{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:19:45Z","timestamp":1788236385799,"version":"build-2803163510"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100017618","name":"NIDDK Division of Diabetes Endocrinology and Metabolic Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017618","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008538","name":"PSC Partners Seeking a Cure","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008538","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000997","name":"Arnold and Mabel Beckman Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000997","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","gastrojournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Gastroenterology"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1053\/j.gastro.2026.07.034","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T12:38:22Z","timestamp":1786711102000},"update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Liver Regeneration CRISPR Screen Identifies E3 Ligase NEURL1B Regulates Hepatocyte Mitosis by Destabilizing Microtubule-Organizing Centers"],"prefix":"10.1053","author":[{"given":"Dingzi","family":"Yin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra M.","family":"Vazquez Salgado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunmiao","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amber W.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noam","family":"Erez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"II","given":"Markcus A.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuebo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidemi","family":"Nishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rory L.","family":"Smoot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrienne F.","family":"Gefre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidhi","family":"Jalan-Sakrikar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salah","family":"Adlat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin-Yao","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chady","family":"Meroueh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirk J.","family":"Wangensteen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1053\/j.gastro.2026.07.034_bib1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1038\/s41575-020-0342-4","article-title":"Liver regeneration: biological and pathological mechanisms and implications","volume":"18","author":"Michalopoulos","year":"2021","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"10.1053\/j.gastro.2026.07.034_bib2","article-title":"Cellular origins of regenerating liver and hepatocellular carcinoma","volume":"4","author":"Holczbauer","year":"2022","journal-title":"JHEP Rep"},{"key":"10.1053\/j.gastro.2026.07.034_bib3","doi-asserted-by":"crossref","first-page":"5151","DOI":"10.3390\/cancers13205151","article-title":"Hepatocyte polyploidy: driver or gatekeeper of chronic liver diseases","volume":"13","author":"Donne","year":"2021","journal-title":"Cancers (Basel)"},{"key":"10.1053\/j.gastro.2026.07.034_bib4","article-title":"Hepatocyte polyploidization and its association with pathophysiological processes","volume":"8","author":"Wang","year":"2017","journal-title":"Cell Death Dis"},{"key":"10.1053\/j.gastro.2026.07.034_bib5","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.cell.2018.07.042","article-title":"Chromosome segregation fidelity in epithelia requires tissue architecture","volume":"175","author":"Knouse","year":"2018","journal-title":"Cell"},{"key":"10.1053\/j.gastro.2026.07.034_bib6","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1038\/nature08136","article-title":"A mechanism linking extra centrosomes to chromosomal instability","volume":"460","author":"Ganem","year":"2009","journal-title":"Nature"},{"key":"10.1053\/j.gastro.2026.07.034_bib7","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.ceb.2016.09.003","article-title":"Microtubule-organizing centers: from the centrosome to non-centrosomal sites","volume":"44","author":"Sanchez","year":"2017","journal-title":"Curr Opin Cell Biol"},{"key":"10.1053\/j.gastro.2026.07.034_bib8","doi-asserted-by":"crossref","first-page":"3281","DOI":"10.1242\/jcs.094797","article-title":"Never tear us apart\u2014the importance of centrosome clustering","volume":"125","author":"Marthiens","year":"2012","journal-title":"J Cell Sci"},{"key":"10.1053\/j.gastro.2026.07.034_bib9","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1083\/jcb.201704102","article-title":"Loss of E-cadherin provides tolerance to centrosome amplification in epithelial cancer cells","volume":"217","author":"Rhys","year":"2018","journal-title":"J Cell Biol"},{"key":"10.1053\/j.gastro.2026.07.034_bib10","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/nature09414","article-title":"The ploidy conveyor of mature hepatocytes as a source of genetic variation","volume":"467","author":"Duncan","year":"2010","journal-title":"Nature"},{"key":"10.1053\/j.gastro.2026.07.034_bib11","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1016\/j.cell.2008.05.039","article-title":"Centrosome amplification can initiate tumorigenesis in flies","volume":"133","author":"Basto","year":"2008","journal-title":"Cell"},{"key":"10.1053\/j.gastro.2026.07.034_bib12","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1038\/ng0895-453","article-title":"Pharmacological