{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T08:21:41Z","timestamp":1765268501137,"version":"build-2065373602"},"reference-count":64,"publisher":"EDP Sciences","issue":"1","license":[{"start":{"date-parts":[[2018,8,7]],"date-time":"2018-08-07T00:00:00Z","timestamp":1533600000000},"content-version":"vor","delay-in-days":218,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ci\u00eancia T\u00e9c. Vitiv."],"accepted":{"date-parts":[[2018,7,9]]},"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>This study aims to compare the leaf morphoanatomical characteristics of seven<jats:italic>Vitis vinifera<\/jats:italic>subsp.<jats:italic>vinifera<\/jats:italic>white cultivars with different geographical origin, grown side by side at the same \u201cterroir\u201d. The leaf morphoanatomical traits analyzed under light and scanning electron microscopy (SEM) showed large variability among genotypes. Significant differences between cultivars were observed in single leaf area, specific leaf area, leaf density and in the thickness of cuticle, upper and lower epidermal cells and mesophyll. Leaf dry mass per area presented a significant positive correlation with leaf density but showed no correlation with leaf thickness, results that can be explained by the quite different mesophyll structure, epidermal cells and cuticle thickness. \u2018Alvarinho\u2019, \u2018Encruzado\u2019, \u2018Macabeu\u2019 and \u2018Viosinho\u2019 were the cultivars with the highest thickness of cuticle and mesophyll tissues. Under SEM magnification three types of stomata were identified: sunken, at the same level and raised above, with the same level type presenting the higher percentage in all cultivars. Stomata density presented significant differences between cultivars, with \u2018Macabeu\u2019 showing the highest value and \u2018Alvarinho\u2019 and \u2018Arinto\u2019 the lowest ones. The hairs on the lower surface presented a similar woolly aspect in all cultivars. Calcium oxalate crystals, raphids and druses were visible and widely distributed in the parenchyma tissues. The observed differences in leaf traits among genotypes suggest a major role of leaf anatomy in determining grapevine capacity for coping with different environmental conditions.<\/jats:p>","DOI":"10.1051\/ctv\/20183301090","type":"journal-article","created":{"date-parts":[[2018,8,7]],"date-time":"2018-08-07T09:07:24Z","timestamp":1533632844000},"page":"90-101","source":"Crossref","is-referenced-by-count":15,"title":["Leaf morphoanatomy traits in white grapevine cultivars with distinct geographical origin"],"prefix":"10.1051","volume":"33","author":[{"given":"Generosa","family":"Teixeira","sequence":"first","affiliation":[]},{"given":"Ana","family":"Monteiro","sequence":"additional","affiliation":[]},{"given":"Cristina","family":"Santos","sequence":"additional","affiliation":[]},{"given":"Carlos M.","family":"Lopes","sequence":"additional","affiliation":[]}],"member":"250","published-online":{"date-parts":[[2018,8,7]]},"reference":[{"key":"R1","unstructured":"Alleweldt G., Dettweiler-M\u00fcnch E., 1994. The genetic resources of Vitis. world list of grapevine collections. Institut f\u00fcr Rebenz\u00fcchtung Geilweilerhof. 2nd edition."},{"issue":"1","key":"R2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1051\/ctv\/20173201023","volume":"32","author":"Atak","year":"2017","journal-title":"Ci\u00eancia T\u00e9c. Vitiv."},{"issue":"8","key":"R3","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1002\/jemt.20955","volume":"74","author":"Ben Salem-Fnayou","year":"2011","journal-title":"Res. Tech."},{"key":"R4","unstructured":"B\u00f6hm J., 2007. Portugal vit\u00edcola. O grande livro das castas. Chaves Ferreira \u2013 Publica\u00e7\u00f5es S.A., Lisboa. 230 pp."