{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T20:33:31Z","timestamp":1793824411423,"version":"4.6.1"},"reference-count":76,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"vor","delay-in-days":338,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2025,11,11]]},"published-print":{"date-parts":[[2025]]},"abstract":"<jats:p>\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    is one of the most prevalent intestinal protozoans, transmitted through the fecal-oral route. Domestic pigs and cattle serve as important reservoirs for\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    , playing a crucial role in its transmission dynamics. In the present study, a PCR-sequencing tool based on the small subunit ribosomal RNA (\n                    <jats:italic>SSU<\/jats:italic>\n                    rRNA) gene was employed to investigate the prevalence and subtypes of\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    in 456 pig and 302 cattle fecal samples collected in Hainan, the only tropical island province in China. The overall prevalence of\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    in pigs and cattle was 30.3% (138\/456) and 13.2% (40\/302), respectively. Six known subtypes-ST5 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a0139), ST21 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a018), ST26 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a010), ST10 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a07), ST23 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a02), and ST25 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a02)-were identified, including 138 ST5 from pigs, and 18 ST21, 10 ST26, 7 ST10, 2 ST23, 2 ST25, and 1 ST5 from cattle. A novel ST5 sequence (OQ048307) from a pig and a novel ST10 sequence (OQ048308) from a cow were detected. Our results suggest that livestock may be\u00a0an important potential reservoir for zoonotic\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    infection in humans and provided reliable data for future research on subtype distribution and infection control of this protozoan in tropical regions.\n                  <\/jats:p>","DOI":"10.1051\/parasite\/2025070","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T08:57:27Z","timestamp":1764925047000},"page":"77","source":"Crossref","is-referenced-by-count":7,"title":["Molecular characterization and zoonotic potential of\n                    <i>Blastocystis<\/i>\n                    subtypes in domestic pigs and cattle from Hainan, a tropical island province in China"],"prefix":"10.1051","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5025-0275","authenticated-orcid":false,"given":"Yun","family":"Zhang","sequence":"first","affiliation":[{"name":"Key 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