{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T14:16:16Z","timestamp":1783347376230,"version":"3.54.6"},"reference-count":43,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":5417,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>The anti\u2013mitochondrial antibody response in primary biliary cirrhosis (PBC) is primarily directed at E2 components of PDC, OGDC, and BCOADC, and E3BP. Previous work has shown that the immunodominant autoreactive T\u2013 cell epitope is the PDC\u2013E2 163\u2013176 peptide, restricted by HLA DR53. To address molecular mimicry and cross\u2013recognition among mitochondrial autoantigens, we analyzed reactivity, including agonism and antagonism assays, to a series of single amino acid-substituted peptides using cloned T\u2013cell lines in PBC and controls. Interestingly, fine specificities were unique for every single T\u2013cell clone, but the clones could be categorized into two distinct groups based on recognition motifs of the T\u2013cell receptor (TCR) ligand: group A <jats:sup>170<\/jats:sup>ExDK<jats:sup>173<\/jats:sup> and group B<jats:sup>168<\/jats:sup>EIExD<jats:sup>172<\/jats:sup>. <jats:sup>170<\/jats:sup>E is the most critical TCR contact residue for both groups of cloned T\u2013cell lines, whereas <jats:sup>173<\/jats:sup>K and <jats:sup>168<\/jats:sup>E are the critical TCR contact residues for group A and group B cloned T\u2013cell lines, respectively. More importantly, some group A-cloned T\u2013cell lines cross\u2013reacted to human E3BP 34\u201347, human OGDC\u2013E2 100\u2013113, and several peptides derived from various microbial proteins carrying an ExDK motif, whereas group B-cloned T\u2013cell lines reacted only to E3BP 34\u201347 carrying an EIExD motif. Furthermore, an RGxG motif was exclusively found in the complementarity\u2013determining region (CDR3) of the TCR V\u03b2 in the group B-cloned T\u2013cell lines, while G, S, and\/or R were frequently found in the CDR3 of the TCR V\u03b2 in the group A-cloned T\u2013cell lines. These data provide a framework for understanding molecular mimicry among mitochondrial antigens.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1053\/jhep.2000.18714","type":"journal-article","created":{"date-parts":[[2003,6,21]],"date-time":"2003-06-21T14:16:51Z","timestamp":1056205011000},"page":"901-909","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":62,"title":["Fine Specificity of T Cells Reactive to Human Pdc\u2013E2 163\u2013176 Peptide, the Immunodominant Autoantigen in Primary Biliary Cirrhosis: Implications for Molecular Mimicry and Cross\u2013Recognition Among Mitochondrial Autoantigens"],"prefix":"10.1097","volume":"32","author":[{"given":"Hirohisa","family":"Shigematsu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Shimoda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoru","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sho","family":"Matsushita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuharu","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihiro","family":"Sakamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasunori","family":"Ichiki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Niho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric 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