{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:13:58Z","timestamp":1787004838953,"version":"build-2736575974"},"reference-count":45,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":5509,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,8]]},"abstract":"<jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Proteome analysis was performed on cellular and secreted proteins of normal (quiescent) and activated rat hepatic stellate cells. The stellate cells were activated either\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      by cultivating quiescent stellate cells for 9 days or\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      by injecting rats with carbon tetrachloride for 8 weeks. A total of 43 proteins\/polypeptides were identified, which altered their expression levels when the cells were activated\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      and\/or\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      . Twenty-seven of them showed similar changes\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      , including up-regulated proteins such as calcyclin, calgizzarin, and galectin-1 as well as down-regulated proteins such as liver carboxylesterase 10 and serine protease inhibitor 3. Sixteen of them showed different expression levels between\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      activated stellate cells. These results were reproducibly obtained in 3 independent experiments. The up-regulation of calcyclin, calgizzarin, and galectin-1, as well as the down-regulation of liver carboxylesterase 10 were directly confirmed in fibrotic liver tissues. Northern blots confirmed up-regulation of the messenger RNAs (mRNAs) of calcyclin, calgizzarin, and galectin-1 in activated stellate cells, indicating that these changes were controlled at the mRNA level. In addition a list compiling over 150 stellate cell proteins is presented. The data presented here thus provide a significant new protein-level insight into the activation of hepatic stellate cells, a key event in liver fibrogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1053\/jhep.2000.9322","type":"journal-article","created":{"date-parts":[[2004,3,19]],"date-time":"2004-03-19T17:03:01Z","timestamp":1079715781000},"page":"268-277","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":174,"title":["Proteome analysis of rat hepatic stellate cells"],"prefix":"10.1097","volume":"32","author":[{"given":"Dan Bach","family":"Kristensen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norifumi","family":"Kawada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunihiko","family":"Imamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka","family":"Miyamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chise","family":"Tateno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuichi","family":"Seki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuo","family":"Kuroki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsutoshi","family":"Yoshizato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"10.1053\/jhep.2000.9322-BIB1","first-page":"1069","volume":"12","author":"Kawada","year":"1997","journal-title":"Histol Histopathol"},{"key":"10.1053\/jhep.2000.9322-BIB2","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1007\/s004410051073","volume":"292","author":"Gressner","year":"1998","journal-title":"Cell Tissue Res"},{"key":"10.1053\/jhep.2000.9322-BIB3","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/S0168-8278(98)80269-9","volume":"29","author":"Olaso","year":"1998","journal-title":"J Hepatol"},{"key":"10.1053\/jhep.2000.9322-BIB4","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1126\/science.270.5235.369","volume":"270","author":"Kahn","year":"1995","journal-title":"Science"},{"key":"10.1053\/jhep.2000.9322-BIB5","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1002\/elps.1150191103","volume":"19","author":"Anderson","year":"1998","journal-title":"Electrophoresis"},{"key":"10.1053\/jhep.2000.9322-BIB6","doi-asserted-by":"crossref","first-page":"4007","DOI":"10.1016\/S0021-9258(19)41496-8","volume":"250","author":"O'Farrell","year":"1975","journal-title":"J Biol Chem"},{"key":"10.1053\/jhep.2000.9322-BIB7","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/S0958-1669(97)80151-6","volume":"8","author":"Roepstorff","year":"1997","journal-title":"Curr Opin Biotechnol"},{"key":"10.1053\/jhep.2000.9322-BIB8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/(SICI)1096-9888(199801)33:1<1::AID-JMS624>3.0.CO;2-9","volume":"33","author":"Yates","year":"1998","journal-title":"J Mass Spectrom"},{"key":"10.1053\/jhep.2000.9322-BIB9","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1023\/A:1026330015280","volume":"16","author":"Humphery Smith","year":"1997","journal-title":"J Protein Chem"},{"key":"10.1053\/jhep.2000.9322-BIB10","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1111\/j.1432-1033.1993.tb17824.x","volume":"213","author":"Kawada","year":"1993","journal-title":"Eur J Biochem"},{"key":"10.1053\/jhep.2000.9322-BIB11","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1002\/(SICI)1522-2683(20000101)21:2<430::AID-ELPS430>3.0.CO;2-0","volume":"21","author":"Kristensen","year":"2000","journal-title":"Electrophoresis"},{"key":"10.1053\/jhep.2000.9322-BIB12","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1002\/elps.11501501223","volume":"15","author":"Rabilloud","year":"1994","journal-title":"Electrophoresis"},{"key":"10.1053\/jhep.2000.9322-BIB13","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1002\/elps.1150180305","volume":"18","author":"Sanchez","year":"1997","journal-title":"Electrophoresis"},{"key":"10.1053\/jhep.2000.9322-BIB14","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/S0168-8278(96)80269-8","volume":"24","author":"Neubauer","year":"1996","journal-title":"J Hepatol"},{"key":"10.1053\/jhep.2000.9322-BIB15","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1002\/path.1711500307","volume":"150","author":"Collier","year":"1986","journal-title":"J Pathol"},{"key":"10.1053\/jhep.2000.9322-BIB16","first-page":"1","volume-title":"Cell separation: methods and selected applications.","volume":"4","author":"Pertoft","year":"1982","unstructured":". 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