{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T08:47:13Z","timestamp":1782204433261,"version":"3.54.5"},"reference-count":35,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":172,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"WACCBIP\/University of Ghana","award":["ACE02-WACCBIP"],"award-info":[{"award-number":["ACE02-WACCBIP"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2026,6,5]]},"published-print":{"date-parts":[[2026]]},"abstract":"<jats:p>\n                    This study aimed to assess the prevalence and risk factors of molecular markers of\n                    <jats:italic>Plasmodium (P.) falciparum<\/jats:italic>\n                    resistance to sulfadoxine-pyrimethamine (SP) among pregnant women in Bobo-Dioulasso, and to examine their association with maternal anaemia. This cross-sectional study was conducted between October and December 2022 among 288 SP-na\u00efve pregnant women attending antenatal care at the Centre M\u00e9dical Urbain of Lafiabougou. Dried blood spots were collected, and\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    infection was detected using quantitative polymerase chain reaction (qPCR) targeting the\n                    <jats:italic>varATS<\/jats:italic>\n                    gene. Positive samples were genotyped for mutations in the\n                    <jats:italic>Pfdhfr<\/jats:italic>\n                    and\n                    <jats:italic>Pfdhps<\/jats:italic>\n                    genes using a nested PCR approach followed by restriction fragment length polymorphism analysis. Multivariable logistic regression models were used to identify predictors of resistance markers and maternal anaemia. Among the 172 qPCR-confirmed\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    \u2013positive samples, the\n                    <jats:italic>Pfdhfr<\/jats:italic>\n                    triple mutant haplotype (N51I, C59R, S108N) was detected in 33.7% of cases, while the\n                    <jats:italic>Pfdhps<\/jats:italic>\n                    A437G mutation was present in 82.7%. No\n                    <jats:italic>Pfdhfr<\/jats:italic>\n                    I164L or\n                    <jats:italic>Pfdhps<\/jats:italic>\n                    K540E mutations were observed. Gestational age was independently associated with carriage of the triple\n                    <jats:italic>Pfdhfr<\/jats:italic>\n                    mutation (adjusted OR = 2.2, 95% CI: 1.1\u20134.5). Both gestational age (adjusted OR = 2.5, 95% CI: 1.2\u20134.9) and infection with parasites carrying the triple\n                    <jats:italic>Pfdhfr<\/jats:italic>\n                    mutation (adjusted OR = 3.9, 95% CI: 1.8\u20138.3) were significant predictors of maternal anaemia. The relatively high prevalence of SP resistance markers indicates sustained drug pressure in this setting. Although SP appears to remain effective for intermittent preventive treatment in pregnancy, continued molecular surveillance is warranted to inform malaria control policies.\n                  <\/jats:p>","DOI":"10.1051\/parasite\/2026035","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T07:53:01Z","timestamp":1782201181000},"page":"36","source":"Crossref","is-referenced-by-count":0,"title":["Molecular surveillance of\n                    <i>Plasmodium falciparum<\/i>\n                    resistance to sulfadoxine-pyrimethamine among pregnant women attending antenatal clinics in Bobo-Dioulasso, Burkina Faso"],"prefix":"10.1051","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8512-8561","authenticated-orcid":false,"given":"Mamoudou","family":"Ciss\u00e9","sequence":"first","affiliation":[{"name":"Laboratoire de recherche, Centre MURAZ","place":["Burkina Faso"]},{"name":"Institut Sup\u00e9rieur des Sciences de la Sant\u00e9, Universit\u00e9 Nazi BONI","place":["Burkina Faso"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alamissa","family":"Soulama","sequence":"additional","affiliation":[{"name":"Laboratoire de recherche, Centre MURAZ","place":["Burkina Faso"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edmond W.","family":"Silga","sequence":"additional","affiliation":[{"name":"Laboratoire de recherche, Centre MURAZ","place":["Burkina Faso"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel K.","family":"Gomgnimbou","sequence":"additional","affiliation":[{"name":"Laboratoire de recherche, Centre MURAZ","place":["Burkina Faso"]},{"name":"Institut Sup\u00e9rieur des Sciences de la Sant\u00e9, Universit\u00e9 Nazi BONI","place":["Burkina Faso"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2026,6,22]]},"reference":[{"key":"R1","first-page":"105","volume":"14","author":"Bamba","year":"2013","journal-title":"Pan African Medical Journal"},{"key":"R2","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1186\/s12936-015-0909-7","volume":"14","author":"Braunm","year":"2015","journal-title":"Malaria Journal"},{"key":"R3","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s12936-017-1695-1","volume":"16","author":"Ciss\u00e9","year":"2017","journal-title":"Malaria Journal"},{"key":"R4","first-page":"3","volume":"3","author":"Ciss\u00e9","year":"2025","journal-title":"African Journal of Parasitology, Mycology and Entomology"},{"key":"R5","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/1475-2875-13-41","volume":"13","author":"Coulibaly","year":"2014","journal-title":"Malaria Journal"},{"key":"R6","doi-asserted-by":"crossref","first-page":"e39","DOI":"10.1017\/S0950268824000177","volume":"152","author":"Das","year":"2024","journal-title":"Epidemiology and Infection"},{"key":"R7","first-page":"13","volume":"2","author":"Diouf","year":"2024","journal-title":"African Journal of Parasitology, Mycology and Entomology"},{"key":"R8","first-page":"100569","volume":"26","author":"Djontu","year":"2024","journal-title":"International Journal for Parasitology: Drugs and Drug Resistance"},{"key":"R9","doi-asserted-by":"crossref","first-page":"e0271489","DOI":"10.1371\/journal.pone.0271489","volume":"17","author":"Dosoo","year":"2022","journal-title":"PLoS One"},{"key":"R10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/expr.1998.4274","volume":"89","author":"Duraisingh","year":"1998","journal-title":"Experimental Parasitology"},{"key":"R11","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1111\/j.1365-3156.2008.02215.x","volume":"14","author":"Gies","year":"2009","journal-title":"Tropical Medicine and International Health"},{"key":"R12","doi-asserted-by":"crossref","first-page":"e1001788","DOI":"10.1371\/journal.pmed.1001788","volume":"12","author":"Hofmann","year":"2015","journal-title":"PLoS Medicine"},{"key":"R13","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.trstmh.2008.01.003","volume":"102","author":"Hyde","year":"2008","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"R14","doi-asserted-by":"crossref","first-page":"e0007223","DOI":"10.1371\/journal.pntd.0007223","volume":"13","author":"Juma","year":"2019","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"R15","doi-asserted-by":"crossref","first-page":"996","DOI":"10.4269\/ajtmh.2010.10-0249","volume":"83","author":"Leke","year":"2010","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"R16","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1093\/cid\/ciq049","volume":"52","author":"Men\u00e9ndez","year":"2011","journal-title":"Clinical Infectious Diseases"},{"key":"R17","unstructured":"Minist\u00e8re de la sant\u00e9. 2014. 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