{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T07:36:36Z","timestamp":1781768196320,"version":"3.54.5"},"reference-count":38,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":335,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"the Key Research and Development Project of Jiangxi Province","award":["Grant No. 20203BBF63019"],"award-info":[{"award-number":["Grant No. 20203BBF63019"]}]},{"name":"the Foundation of Key Laboratory of Livestock Infectious Diseases in Northeast China (Shenyang Agricultural University), the Ministry of Education","award":["Grant No. FKLID-2021-01"],"award-info":[{"award-number":["Grant No. FKLID-2021-01"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["Grant No. 31560691"],"award-info":[{"award-number":["Grant No. 31560691"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2025,11,12]]},"published-print":{"date-parts":[[2025]]},"abstract":"<jats:p>\n                    Avian coccidiosis significantly impairs intestinal health in chickens and remains a major threat to the poultry industry worldwide. Frequent co-infections with three\n                    <jats:italic>Eimeria<\/jats:italic>\n                    species,\n                    <jats:italic>i.e.<\/jats:italic>\n                    ,\n                    <jats:italic>Eimeria tenella<\/jats:italic>\n                    ,\n                    <jats:italic>E. necatrix<\/jats:italic>\n                    , and\n                    <jats:italic>E. maxima<\/jats:italic>\n                    , present challenges for effective vaccine development. Here, we first used two-dimensional gel electrophoresis and silver staining to identify 650\n                    <jats:italic>E. tenella<\/jats:italic>\n                    sporozoite proteins and then detected 18 cross-reactive immunogens based on Western blotting and proteomic analysis. These immunogens were consistently recognized by hyperimmune sera raised against three\n                    <jats:italic>Eimeria<\/jats:italic>\n                    species of interest. Bioinformatics analysis revealed that these proteins encompass enzymes, motility-related proteins, nuclear factors, and translation machinery, with amino acid sequence identities ranging from 71.1%\u201398.8% with\n                    <jats:italic>E. necatrix<\/jats:italic>\n                    and 37.9%\u201387.5% with\n                    <jats:italic>E. maxima<\/jats:italic>\n                    homologues. Seven of these proteins show potential non-classical secretion, and four have transmembrane domains. Overall, these findings point to multiple cross-reactive immunogens as potential candidates for multivalent coccidiosis vaccines.\n                  <\/jats:p>","DOI":"10.1051\/parasite\/2025071","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T08:56:00Z","timestamp":1764665760000},"page":"76","source":"Crossref","is-referenced-by-count":1,"title":["Identification of highly cross-reactive immunogens in\n                    <i>Eimeria tenella<\/i>\n                    sporozoites"],"prefix":"10.1051","volume":"32","author":[{"given":"Weiyi","family":"Chen","sequence":"first","affiliation":[{"name":"Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Han","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine\/Research Center of Animal Clinical Medicine, Xinjiang Academy of Animal Science","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Song","sequence":"additional","affiliation":[{"name":"Jiangxi Institute of Science and Technology Information","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haipeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiujiang Lilai Biological Science and Technology Co. LTD, Shacheng Industrial Zone of Jiujiang County","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangqin","family":"Wang","sequence":"additional","affiliation":[{"name":"Agriculture and Rural Bureau of Chaisang District","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiwei","family":"Gong","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2025,12,2]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Andreopoulou M, Chaligiannis I, Sotiraki S, Daugschies A, Bangoura B. Prevalence and molecular detection of Eimeria species in different types of poultry in Greece and associated risk factors. Parasitology Research, 121, 2051\u20132063.","DOI":"10.1007\/s00436-022-07525-4"},{"key":"R2","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/s11686-023-00773-z","volume":"69","author":"Ayadi","year":"2024","journal-title":"Acta Parasitologica"},{"issue":"4","key":"R3","doi-asserted-by":"crossref","first-page":"577","DOI":"10.3390\/ani15040577","volume":"15","author":"Bai","year":"2025","journal-title":"Animals (Basel)"},{"key":"R4","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1007\/s00239-022-10056-2","volume":"90","author":"Barve","year":"2022","journal-title":"Journal of Molecular Evolution"},{"key":"R5","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.meegid.2017.10.009","volume":"55","author":"Blake","year":"2017","journal-title":"Infection, Genetics and Evolution"},{"key":"R6","doi-asserted-by":"crossref","first-page":"140969","DOI":"10.1016\/j.bbapap.2023.140969","volume":"1872","author":"Brangulis","year":"2024","journal-title":"Biochimica et Biophysica Acta. Proteins and Proteomics"},{"key":"R7","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1017\/S0031182019001562","volume":"147","author":"Burrell","year":"2020","journal-title":"Parasitology"},{"key":"R8","doi-asserted-by":"crossref","first-page":"813","DOI":"10.3382\/ps.2008-00316","volume":"88","author":"Chapman","year":"2009","journal-title":"Poultry Science"},{"key":"R9","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/s13567-023-01253-y","volume":"54","author":"Chen","year":"2023","journal-title":"Veterinary Research"},{"key":"R10","doi-asserted-by":"crossref","first-page":"6225","DOI":"10.1016\/j.vaccine.2013.10.034","volume":"31","author":"Chen","year":"2013","journal-title":"Vaccine"},{"key":"R11","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1186\/s13071-023-05828-8","volume":"16","author":"Chen","year":"2023","journal-title":"Parasites & 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