methods. To better understand vector competence, detailed functional characterization and precise isolation of tick organs is needed. All procedures described in this study were performed using a simple, high-quality binocular magnifying glass. Following this approach, we present descriptions and illustrations of the internal anatomy\u00a0of\n                    <jats:italic>A. variegatum<\/jats:italic>\n                    at male, female (at various stages of engorgement), and nymph stages. This study focused on critical tissues associated with vector competence, including midguts, salivary glands, or ovaries, which were targeted and isolated. We identified morphological differences in the reproductive systems of both\n                    <jats:italic>A. variegatum<\/jats:italic>\n                    male and female ticks when compared with other tick species. We also provide numerous practical and technical aspects for obtaining organs suitable for detailed studies. The method presented here ensures organs of high quality, without degradation or contamination, as required for cellular or molecular studies on host-vector-pathogen interactions.\n                  <\/jats:p>","DOI":"10.1051\/parasite\/2025068","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T08:53:20Z","timestamp":1764320000000},"page":"74","source":"Crossref","is-referenced-by-count":2,"title":["Dissection and internal anatomy of the giant tropical bont tick\n                    <i>Amblyomma variegatum<\/i>"],"prefix":"10.1051","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1094-983X","authenticated-orcid":false,"given":"Naomie","family":"Pature","sequence":"first","affiliation":[{"name":"CIRAD, UMR ASTRE","place":["France"]},{"name":"ASTRE, CIRAD, INRAE, Universit\u00e9 de Montpellier","place":["France"]},{"name":"Universit\u00e9 des Antilles, \u00c9cole doctorale n\u00b0 636 DEECA, Campus de Fouillole","place":["France"]},{"name":"CaribVET, site de Duclos","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6020-7175","authenticated-orcid":false,"given":"Nonito","family":"Pag\u00e8s","sequence":"additional","affiliation":[{"name":"CIRAD, UMR ASTRE","place":["France"]},{"name":"ASTRE, CIRAD, INRAE, Universit\u00e9 de Montpellier","place":["France"]},{"name":"CaribVET, site de Duclos","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8390-4931","authenticated-orcid":false,"given":"Val\u00e9rie","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"CIRAD, UMR ASTRE","place":["France"]},{"name":"ASTRE, CIRAD, INRAE, Universit\u00e9 de Montpellier","place":["France"]},{"name":"CaribVET, site de Duclos","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2735-176X","authenticated-orcid":false,"given":"Damien F.","family":"Meyer","sequence":"additional","affiliation":[{"name":"CIRAD, UMR ASTRE","place":["France"]},{"name":"ASTRE, CIRAD, INRAE, Universit\u00e9 de Montpellier","place":["France"]},{"name":"CaribVET, site de Duclos","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2025,11,28]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1007\/s00436-011-2422-4","volume":"109","author":"Abdel-Shafy","year":"2011","journal-title":"Parasitology Research"},{"key":"R2","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.14202\/vetworld.2016.1087-1101","volume":"9","author":"Abdullah","year":"2016","journal-title":"Veterinary World"},{"key":"R3","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1007\/s00436-024-08284-0","volume":"123","author":"Abouelhassan","year":"2024","journal-title":"Parasitology Research"},{"issue":"1","key":"R4","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1159\/000535986","volume":"16","author":"Adegoke","year":"2023","journal-title":"Journal of Innate Immunity"},{"key":"R5","doi-asserted-by":"crossref","first-page":"100066","DOI":"10.1016\/j.crpvbd.2021.100066","volume":"2","author":"Aguilar-D\u00edaz","year":"2022","journal-title":"Current Research in Parasitology & Vector-Borne Diseases"},{"key":"R6","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1111\/azo.12083","volume":"96","author":"Anholeto","year":"2015","journal-title":"Acta Zoologica"},{"key":"R7","first-page":"63","volume":"36","author":"Arthur","year":"1973","journal-title":"Journal of the Entomological Society of Southern Africa"},{"key":"R8","doi-asserted-by":"crossref","first-page":"341","DOI":"10.2307\/3226866","volume":"116","author":"Baker","year":"1997","journal-title":"Invertebrate Biology"},{"key":"R9","unstructured":"Barr\u00e9 N. 1989. 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