{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T08:50:44Z","timestamp":1791449444116,"version":"4.3.3"},"reference-count":56,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":280,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"National Reference Center for Echinococcoses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2026,9,22]]},"published-print":{"date-parts":[[2026]]},"abstract":"<jats:p>\n                    Genotyping of cystic echinococcosis (CE) specimens enables the identification of predominant species and genotypes, yielding critical insights into disease epidemiology. Species identification is essential for the development of effective prevention and control strategies. In this context, we assessed the molecular diversity of\n                    <jats:italic>E. granulosus<\/jats:italic>\n                    sensu lato among surgically confirmed human CE cases recruited from Algerian referral hospitals. Hydatid cysts were collected from 31 patients undergoing surgery for pulmonary and\/or hepatic CE in Algeria between 2022 and 2024. Hydatid fluid, cyst wall, and laminated-layer preparations were used for DNA extraction. End-point PCR coupled with Sanger sequencing, targeting the mitochondrial 12S-\n                    <jats:italic>rrnS<\/jats:italic>\n                    ,\n                    <jats:italic>nad5<\/jats:italic>\n                    , and\n                    <jats:italic>nad2<\/jats:italic>\n                    markers, was performed for species identification and genotyping, with real-time PCR (qPCR) performed in parallel. End-point PCR was successful in 29 cases (93.5%), while qPCR detected DNA of\n                    <jats:italic>Echinococcus<\/jats:italic>\n                    spp. in 30 cases (96.8%). The additional qPCR-positive sample showed late amplification but could not be assigned to a genotype because no sequence was obtained. Among the 29 molecularly characterized samples,\n                    <jats:italic>E. granulosus<\/jats:italic>\n                    sensu stricto genotype G1 was predominant and was identified in 27 samples (93.1%). One isolate was identified as\n                    <jats:italic>E. granulosus<\/jats:italic>\n                    sensu stricto genotype G3, and one as\n                    <jats:italic>E. canadensis<\/jats:italic>\n                    G6\/G7. Genotype G1 and G3 cases were distributed in northern and central Algeria, while the G6\/G7 isolate was detected in a patient from Tindouf, southern Algeria. These results demonstrate the predominance of\n                    <jats:italic>E. granulosus<\/jats:italic>\n                    sensu stricto genotype G1 among the molecularly characterized human cystic echinococcosis cases in this series.\n                  <\/jats:p>","DOI":"10.1051\/parasite\/2026058","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T08:00:46Z","timestamp":1791446446000},"page":"59","source":"Crossref","is-referenced-by-count":0,"title":["Molecular characterization of\n                    <i>Echinococcus<\/i>\n                    species causing human cystic echinococcosis in Algeria: predominance of\n                    <i>Echinococcus granulosus<\/i>\n                    sensu stricto"],"prefix":"10.1051","volume":"33","author":[{"given":"Rania Sihem","family":"Boussa","sequence":"first","affiliation":[{"name":"Team Cytokines and NOSynthases: Immunity and Pathogeny, LBCM; FSB, USTHB","place":["Algeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Perrine","family":"Duquet","sequence":"additional","affiliation":[{"name":"National Reference Centre for Echinococcoses, Department of Parasitology-Mycology University Hospital of Besan\u00e7on","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coralie","family":"Barrera","sequence":"additional","affiliation":[{"name":"National Reference Centre for Echinococcoses, Department of Parasitology-Mycology University Hospital of Besan\u00e7on","place":["France"]},{"name":"Chrono-Environnement Laboratory UMR\/CNRS 6249, Universit\u00e9 Marie et Louis Pasteur, Universit\u00e9 Marie et Louis Pasteur","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise","family":"Roy","sequence":"additional","affiliation":[{"name":"Chrono-Environnement Laboratory UMR\/CNRS 6249, Universit\u00e9 Marie et Louis Pasteur, Universit\u00e9 Marie et Louis Pasteur","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manel","family":"Amri","sequence":"additional","affiliation":[{"name":"Team