{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:19:44Z","timestamp":1791422384859,"version":"4.3.3"},"reference-count":47,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","gastrojournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Gastroenterology"],"published-print":{"date-parts":[[2015,12]]},"DOI":"10.1053\/j.gastro.2015.08.047","type":"journal-article","created":{"date-parts":[[2015,8,29]],"date-time":"2015-08-29T18:24:41Z","timestamp":1440872681000},"page":"1910-1919.e5","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"title":["Yarrowia lipolytica Lipase 2 Is Stable and Highly Active in Test Meals and Increases Fat Absorption in an Animal Model of Pancreatic Exocrine Insufficiency"],"prefix":"10.1053","volume":"149","author":[{"given":"Ahmed","family":"Aloulou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Schu\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Puccinelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Milano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Delchambre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Leblond","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9","family":"Laugier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Carri\u00e8re","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1053\/j.gastro.2015.08.047_bib1","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/S1542-3565(04)00601-9","article-title":"Quantitative study of digestive enzyme secretion and gastrointestinal lipolysis in chronic pancreatitis","volume":"3","author":"Carri\u00e8re","year":"2005","journal-title":"Clin Gastroenterol Hepatol"},{"key":"10.1053\/j.gastro.2015.08.047_bib2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1136\/pgmj.60.701.225","article-title":"Cystic fibrosis","volume":"60","author":"Hodson","year":"1984","journal-title":"Postgrad Med J"},{"key":"10.1053\/j.gastro.2015.08.047_bib3","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1136\/gut.23.9.777","article-title":"Exocrine pancreatic function tests","volume":"23","author":"Lankisch","year":"1982","journal-title":"Gut"},{"key":"10.1053\/j.gastro.2015.08.047_bib4","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1007\/BF01296724","article-title":"Controversies in the treatment of exocrine pancreatic insufficiency","volume":"27","author":"DiMagno","year":"1982","journal-title":"Dig Dis Sci"},{"key":"10.1053\/j.gastro.2015.08.047_bib5","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1051\/vetres\/2010018","article-title":"Zoonotic hepatitis E: animal reservoirs and emerging risks","volume":"41","author":"Pavio","year":"2010","journal-title":"Vet Res"},{"key":"10.1053\/j.gastro.2015.08.047_bib6","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.3201\/eid2011.140791","article-title":"Foodborne transmission of hepatitis E virus from raw pork liver sausage, France","volume":"20","author":"Renou","year":"2014","journal-title":"Emerg Infect Dis"},{"key":"10.1053\/j.gastro.2015.08.047_bib7","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1097\/MCG.0000000000000113","article-title":"Phylogenetic demonstration of hepatitis E infection transmitted by pork meat ingestion","volume":"49","author":"Riveiro-Barciela","year":"2015","journal-title":"J\u00a0Clin Gastroenterol"},{"key":"10.1053\/j.gastro.2015.08.047_bib8","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1097\/00006676-199205000-00007","article-title":"Therapeutic potential and clinical efficacy of acid-resistant fungal lipase in the treatment of pancreatic steatorrhoea due to cystic fibrosis","volume":"7","author":"Zentler-Munro","year":"1992","journal-title":"Pancreas"},{"key":"10.1053\/j.gastro.2015.08.047_bib9","first-page":"787","article-title":"[Comparison of fungal lipase and pancreatic lipase in exocrine pancreatic insufficiency in man. Study of their in\u00a0vitro properties and intraduodenal bioavailability]","volume":"12","author":"Moreau","year":"1988","journal-title":"Gastroenterol Clin Biol"},{"key":"10.1053\/j.gastro.2015.08.047_bib10","doi-asserted-by":"crossref","first-page":"2048","DOI":"10.1053\/gast.1997.v112.pm9178698","article-title":"Bacterial lipase and high-fat diets in canine exocrine pancreatic insufficiency: a new therapy of steatorrhea?","volume":"112","author":"Suzuki","year":"1997","journal-title":"Gastroenterology"},{"key":"10.1053\/j.gastro.2015.08.047_bib11","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1016\/0016-5085(93)90908-U","article-title":"Secretion