},{"key":"R5","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/S0168-9452(02)00135-8","volume":"163","author":"Bosabalidis","year":"2002","journal-title":"Pl. Sc."},{"key":"R6","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1111\/j.1755-0238.2011.00157.x","volume":"17","author":"Boso","year":"2011a","journal-title":"Aust. J. Grape Wine Res."},{"key":"R7","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.cropro.2014.04.018","volume":"63","author":"Boso","year":"2014","journal-title":"Crop Prot."},{"key":"R8","first-page":"43","volume":"49","author":"Boso","year":"2010","journal-title":"Vitis"},{"key":"R9","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.scienta.2011.01.022","volume":"128","author":"Boso","year":"2011b","journal-title":"Sci. Hort."},{"key":"R10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1365-3040.1997.d01-15.x","volume":"20","author":"Brewer","year":"1997","journal-title":"Plant Cell Environ"},{"key":"R11","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1469-8137.2007.02351.x","volume":"178","author":"Carson","year":"2008","journal-title":"New Phytologist"},{"key":"R12","doi-asserted-by":"crossref","first-page":"20","DOI":"10.3389\/fenvs.2015.00020","volume":"3","author":"Carvalho","year":"2015","journal-title":"Front. Environ. Sci."},{"key":"R13","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1071\/FP11156","volume":"39","author":"Costa","year":"2012","journal-title":"Funct. Pl. Biol."},{"key":"R14","first-page":"401","volume":"17","author":"Denisov","year":"1970","journal-title":"Sb. Tr. Asp."},{"key":"R15","unstructured":"Dickison W.C., 2000. Integrative plant anatomy. Harcourt Academic Press. San Diego."},{"issue":"1588","key":"R16","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1098\/rstb.2011.0272","volume":"367","author":"Doheny-Adams","year":"2012","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"R17","first-page":"91","volume":"19","author":"D\u00fcring","year":"1980","journal-title":"Vitis"},{"key":"R18","doi-asserted-by":"crossref","unstructured":"Esau K., 1977. Anatomy of seed plants. 2nd ed. John Wiley & Sons, New York.","DOI":"10.2307\/2418500"},{"key":"R19","unstructured":"Eurostat, 2016. Agri-environmental indicator - consumption of pesticides. Eurostat statistics explains. http:\/\/ec.europa.eu\/eurostat\/statistics-explained\/index.php\/Agrienvironmental_indicator_-_consumption_of_pesticides"},{"issue":"13","key":"R20","doi-asserted-by":"crossref","first-page":"3965","DOI":"10.1093\/jxb\/ert319","volume":"64","author":"Flexas","year":"2013","journal-title":"J. Exp. Bot."},{"key":"R21","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1139\/b94-222","volume":"72","author":"Grammatikopoulous","year":"1994","journal-title":"Can. J. Bot."},{"key":"R22","first-page":"131","volume":"37","author":"G\u00f3mez-del-Campo","year":"2003","journal-title":"J. Int. Sci. Vigne Vin"},{"key":"R23","unstructured":"Hayat M., 1981. Principles and techniques of electron microscopy. Biological Applications. 2nd ed. Ed. Arnold Publ. London."},{"key":"R24","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.tplants.2013.11.002","volume":"19","author":"He","year":"2014","journal-title":"Trends Plant Sci."},{"key":"R25","first-page":"225","volume":"20","author":"Heged\u00fcs","year":"1974","journal-title":"Acta Bot Acad Sci Hung"},{"key":"R26","unstructured":"IFV, 2007 Catalogue des vari\u00e9t\u00e9s et clones de vigne cultiv\u00e9s en France. Institute Fran\u00e7ais de la Vigne et du Vin, Minist\u00e8re de l\u2019Agriculture et da la P\u00eache \u2013 CTPS, 2\u00e8me edition. Montpellier, France."},{"key":"R27","unstructured":"IPCC, 2013: Climate change 2013: The physical science basis. Contribution of working group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change [Stocker, T.F., D. Qin, G.