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This work aimed to synthetize a MLM-type SL, which consists of triacylglycerols containing a medium-chain FA (M) at <jats:italic>sn<\/jats:italic>-1,3 positions and a long-chain FA (L) at <jats:italic>sn<\/jats:italic>-2 position, by acidolysis of pumpkin seed oil with capric acid, catalyzed by a commercial lipase preparation from <jats:italic>Thermomyces lanuginosa<\/jats:italic> (Lipozyme TL IM). Reactions were performed at 45\u2009\u00b0C, in solvent-free media, at 1:2 molar ratio (pumpkin seed oil:capric acid) and a fixed amount of immobilized lipase of 5%, 10%, 15% or 20%. Incorporations of C10:0 increased with time up to 31\u2009h (29.9\u2009\u00b1\u20090.7\u2009mol-%) when 5% lipase load was used. Significant differences were only observed between the results obtained with 5 and 20% of biocatalyst load. The subsequent experiment was carried out with 5% lipase load, at 45\u2009\u00b0C, 1:2 molar ratio and in the presence of <jats:italic>n<\/jats:italic>-hexane. The results showed slightly higher incorporation yields in the presence of solvent, namely at 48\u2009h-reaction (34.7\u2009\u00b1\u20091.0\u2009mol-%). However, since the structured lipids are to be used in food products, together with environmental and economic concerns, solvent-free systems are preferred. In this study, the synthesis of a MLM-type SL from pumpkin seed oil for food uses was well succeeded.<\/jats:p>","DOI":"10.1051\/ocl\/2018004","type":"journal-article","created":{"date-parts":[[2018,1,31]],"date-time":"2018-01-31T08:51:50Z","timestamp":1517388710000},"page":"A302","source":"Crossref","is-referenced-by-count":10,"title":["Incorporation of capric acid in pumpkin seed oil by <i>sn<\/i>-1,3 regioselective lipase-catalyzed acidolysis"],"prefix":"10.1051","volume":"25","author":[{"given":"Vanessa","family":"Sousa","sequence":"first","affiliation":[]},{"given":"Vitor","family":"Campos","sequence":"additional","affiliation":[]},{"given":"Patr\u00edcia","family":"Nunes","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9567-2165","authenticated-orcid":false,"given":"Paula","family":"Pires-Cabral","sequence":"additional","affiliation":[]}],"member":"250","published-online":{"date-parts":[[2018,1,31]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Abed SM, \nZou X, \nAli AH, \nJin Q, \nWang X. \n2017. \nSynthesis of 1, 3-dioleoyl-2-arachidonoylglycerol-rich structured lipids by lipase-catalyzed acidolysis of microbial oil from Mortierella alpine. \nBioresour Technol. 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