correction of neonatal lethal hepatic dysfunction in a murine model of hereditary tyrosinaemia type I","volume":"10","author":"Grompe","year":"1995","journal-title":"Nat Genet"},{"key":"10.1053\/j.gastro.2026.07.034_bib13","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1002\/hep.29626","article-title":"Combinatorial genetics in liver repopulation and carcinogenesis with a in vivo CRISPR activation platform","volume":"68","author":"Wangensteen","year":"2018","journal-title":"Hepatology"},{"key":"10.1053\/j.gastro.2026.07.034_bib14","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1053\/j.gastro.2023.02.043","article-title":"A therapeutically targetable TAZ-TEAD2 pathway drives the growth of hepatocellular carcinoma via ANLN and KIF23","volume":"164","author":"Saito","year":"2023","journal-title":"Gastroenterology"},{"key":"10.1053\/j.gastro.2026.07.034_bib15","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1016\/j.cell.2018.11.012","article-title":"Inflammatory cytokine TNFalpha promotes the long-term expansion of primary hepatocytes in 3D culture","volume":"175","author":"Peng","year":"2018","journal-title":"Cell"},{"key":"10.1053\/j.gastro.2026.07.034_bib16","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/978-3-319-55780-9_20","article-title":"Fah knockout animals as models for therapeutic liver repopulation","volume":"959","author":"Grompe","year":"2017","journal-title":"Adv Exp Med Biol"},{"key":"10.1053\/j.gastro.2026.07.034_bib17","doi-asserted-by":"crossref","first-page":"2297","DOI":"10.1172\/JCI95120","article-title":"TRAP-seq identifies cystine\/glutamate antiporter as a driver of recovery from liver injury","volume":"128","author":"Wang","year":"2018","journal-title":"J Clin Invest"},{"key":"10.1053\/j.gastro.2026.07.034_bib18","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1002\/hep.29562","article-title":"Distinct role of nuclear receptor corepressor 1 regulated de novo fatty acids synthesis in liver regeneration and hepatocarcinogenesis in mice","volume":"67","author":"Ou-Yang","year":"2018","journal-title":"Hepatology"},{"key":"10.1053\/j.gastro.2026.07.034_bib19","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.cell.2017.06.010","article-title":"Defining a cancer dependency map","volume":"170","author":"Tsherniak","year":"2017","journal-title":"Cell"},{"key":"10.1053\/j.gastro.2026.07.034_bib20","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.cellsig.2016.10.004","article-title":"The NHR domains of neuralized and related proteins: beyond Notch signalling","volume":"29","author":"Liu","year":"2017","journal-title":"Cell Signal"},{"key":"10.1053\/j.gastro.2026.07.034_bib21","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/S1534-5807(01)00092-2","article-title":"Drosophila neuralized is a ubiquitin ligase that promotes the internalization and degradation of delta","volume":"1","author":"Lai","year":"2001","journal-title":"Dev Cell"},{"key":"10.1053\/j.gastro.2026.07.034_bib22","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1038\/nature19356","article-title":"High-throughput discovery of novel developmental phenotypes","volume":"537","author":"Dickinson","year":"2016","journal-title":"Nature"},{"key":"10.1053\/j.gastro.2026.07.034_bib23","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/bs.mcb.2016.04.006","article-title":"Incomplete cytokinesis\/binucleation in mammals: the powerful system of hepatocytes","volume":"137","author":"Fortier","year":"2017","journal-title":"Methods Cell Biol"},{"key":"10.1053\/j.gastro.2026.07.034_bib24","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1038\/ncb2585","article-title":"E2F8 is essential for polyploidization in mammalian cells","volume":"14","author":"Pandit","year":"2012","journal-title":"Nat Cell Biol"},{"key":"10.1053\/j.gastro.2026.07.034_bib25","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1126\/science.add9528","article-title":"Control of cell proliferation by memories of mitosis","volume":"383","author":"Meitinger","year":"2024","journal-title":"Science"},{"key":"10.1053\/j.gastro.2026.07.034_bib26","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1038\/nrc2248","article-title":"The impact of a negligent G2\/M checkpoint on genomic instability and cancer induction","volume":"7","author":"Lobrich","year":"2007","journal-title":"Nat Rev Cancer"},{"key":"10.1053\/j.gastro.2026.07.034_bib27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1091\/mbc.e06-08-0753","article-title":"The NHR1 domain of Neuralized binds Delta and mediates Delta trafficking and Notch signaling","volume":"18","author":"Commisso","year":"2007","journal-title":"Mol Biol Cell"},{"key":"10.1053\/j.gastro.2026.07.034_bib28","doi-asserted-by":"crossref","first-page":"3182","DOI":"10.1038\/s41596-020-0365-x","article-title":"Combined proximity labeling and affinity purification-mass spectrometry workflow for mapping and visualizing protein interaction networks","volume":"15","author":"Liu","year":"2020","journal-title":"Nat Protoc"},{"key":"10.1053\/j.gastro.2026.07.034_bib29","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.33864","article-title":"Control of endothelial cell polarity and sprouting angiogenesis by non-centrosomal microtubules","volume":"7","author":"Martin","year":"2018","journal-title":"Elife"},{"key":"10.1053\/j.gastro.2026.07.034_bib30","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1083\/jcb.131.3.693","article-title":"NuMA