Cytokines and NOSynthases: Immunity and Pathogeny, LBCM; FSB, USTHB","place":["Algeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imene","family":"Soufli","sequence":"additional","affiliation":[{"name":"Team Cytokines and NOSynthases: Immunity and Pathogeny, LBCM; FSB, USTHB","place":["Algeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houda","family":"Belguendouz","sequence":"additional","affiliation":[{"name":"Team Cytokines and NOSynthases: Immunity and Pathogeny, LBCM; FSB, USTHB","place":["Algeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nassim","family":"Sid-Idris","sequence":"additional","affiliation":[{"name":"Department of Surgery, Djillali Belkhenchir Hospital","place":["Algeria"]},{"name":"University of Health Sciences Youcef El Khatib","place":["Algeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chafia","family":"Touil-Boukoffa","sequence":"additional","affiliation":[{"name":"Algerian Academy of Sciences and Technologies","place":["Algeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Millon","sequence":"additional","affiliation":[{"name":"National Reference Centre for Echinococcoses, Department of Parasitology-Mycology University Hospital of Besan\u00e7on","place":["France"]},{"name":"Chrono-Environnement Laboratory UMR\/CNRS 6249, Universit\u00e9 Marie et Louis Pasteur, Universit\u00e9 Marie et Louis Pasteur","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karima","family":"Achour","sequence":"additional","affiliation":[{"name":"University of Health Sciences Youcef El Khatib","place":["Algeria"]},{"name":"Algerian Academy of Sciences and Technologies","place":["Algeria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2054-1619","authenticated-orcid":false,"given":"Jenny","family":"Knapp","sequence":"additional","affiliation":[{"name":"National Reference Centre for Echinococcoses, Department of Parasitology-Mycology University Hospital of Besan\u00e7on","place":["France"]},{"name":"Chrono-Environnement Laboratory UMR\/CNRS 6249, Universit\u00e9 Marie et Louis Pasteur, Universit\u00e9 Marie et Louis Pasteur","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"740","DOI":"10.34188\/bjaerv7n1-057","volume":"7","author":"Adili","year":"2024","journal-title":"Brazilian Journal of Animal and Environmental Research"},{"key":"R2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.ijpara.2013.08.008","volume":"44","author":"Alvarez Rojas","year":"2014","journal-title":"International Journal for Parasitology"},{"key":"R3","doi-asserted-by":"crossref","first-page":"e0009253","DOI":"10.1371\/journal.pntd.0009253","volume":"15","author":"Amarir","year":"2021","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"R4","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0304-4017(03)00255-3","volume":"116","author":"Bardonnet","year":"2003","journal-title":"Veterinary Parasitology"},{"key":"R5","first-page":"416","volume":"3","author":"Benchikh El Fegoun","year":"2023","journal-title":"R\u00e9sultats pr\u00e9liminaires. M\u00e9decine Tropicale et Sant\u00e9 Internationale"},{"key":"R6","doi-asserted-by":"crossref","first-page":"107957","DOI":"10.1016\/j.actatropica.2025.107957","volume":"273","author":"Benmarce","year":"2026","journal-title":"Acta Tropica"},{"key":"R7","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1186\/s13071-024-06581-2","volume":"17","author":"Borhani","year":"2024","journal-title":"Parasites & Vectors"},{"key":"R8","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.exppara.2016.12.016","volume":"173","author":"Bou\u00e9","year":"2017","journal-title":"Experimental Parasitology"},{"key":"R9","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1093\/trstmh\/tru138","volume":"108","author":"Boufana","year":"2014","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"R10","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1186\/s13071-022-05197-8","volume":"15","author":"Casulli","year":"2022","journal-title":"Parasites & Vectors"},{"key":"R11","doi-asserted-by":"crossref","unstructured":"\u00c7elik F, Sel\u00e7uk M, Kilinc S, Kesik H, Ahmed H, Wang Y, \u015eim\u015fek S, Cao J. 2024. Molecular discrimination of G1 and G3 genotypes of Echinococcus granulosus sensu stricto obtained from human, cattle, and sheep using the mitochondrial NADH dehydrogenase subunit 5 marker. Acta Tropica, 