{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T08:07:00Z","timestamp":1779696420410,"version":"3.53.1"},"reference-count":58,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":144,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004397","name":"Ministry of Public Health","doi-asserted-by":"publisher","award":["project code 29647, year 2020"],"award-info":[{"award-number":["project code 29647, year 2020"]}],"id":[{"id":"10.13039\/501100004397","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2026,4,21]]},"published-print":{"date-parts":[[2026]]},"abstract":"<jats:p>\n                    <jats:italic>Trichostrongylus<\/jats:italic>\n                    spp., zoonotic soil-transmitted nematodes, affect both livestock and humans globally. In Thailand, human trichostrongylosis has been reported, but no systematic study examining livestock and humans in the same locality has been conducted. This study aimed to address this gap in Satun Province, southern Thailand by characterizing the disease\u2019s epidemiologic status among livestock, farmers, and the environment using both microscopic and molecular techniques. Human risk factors were assessed via questionnaires. Stool samples were collected from livestock farmers, their families, and herbivores in Nongkhai, Ratchaburi, and Satun Provinces. Vegetable samples were also obtained from households and local markets. Human and livestock feces were examined using the modified Kato\u2013Katz method and a Mini Parasep\n                    <jats:sup>\u00ae<\/jats:sup>\n                    solvent-free fecal parasite concentrator. Total DNA was then extracted from fecal and vegetable sediments, and a 211\u00a0bp fragment of the ribosomal internal transcribed spacer 2 (\n                    <jats:italic>ITS2<\/jats:italic>\n                    ) gene was amplified using a polymerase chain reaction (PCR). PCR products were digested with\n                    <jats:italic>Hinf<\/jats:italic>\n                    I and analyzed via electrophoresis to identify\n                    <jats:italic>Trichostrongylus<\/jats:italic>\n                    species. In Satun,\n                    <jats:italic>Trichostrongylus colubriformis<\/jats:italic>\n                    monoinfection was found in 12 of 221 (5.4%) residents, while co-infection with\n                    <jats:italic>T. colubriformis<\/jats:italic>\n                    and\n                    <jats:italic>T. axei<\/jats:italic>\n                    occurred in 3 (1.4%). Herbivore feces and vegetable sediments from Satun also tested positive. No human cases were found in Nongkhai or Ratchaburi. Risk factors included education level, occupation, and outdoor toilet use. Most infected individuals in Satun were asymptomatic. Despite this, public health interventions should be implemented, targeting the human\u2013livestock\u2013environment interface to control the disease effectively.\n                  <\/jats:p>","DOI":"10.1051\/parasite\/2026027","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T07:49:10Z","timestamp":1779695350000},"page":"29","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence and risk factors of human trichostrongylosis in Satun, southern Thailand"],"prefix":"10.1051","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1933-8493","authenticated-orcid":false,"given":"Teera","family":"Kusolsuk","sequence":"first","affiliation":[{"name":"Department of Helminthology, Faculty of Tropical Medicine, Mahidol University","place":["Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thongroo","family":"Kophachon","sequence":"additional","affiliation":[{"name":"Division of Communicable Diseases, Department 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