-K. Plattner, M. Tignor, S.K. Allen, J. Boschung, A. Nauels, Y. Xia, V. Bex and P.M. Midgley (eds.)]. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA, 1535 pp."},{"key":"R28","unstructured":"IVV, 2017 Lista de castas. Instituto da Vinha e do Vinho, Minist\u00e9rio da Agricultura, Florestas e Desenvolvimento Rural. http:\/\/www.ivv.gov.pt\/np4\/111\/, Accessed at 8 December 2017"},{"key":"R29","doi-asserted-by":"crossref","unstructured":"Jones H.G., 2014. Plants and microclimate: A quantitative approach to environmental plant physiology. Cap. 10, Drought and other abiotic stresses, p. 255-289. 3rd Ed., Cambridge University Press, Cambridge.","DOI":"10.1017\/CBO9780511845727.011"},{"key":"R30","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1139\/b95-039","volume":"73","author":"Karabourniotis","year":"1995","journal-title":"Can. J. Bot."},{"key":"R31","unstructured":"Keller M., 2010. The science of grapevines. Anatomy and physiology. Academic Press\/Elsevier. 1st edition, Burlington, MA. 377 p."},{"key":"R32","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1590\/1809-43921977074473","volume":"7","author":"Lleras","year":"1997","journal-title":"Acta Amaz."},{"key":"R33","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1071\/FP09191","volume":"37","author":"Lovisolo","year":"2010","journal-title":"Funct. Plant Biol."},{"key":"R34","unstructured":"Martin T.J., Juniper B.E. 1970. The cuticles of plants. Edward Arnold, London, 347 pp."},{"key":"R35","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1590\/1809-43921980103463","volume":"10","author":"Medri","year":"1980","journal-title":"Arg. Acta Amaz."},{"key":"R36","unstructured":"Metcalfe C., Chalk L., 1979. Anatomy of Dicotyledons, Vol I. 2nd ed. Oxford Univ. Press."},{"key":"R37","first-page":"19","volume":"28","author":"Monteiro","year":"2013","journal-title":"Ci\u00eancia Tec. Vitiv."},{"key":"R38","doi-asserted-by":"crossref","first-page":"41","DOI":"10.17660\/ActaHortic.2016.1115.7","volume":"1115","author":"Montoro","year":"2016","journal-title":"Acta Hort."},{"key":"R39","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1111\/1365-3040.ep11611750","volume":"5","author":"Mott","year":"1982","journal-title":"Plant Cell Environ."},{"key":"R40","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/S0168-9452(03)00120-1","volume":"164","author":"Nakata","year":"2003","journal-title":"Plant Sci."},{"key":"R41","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1046\/j.1469-8137.1999.00466.x","volume":"144","author":"Niinemets","year":"1999","journal-title":"New Physiologist"},{"key":"R42","doi-asserted-by":"crossref","first-page":"2433","DOI":"10.1093\/jxb\/erp045","volume":"60","author":"Niinemets","year":"2009","journal-title":"J. Exp. Bot."},{"issue":"6","key":"R43","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1071\/FP13271","volume":"41","author":"Palliotti","year":"2014","journal-title":"Funct. Plant Biol."},{"key":"R44","doi-asserted-by":"crossref","first-page":"367","DOI":"10.2307\/2259142","volume":"66","author":"Parkhurst","year":"1978","journal-title":"J. Ecology"},{"key":"R45","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0304-4238(02)00201-7","volume":"97","author":"Patakas","year":"2003","journal-title":"Sc. Hort."},{"key":"R46","first-page":"26","volume":"1","author":"Periyanayagam","year":"2013","journal-title":"Innovare Journal of Health Sciences"},{"key":"R47","doi-asserted-by":"crossref","unstructured":"Pertot I., Caffi T., Rossi V., Mugnai L., Hoffmann C., Grando M.S., Gary C., Lafond D., Duso C., Thiery D., Mazzoni V., Anfora G., 2016. A critical review of plant protection tools for reducing pesticide use on grapevine and new perspectives for the implementation of IPM in viticulture. Crop Prot., 1\u201315.","DOI":"10.1016\/j.cropro.2016.11.025"},{"key":"R48","doi-asserted-by":"crossref","first-page":"131","DOI":"10.5344\/ajev.1974.25.3.131","volume":"25","author":"Pratt","year":"1974","journal-title":"Am. J. Enol. Vitic."