107124.","DOI":"10.1016\/j.actatropica.2024.107124"},{"key":"R12","doi-asserted-by":"crossref","unstructured":"De la Cruz-Saldana T, Bustos JA, Requena-Herrera MP, Martinez-Merizalde N, Ortiz-Cam L, C\u00e1ceres AL, Guzman C, Gavidia CM, Ugarte-Gil C, Castillo-Neyra R. 2025. A scoping review on control strategies for Echinococcus granulosus sensu lato. IJID One Health, 100078.","DOI":"10.1016\/j.ijidoh.2025.100078"},{"key":"R13","doi-asserted-by":"crossref","first-page":"618","DOI":"10.3389\/fvets.2020.00618","volume":"7","author":"Dehghani","year":"2020","journal-title":"Frontiers in Veterinary Science"},{"key":"R14","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/bs.apar.2016.11.001","volume":"95","author":"Deplazes","year":"2017","journal-title":"Advances in Parasitology"},{"key":"R15","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/j.ijpara.2003.12.013","volume":"34","author":"Dinkel","year":"2004","journal-title":"International Journal for Parasitology"},{"key":"R16","doi-asserted-by":"crossref","first-page":"130","DOI":"10.3166\/bspe-2020-0130","volume":"113","author":"El Fegoun","year":"2020","journal-title":"Bulletin de la Soci\u00e9t\u00e9 de Pathologie Exotique"},{"key":"R17","first-page":"107","volume":"13","author":"El-Sherbini","year":"2020","journal-title":"Egyptian Journal of Pathology"},{"key":"R18","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1051\/parasite\/2024029","volume":"31","author":"Gharbi","year":"2024","journal-title":"Parasite"},{"key":"R19","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1017\/S003118202300046X","volume":"150","author":"Gon\u00e7alves Baptista","year":"2023","journal-title":"Parasitology"},{"key":"R20","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.3748\/wjg.v18.i13.1425","volume":"18","author":"Grosso","year":"2012","journal-title":"World Journal of Gastroenterology"},{"key":"R21","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s12595-009-0010-8","volume":"62","author":"Gudewar","year":"2009","journal-title":"Proceedings of the Zoological Society"},{"key":"R22","first-page":"95","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symposium Series"},{"issue":"85\u201386","key":"R23","first-page":"112","volume":"1\u20132","author":"Hamrat","year":"2013","journal-title":"Agricultura \u2013 \u015etiin\u0163\u0103 \u015fi practic\u0103"},{"key":"R24","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1186\/s13071-022-05598-9","volume":"15","author":"Hod\u017ei\u0107","year":"2022","journal-title":"Parasites & Vectors"},{"key":"R25","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1006\/expr.2000.4518","volume":"95","author":"Kamenetzky","year":"2000","journal-title":"Experimental Parasitology"},{"key":"R26","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1186\/s13071-022-05474-6","volume":"15","author":"Karshima","year":"2022","journal-title":"Parasites & Vectors"},{"key":"R27","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s00436-024-08176-3","volume":"123","author":"Kheninef","year":"2024","journal-title":"Parasitology Research"},{"key":"R28","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.meegid.2017.04.023","volume":"52","author":"Kinkar","year":"2017","journal-title":"Infection, Genetics and Evolution"},{"key":"R29","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.meegid.2018.06.026","volume":"64","author":"Kinkar","year":"2018","journal-title":"Infection, Genetics and Evolution"},{"key":"R30","doi-asserted-by":"crossref","first-page":"196","DOI":"10.47717\/turkjsurg.2022.5427","volume":"38","author":"K\u0131r\u0131\u015f","year":"2022","journal-title":"Turkish Journal of Surgery"},{"key":"R31","doi-asserted-by":"crossref","first-page":"2950","DOI":"10.1128\/AEM.03467-15","volume":"82","author":"Knapp","year":"2016","journal-title":"Applied and Environmental Microbiology"},{"key":"R32","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1051\/parasite\/2023003","volume":"30","author":"Knapp","year":"2023","journal-title":"Parasite"},{"key":"R33","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s00436-018-6118-x","volume":"118","author":"Laatamna","year":"2019","journal-title":"Parasitology Research"},{"key":"R34","first-page":"337","volume":"67","author":"Laatamna","year":"2021","journal-title":"Annals of Parasitology"},{"key":"R35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.26633\/RPSP.2025.13","volume":"49","author":"Larrieu","year":"2025","journal-title":"Revista