{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:06:47Z","timestamp":1789387607189,"version":"build-2803163510"},"reference-count":67,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"vor","delay-in-days":256,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82372276"],"award-info":[{"award-number":["82372276"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2026,8,24]]},"published-print":{"date-parts":[[2026]]},"abstract":"<jats:p>\n                    The aim of the present study was to characterize the molecular properties of a novel\n                    <jats:italic>Trichinella spiralis<\/jats:italic>\n                    aspartic protease (TsEasp) and to investigate its role in the larval invasion of host intestinal epithelial cells. The full-length TsEasp cDNA was cloned and expressed, and recombinant TsEasp protein (rTsEasp) was purified. Immunization of mice with rTsEasp elicited a robust specific IgG response. qPCR analyses revealed that TsEasp was transcribed at all\n                    <jats:italic>T. spiralis<\/jats:italic>\n                    developmental stages, with markedly higher transcription levels in intestinal infective larvae and adult worms. Indirect immunofluorescence testing showed that TsEasp was expressed throughout the\n                    <jats:italic>T. spiralis<\/jats:italic>\n                    life cycle, and mainly localized at the cuticle, and embryonic periphery in adult females. Far-western blot and indirect immunofluorescence confirmed that rTsEasp binds specifically to Caco-2 cells, predominantly on the cell membrane. The\n                    <jats:italic>in vitro<\/jats:italic>\n                    invasion assay showed that rTsEasp significantly accelerated the larval invasion of Caco-2 monolayers in a dose-dependent manner of rTsEasp protein, whereas anti-rTsEasp antibodies and RNAi markedly suppressed this invasion. Collectively, our results indicate that TsEasp is a key invasive virulence factor in\n                    <jats:italic>T. spiralis<\/jats:italic>\n                    infection.\n                  <\/jats:p>","DOI":"10.1051\/parasite\/2026050","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:45:56Z","timestamp":1789386356000},"page":"51","source":"Crossref","is-referenced-by-count":0,"title":["Molecular characterization of a novel\n                    <i>Trichinella spiralis<\/i>\n                    aspartyl protease and its role in the larval invasion of host intestinal epithelial cells"],"prefix":"10.1051","volume":"33","author":[{"given":"Yao","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ru","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin Zhuo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao Rong","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruo Dan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong Quan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8726-5498","authenticated-orcid":false,"given":"Jing","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University","place":["PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"106112","DOI":"10.1016\/j.actatropica.2021.106112","volume":"224","author":"Bai","year":"2021","journal-title":"Acta Tropica"},{"issue":"2","key":"R2","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.gene.2012.03.062","volume":"500","author":"Balasubramanian","year":"2012","journal-title":"Gene"},{"key":"R3","doi-asserted-by":"crossref","unstructured":"Barrett AJ. 2001. 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