and contribution to lipolysis of gastric and pancreatic lipases during a test meal in humans","volume":"105","author":"Carri\u00e8re","year":"1993","journal-title":"Gastroenterology"},{"key":"10.1053\/j.gastro.2015.08.047_bib12","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/0016-5085(94)90081-7","article-title":"Lipolytic activity of bacterial lipase survives better than that of porcine lipase in human gastric and duodenal content","volume":"107","author":"Raimondo","year":"1994","journal-title":"Gastroenterology"},{"key":"10.1053\/j.gastro.2015.08.047_bib13","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/0014-5793(93)81044-Z","article-title":"One-step purification and characterization of human pancreatic lipase expressed in insect cells","volume":"327","author":"Thirstrup","year":"1993","journal-title":"FEBS Lett"},{"key":"10.1053\/j.gastro.2015.08.047_bib14","doi-asserted-by":"crossref","first-page":"2205","DOI":"10.1021\/bi0616089","article-title":"Probing the opening of the pancreatic lipase lid using site-directed spin labeling and EPR spectroscopy","volume":"46","author":"Belle","year":"2007","journal-title":"Biochemistry"},{"key":"10.1053\/j.gastro.2015.08.047_bib15","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1021\/bi801250s","article-title":"Lid opening and unfolding in human pancreatic lipase at low pH revealed by site-directed spin labeling EPR and FTIR spectroscopy","volume":"48","author":"Ranaldi","year":"2009","journal-title":"Biochemistry"},{"key":"10.1053\/j.gastro.2015.08.047_bib16","doi-asserted-by":"crossref","first-page":"5499","DOI":"10.1021\/bi982601x","article-title":"Human pancreatic lipase: colipase dependence and interfacial binding of lid domain mutants","volume":"38","author":"Bezzine","year":"1999","journal-title":"Biochemistry"},{"key":"10.1053\/j.gastro.2015.08.047_bib17","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1159\/000057708","article-title":"Physiological study of pH stability and sensitivity to pepsin of human gastric lipase","volume":"65","author":"Ville","year":"2002","journal-title":"Digestion"},{"key":"10.1053\/j.gastro.2015.08.047_bib18","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1023\/A:1011653220724","article-title":"Large-scale production of a therapeutic protein in transgenic tobacco plants: effect of subcellular targeting on quality of a recombinant dog gastric lipase","volume":"7","author":"Gruber","year":"2001","journal-title":"Mol Breed"},{"key":"10.1053\/j.gastro.2015.08.047_bib19","doi-asserted-by":"crossref","unstructured":"Schmid RD, Verger R. Lipases: interfacial enzymes with attractive applications. Angew Chem Int Ed 1009;37:1608\u20131633.","DOI":"10.1002\/(SICI)1521-3773(19980703)37:12<1608::AID-ANIE1608>3.3.CO;2-M"},{"key":"10.1053\/j.gastro.2015.08.047_bib20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/00006676-200301000-00001","article-title":"Lipase supplementation therapy: standards, alternatives, and perspectives","volume":"26","author":"Layer","year":"2003","journal-title":"Pancreas"},{"key":"10.1053\/j.gastro.2015.08.047_bib21","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/S0016-5085(99)70141-1","article-title":"Effect of bacterial or porcine lipase with low- or high-fat diets on nutrient absorption in pancreatic-insufficient dogs","volume":"116","author":"Suzuki","year":"1999","journal-title":"Gastroenterology"},{"key":"10.1053\/j.gastro.2015.08.047_bib22","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.1016\/j.bbalip.2007.10.006","article-title":"A\u00a0comparative study on two fungal lipases from Thermomyces lanuginosus and Yarrowia lipolytica shows the combined effects of detergents and pH on lipase adsorption and activity","volume":"1771","author":"Aloulou","year":"2007","journal-title":"Biochim Biophys Acta"},{"key":"10.1053\/j.gastro.2015.08.047_bib23","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.bbalip.2006.12.006","article-title":"Purification and biochemical characterization of the LIP2 lipase from Yarrowia lipolytica","volume":"1771","author":"Aloulou","year":"2007","journal-title":"Biochim Biophys Acta"},{"key":"10.1053\/j.gastro.2015.08.047_bib24","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/j.1432-1033.1991.tb16346.x","article-title":"Purification and biochemical characterization of dog gastric lipase","volume":"202","author":"Carri\u00e8re","year":"1991","journal-title":"Eur J