is required for the organization of microtubules into aster-like mitotic arrays","volume":"131","author":"Gaglio","year":"1995","journal-title":"J Cell Biol"},{"key":"10.1053\/j.gastro.2026.07.034_bib31","doi-asserted-by":"crossref","DOI":"10.15252\/embj.2019102378","article-title":"NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells","volume":"39","author":"Chinen","year":"2020","journal-title":"EMBO J"},{"key":"10.1053\/j.gastro.2026.07.034_bib32","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.cub.2022.12.017","article-title":"NuMA deficiency causes micronuclei via checkpoint-insensitive k-fiber minus-end detachment from mitotic spindle poles","volume":"33","author":"van Toorn","year":"2023","journal-title":"Curr Biol"},{"key":"10.1053\/j.gastro.2026.07.034_bib33","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/j.cell.2007.06.025","article-title":"Self-organization of MTOCs replaces centrosome function during acentrosomal spindle assembly in live mouse oocytes","volume":"130","author":"Schuh","year":"2007","journal-title":"Cell"},{"key":"10.1053\/j.gastro.2026.07.034_bib34","doi-asserted-by":"crossref","first-page":"7217","DOI":"10.1038\/ncomms8217","article-title":"A three-step MTOC fragmentation mechanism facilitates bipolar spindle assembly in mouse oocytes","volume":"6","author":"Clift","year":"2015","journal-title":"Nat Commun"},{"key":"10.1053\/j.gastro.2026.07.034_bib35","doi-asserted-by":"crossref","DOI":"10.1126\/science.ado1022","article-title":"Mechanisms of minor pole-mediated spindle bipolarization in human oocytes","volume":"385","author":"Wu","year":"2024","journal-title":"Science"},{"key":"10.1053\/j.gastro.2026.07.034_bib36","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1038\/s41586-024-07376-2","article-title":"Multimodal decoding of human liver regeneration","volume":"630","author":"Matchett","year":"2024","journal-title":"Nature"},{"key":"10.1053\/j.gastro.2026.07.034_bib37","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.cell.2013.03.026","article-title":"A direct in vivo RNAi screen identifies MKK4 as a key regulator of liver regeneration","volume":"153","author":"Wuestefeld","year":"2013","journal-title":"Cell"},{"key":"10.1053\/j.gastro.2026.07.034_bib38","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1083\/jcb.120.4.947","article-title":"NuMA is required for the proper completion of mitosis","volume":"120","author":"Compton","year":"1993","journal-title":"J Cell Biol"},{"key":"10.1053\/j.gastro.2026.07.034_bib39","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1101\/gad.349727.122","article-title":"Centriole signaling restricts hepatocyte ploidy to maintain liver integrity","volume":"36","author":"Sladky","year":"2022","journal-title":"Genes Dev"},{"key":"10.1053\/j.gastro.2026.07.034_bib40","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.devcel.2019.12.016","article-title":"E2F-family members engage the PIDDosome to limit hepatocyte ploidy in liver development and regeneration","volume":"52","author":"Sladky","year":"2020","journal-title":"Dev Cell"},{"key":"10.1053\/j.gastro.2026.07.034_bib41","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.stem.2019.11.014","article-title":"In vivo lineage tracing of polyploid hepatocytes reveals extensive proliferation during liver regeneration","volume":"26","author":"Matsumoto","year":"2020","journal-title":"Cell Stem Cell"},{"key":"10.1053\/j.gastro.2026.07.034_bib42","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1038\/s41467-021-20916-y","article-title":"Proliferative polyploid cells give rise to tumors via ploidy reduction","volume":"12","author":"Matsumoto","year":"2021","journal-title":"Nat Commun"},{"key":"10.1053\/j.gastro.2026.07.034_bib43","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1747-1028-8-8","article-title":"To divide or not to divide: revisiting liver regeneration","volume":"8","author":"Miyaoka","year":"2013","journal-title":"Cell Div"},{"key":"10.1053\/j.gastro.2026.07.034_bib44","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1016\/j.cub.2012.05.016","article-title":"Hypertrophy and unconventional cell division of hepatocytes underlie liver regeneration","volume":"22","author":"Miyaoka","year":"2012","journal-title":"Curr Biol"}],"container-title":["Gastroenterology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0016508526071568?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0016508526071568?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:00:33Z","timestamp":1788235233000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0016508526071568"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":44,"alternative-id":["S0016508526071568"],"URL":"https:\/\/doi.org\/10.1053\/j.gastro.2026.07.034","relation":{},"ISSN":["0016-5085"],"issn-type":[{"value":"0016-5085","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Liver Regeneration CRISPR Screen Identifies E3 Ligase NEURL1B Regulates Hepatocyte Mitosis by Destabilizing Microtubule-Organizing Centers","name":"articletitle","label":"Article Title"},{"value":"Gastroenterology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1053\/j.gastro.2026.07.034","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 American Gastroenterological Association Institute. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}]}}