Panamericana de Salud P\u00fablica"},{"key":"R36","doi-asserted-by":"crossref","first-page":"103941","DOI":"10.1016\/j.meegid.2019.103941","volume":"74","author":"Laurim\u00e4e","year":"2019","journal-title":"Infection, Genetics and Evolution"},{"key":"R37","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1007\/s00436-006-0286-9","volume":"100","author":"Maillard","year":"2007","journal-title":"Parasitology Research"},{"key":"R38","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.vetpar.2010.09.014","volume":"175","author":"Maillard","year":"2011","journal-title":"Veterinary Parasitology"},{"key":"R39","doi-asserted-by":"crossref","first-page":"3195","DOI":"10.1007\/s00436-021-07223-7","volume":"120","author":"Moussa","year":"2021","journal-title":"Parasitology Research"},{"key":"R40","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.exppara.2017.10.002","volume":"183","author":"Nikmanesh","year":"2017","journal-title":"Experimental Parasitology"},{"key":"R41","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s13071-024-06184-x","volume":"17","author":"Nocerino","year":"2024","journal-title":"Parasites & Vectors"},{"key":"R42","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.1111\/tbed.13571","volume":"67","author":"Ohiolei","year":"2020","journal-title":"Transboundary and Emerging Diseases"},{"key":"R43","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.exppara.2015.12.010","volume":"161","author":"Roelfsema","year":"2016","journal-title":"Experimental Parasitology"},{"key":"R44","doi-asserted-by":"crossref","first-page":"106616","DOI":"10.1016\/j.actatropica.2022.106616","volume":"234","author":"Samari","year":"2022","journal-title":"Acta Tropica"},{"key":"R45","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1007\/s11033-019-04625-w","volume":"46","author":"Santucciu","year":"2019","journal-title":"Molecular Biology Reports"},{"key":"R46","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1186\/s13071-021-04869-1","volume":"14","author":"Shahabi","year":"2021","journal-title":"Parasites & Vectors"},{"key":"R47","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1007\/s12639-022-01467-5","volume":"46","author":"Tahiri","year":"2022","journal-title":"Journal of Parasitic Diseases"},{"key":"R48","doi-asserted-by":"crossref","first-page":"453","DOI":"10.3390\/pathogens9060453","volume":"9","author":"Thompson","year":"2020","journal-title":"Pathogens"},{"key":"R49","doi-asserted-by":"crossref","first-page":"102810","DOI":"10.1016\/j.jiph.2025.102810","volume":"18","author":"Tuergan","year":"2025","journal-title":"Journal of Infection and Public Health"},{"key":"R50","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1051\/parasite\/2020024","volume":"27","author":"Vuitton","year":"2020","journal-title":"Parasite"},{"key":"R51","first-page":"5","volume":"3","author":"Wang","year":"2023","journal-title":"One Health and Zoonoses"},{"key":"R52","doi-asserted-by":"crossref","first-page":"e00075-18","DOI":"10.1128\/CMR.00075-18","volume":"32","author":"Wen","year":"2019","journal-title":"Clinical Microbiology Reviews"},{"key":"R53","doi-asserted-by":"crossref","first-page":"e0010568","DOI":"10.1371\/journal.pntd.0010568","volume":"16","author":"Widdicombe","year":"2022","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"R54","doi-asserted-by":"crossref","first-page":"04056","DOI":"10.7189\/jogh.14.04056","volume":"14","author":"Yang","year":"2024","journal-title":"Journal of Global Health"},{"key":"R55","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.1007\/s00436-016-4994-5","volume":"115","author":"Zait","year":"2016","journal-title":"Parasitology Research"},{"key":"R56","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.actatropica.2017.03.034","volume":"171","author":"Zeghir-Bouteldja","year":"2017","journal-title":"Acta Tropica"}],"container-title":["Parasite"],"original-title":[],"link":[{"URL":"https:\/\/www.parasite-journal.org\/10.1051\/parasite\/2026058\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T08:00:55Z","timestamp":1791446455000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.parasite-journal.org\/10.1051\/parasite\/2026058"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":56,"alternative-id":["parasite260036"],"URL":"https:\/\/doi.org\/10.1051\/parasite\/2026058","relation":{},"ISSN":["1776-1042"],"issn-type":[{"value":"1776-1042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}