{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:22:49Z","timestamp":1786116169270,"version":"build-2736575974"},"reference-count":64,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":217,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["GACR 21-23773S"],"award-info":[{"award-number":["GACR 21-23773S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015829","name":"Ministerstwo Zdrowia","doi-asserted-by":"publisher","award":["SUBZ.A060.25.102"],"award-info":[{"award-number":["SUBZ.A060.25.102"]}],"id":[{"id":"10.13039\/501100015829","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2026,7,6]]},"published-print":{"date-parts":[[2026]]},"abstract":"<jats:p>\n                    The European brown hare (\n                    <jats:italic>Lepus europaeus<\/jats:italic>\n                    ) is an ecologically important small game mammal whose populations have declined in many European countries. A total of 370 faecal samples collected between 2022 and 2024 from hares in the Czechia and Slovakia were molecularly screened for the presence of\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    spp. (18S rRNA,\n                    <jats:italic>gp60<\/jats:italic>\n                    ),\n                    <jats:italic>Giardia intestinalis<\/jats:italic>\n                    (\n                    <jats:italic>TPI<\/jats:italic>\n                    ),\n                    <jats:italic>Encephalitozoon<\/jats:italic>\n                    spp., and\n                    <jats:italic>Enterocytozoon bieneusi<\/jats:italic>\n                    (ITS). qPCR analysis confirmed the presence of\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    spp. (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a015; 4.1%; CI: 2.5\u20136.6%),\n                    <jats:italic>Encephalitozoon<\/jats:italic>\n                    spp. (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a034; 9.2%; CI: 6.7\u201312.6%), and\n                    <jats:italic>E. bieneusi<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a016; 4.3%; CI: 2.6\u20136.9%). Sequence analyses revealed the presence of\n                    <jats:italic>C. parvum<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a02),\n                    <jats:italic>C. sciurinum<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a03),\n                    <jats:italic>C. ubiquitum<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a05), and\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    sp. deer mouse genotype IV (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a05). At the\n                    <jats:italic>gp60<\/jats:italic>\n                    locus,\n                    <jats:italic>C. parvum<\/jats:italic>\n                    belonged to subtypes IIaA15G1R1 and IIaA23G1R1,\n                    <jats:italic>C. sciurinum<\/jats:italic>\n                    to subtypes VIIIcA10G2R1 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a02) and VIIIbA11G1R1 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a01), and\n                    <jats:italic>C. ubiquitum<\/jats:italic>\n                    to subtypes XIIa (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a03) and XIId (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a02). Five\n                    <jats:italic>E. bieneusi<\/jats:italic>\n                    genotypes were identified: D (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a08), CHALT1 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a01), WildBoar3 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a01), CHN-F1 (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a02), and C (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a04).\n                    <jats:italic>Encephalitozoon<\/jats:italic>\n                    sequencing revealed\n                    <jats:italic>E. cuniculi<\/jats:italic>\n                    genotypes I (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a06), II (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a026), and III (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a01), and\n                    <jats:italic>E. hellem<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a01).\n                    <jats:italic>Giardia intestinalis<\/jats:italic>\n                    was not detected.\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    sp. deer mouse genotype IV is reported here for the first time in Europe.\n                  <\/jats:p>","DOI":"10.1051\/parasite\/2026042","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T08:08:35Z","timestamp":1786003715000},"page":"43","source":"Crossref","is-referenced-by-count":0,"title":["Genetic characterisation of\n                    <i>Cryptosporidium<\/i>\n                    spp.,\n                    <i>Encephalitozoon<\/i>\n                    spp., and\n                    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