},{"key":"R49","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1006\/anbo.1999.0975","volume":"84","author":"Prychid","year":"1999","journal-title":"Ann. Bot."},{"key":"R50","unstructured":"Ruzin S.E., 1999. Plant microtechnique and microscopy. Oxford University Press. 322 p."},{"key":"R51","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.agrformet.2012.06.005","volume":"165","author":"Sadras","year":"2012","journal-title":"Agr. For. Meteorol."},{"key":"R52","first-page":"1","volume":"216","author":"Salisbury","year":"1927","journal-title":"Philosophical Transactions of the Royal Society of London"},{"key":"R53","unstructured":"Santa Maria F.C.S., Marcide J.M.O., Organero G.M., Torres I.R., Barba A.B., Rubio de Miguel C., Mu\u00f1oz S.C., S\u00e1iz R.S., 2011. Variedades de vid en Espa\u00f1a. Instituto Madrile\u00f1o de Investigaci\u00f3n y Desarrollo, Agrario y Alimentario. Editorial Agricola Espa\u00f1ola, S.A. Madrid. 489 pp."},{"key":"R54","doi-asserted-by":"crossref","first-page":"177","DOI":"10.17660\/ActaHortic.2017.1157.26","volume":"1157","author":"Serra","year":"2017","journal-title":"Acta Hort."},{"key":"R55","doi-asserted-by":"crossref","first-page":"278","DOI":"10.3732\/ajb.93.2.278","volume":"93","author":"Soejima","year":"2006","journal-title":"Am. J. Bot."},{"key":"R56","first-page":"31","volume":"8","author":"Swanepoel","year":"1987","journal-title":"S. Afr. J. Enol. Vitic."},{"key":"R57","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1104\/pp.110.165472","volume":"155","author":"Terashima","year":"2011","journal-title":"Pl. Physiol."},{"issue":"9","key":"R58","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/j.tig.2006.07.008","volume":"22","author":"This","year":"2006","journal-title":"Trends Genet."},{"issue":"8","key":"R59","doi-asserted-by":"crossref","first-page":"2269","DOI":"10.1093\/jxb\/ert086","volume":"64","author":"Tom\u00e1s","year":"2013","journal-title":"J. Exp. Bot."},{"issue":"2","key":"R60","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1111\/j.1755-0238.2012.00184.x","volume":"18","author":"Tom\u00e1s","year":"2012","journal-title":"Aust. J. Grape. Wine. Res."},{"key":"R61","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1111\/ppl.12180","volume":"152","author":"Tombesi","year":"2014","journal-title":"Physiol. Plant."},{"key":"R62","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.1104\/pp.16.00111","volume":"171","author":"Tooulakou","year":"2016","journal-title":"Plt. Physiol."},{"key":"R63","unstructured":"Wilkinson H.P., 1979. The plant surface (Mainly Leaf). Part I: Stomata. In: Anatomy of Dicotyledons. Vol I. 98\u2013117. 2nd ed. Oxford Univ. Press."},{"key":"R64","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1111\/j.1469-8137.1995.tb03067.x","volume":"131","author":"Woodward","year":"1995","journal-title":"New Phytologist"}],"container-title":["Ci\u00eancia e T\u00e9cnica Vitivin\u00edcola"],"original-title":[],"link":[{"URL":"https:\/\/www.ctv-jve-journal.org\/10.1051\/ctv\/20183301090\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T06:00:53Z","timestamp":1693807253000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ctv-jve-journal.org\/10.1051\/ctv\/20183301090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":64,"journal-issue":{"issue":"1"},"alternative-id":["ctv20183301p90"],"URL":"https:\/\/doi.org\/10.1051\/ctv\/20183301090","relation":{},"ISSN":["2416-3953"],"issn-type":[{"type":"electronic","value":"2416-3953"}],"subject":[],"published":{"date-parts":[[2018]]}}}