Biochem"},{"key":"10.1053\/j.gastro.2015.08.047_bib25","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1021\/bi0518803","article-title":"How gastric lipase, an interfacial enzyme with a Ser-His-Asp catalytic triad, acts optimally at acidic pH","volume":"45","author":"Chahinian","year":"2006","journal-title":"Biochemistry"},{"key":"10.1053\/j.gastro.2015.08.047_bib26","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/0005-2744(78)90232-2","article-title":"An improved large scale procedure for the purification of porcine pancreatic lipase","volume":"525","author":"Rovery","year":"1978","journal-title":"Biochim Biophys Acta"},{"key":"10.1053\/j.gastro.2015.08.047_bib27","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/0006-291X(73)90986-8","article-title":"On porcine pancreatic colipase: large scale purification and some properties","volume":"52","author":"Maylie","year":"1973","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1053\/j.gastro.2015.08.047_bib28","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1053\/gast.2000.18140","article-title":"The specific activities of human digestive lipases measured from the in\u00a0vivo and in\u00a0vitro lipolysis of test meals","volume":"119","author":"Carri\u00e8re","year":"2000","journal-title":"Gastroenterology"},{"key":"10.1053\/j.gastro.2015.08.047_bib29","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s13228-011-0014-5","article-title":"In\u00a0vitro gastrointestinal lipolysis: replacement of human digestive lipases by a combination of rabbit gastric and porcine pancreatic extracts","volume":"2","author":"Capolino","year":"2011","journal-title":"Food Digest"},{"key":"10.1053\/j.gastro.2015.08.047_bib30","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A\u00a0simple method for the isolation and purification of total lipides from animal tissues","volume":"226","author":"Folch","year":"1957","journal-title":"J\u00a0Biol Chem"},{"key":"10.1053\/j.gastro.2015.08.047_bib31","doi-asserted-by":"crossref","first-page":"6543","DOI":"10.1016\/j.chroma.2009.07.061","article-title":"Validation of lipolysis product extraction from aqueous\/biological samples, separation and quantification by thin-layer chromatography with flame ionization detection analysis using O-cholesteryl ethylene glycol as a new internal standard","volume":"1216","author":"Cavalier","year":"2009","journal-title":"J\u00a0Chromatogr A"},{"key":"10.1053\/j.gastro.2015.08.047_bib32","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/S0021-9258(18)57092-7","article-title":"Rapid method for the determination of fat in feces","volume":"177","author":"Van De Kamer","year":"1949","journal-title":"J\u00a0Biol Chem"},{"key":"10.1053\/j.gastro.2015.08.047_bib33","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/0016-5085(86)90695-5","article-title":"Kinetic assay of\u00a0human gastric lipase on short- and long-chain triacylglycerol emulsions","volume":"91","author":"Gargouri","year":"1986","journal-title":"Gastroenterology"},{"key":"10.1053\/j.gastro.2015.08.047_bib34","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1042\/BA20100272","article-title":"A\u00a0highly stable Yarrowia lipolytica lipase formulation for the treatment of pancreatic exocrine insufficiency","volume":"57","author":"Turki","year":"2010","journal-title":"Biotechnol Appl Biochem"},{"key":"10.1053\/j.gastro.2015.08.047_bib35","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1159\/000048838","article-title":"Advantage of expressing the variations in some digestive parameters as a function of gastric emptying instead of time","volume":"64","author":"Carri\u00e8re","year":"2001","journal-title":"Digestion"},{"key":"10.1053\/j.gastro.2015.08.047_bib36","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1002\/ejlt.201200440","article-title":"Biochemical and structural characterization of non-glycosylated Yarrowia lipolytica LIP2 lipase","volume":"115","author":"Aloulou","year":"2013","journal-title":"Eur J Lipid Sci Technol"},{"key":"10.1053\/j.gastro.2015.08.047_bib37","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1111\/j.1365-2036.2007.03563.x","article-title":"In\u00a0vitro comparative study of three pancreatic enzyme preparations: dissolution profiles, active enzyme release and\u00a0acid stability","volume":"27","author":"Aloulou","year":"2008","journal-title":"Aliment Pharmacol Ther"},{"key":"10.1053\/j.gastro.2015.08.047_bib38","doi-asserted-by":"crossref","first-page":"2276","DOI":"10.1038\/ajg.2010.201","article-title":"Pancrelipase delayed-release capsules (CREON) for exocrine pancreatic insufficiency due to chronic pancreatitis or pancreatic surgery: a double-blind randomized trial","volume":"105","author":"Whitcomb","year":"2010","journal-title":"Am J Gastroenterol"},{"key":"10.1053\/j.gastro.2015.08.047_bib39","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1053\/bega.2001.0195","article-title":"Gastric acid suppression and treatment of severe exocrine pancreatic insufficiency","volume":"15","author":"DiMagno","year":"2001","journal-title":"Best Pract Res Clin Gastroenterol"},{"key":"10.1053\/j.gastro.2015.08.047_bib40","doi-asserted-by":"crossref","first-page":"7","DOI":"10.2478\/v10039-009-0011-x","article-title":"The effectiveness of enzymatic replacement therapy measured by turbidimetry and the lipaemic index in exocrine pancreatic insufficient young, growing pigs, fed a high-fat diet","volume":"54","author":"Donaldson","year":"2009","journal-title":"Adv Med Sci"},{"key":"10.1053\/j.gastro.2015.08.047_bib41","series-title":"Chronic Pancreatitis: Novel Concepts in Biology and Therapy","first-page":"445","article-title":"Therapy of pancreatic exocrine insufficiency: new experimental data","author":"Gregory","year":"2002"},{"key":"10.1053\/j.gastro.2015.08.047_bib42","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1046\/j.1439-0396.1999.00238.x","article-title":"Studies on nutrient digestibilities (pre-caecal and total) in pancreatic duct-ligated pigs and the effects of enzyme substitution","volume":"82","author":"Tabeling","year":"1999","journal-title":"J\u00a0Anim Physiol Anim Nutr"},{"key":"10.1053\/j.gastro.2015.08.047_bib43","doi-asserted-by":"crossref","first-page":"2393","DOI":"10.1016\/j.fct.2010.05.077","article-title":"Preliminary safety assessment of Yarrowia lipolytica extracellular lipase: results of acute and 28-day repeated dose oral toxicity studies in rats","volume":"48","author":"Turki","year":"2010","journal-title":"Food Chem Toxicol"},{"key":"10.1053\/j.gastro.2015.08.047_bib44","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1056\/NEJM197304192881603","article-title":"Relations between pancreatic enzyme ouputs and malabsorption in severe pancreatic insufficiency","volume":"288","author":"DiMagno","year":"1973","journal-title":"N\u00a0Engl J Med"},{"key":"10.1053\/j.gastro.2015.08.047_bib45","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1136\/gut.34.7.984","article-title":"Complex carbohydrate malabsorption in exocrine pancreatic insufficiency","volume":"34","author":"Ladas","year":"1993","journal-title":"Gut"},{"key":"10.1053\/j.gastro.2015.08.047_bib46","series-title":"Lipases and Phospholipases in Drug Development","first-page":"195","article-title":"Physiology of gastrointestinal lipolysis and therapeutical use of lipases and digestive lipase inhibitors","author":"Lengsfeld","year":"2004"},{"key":"10.1053\/j.gastro.2015.08.047_bib47","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/S0968-0896(96)00251-9","article-title":"In\u00a0vivo and in\u00a0vitro studies on the stereoselective hydrolysis of tri-\u00a0and diglycerides by gastric and pancreatic lipases","volume":"5","author":"Carri\u00e8re","year":"1997","journal-title":"Bioorg Med Chem"}],"container-title":["Gastroenterology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0016508515012445?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0016508515012445?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:42:44Z","timestamp":1762162964000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0016508515012445"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12]]},"references-count":47,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["S0016508515012445"],"URL":"https:\/\/doi.org\/10.1053\/j.gastro.2015.08.047","relation":{},"ISSN":["0016-5085"],"issn-type":[{"value":"0016-5085","type":"print"}],"subject":[],"published":{"date-parts":[[2015,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Yarrowia lipolytica Lipase 2 Is Stable and Highly Active in Test Meals and Increases Fat Absorption in an Animal Model of Pancreatic Exocrine Insufficiency","name":"articletitle","label":"Article Title"},{"value":"Gastroenterology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1053\/j.gastro.2015.08.047","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 AGA Institute. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}