{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:57:15Z","timestamp":1791176235158,"version":"4.1.0"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":5356,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Hepatitis C virus (HCV)\u2013specific CD8<jats:sup>+<\/jats:sup> T cells in peripheral blood mononuclear cells (PBMCs) from patients infected with HCV were quantitatively analyzed by flow cytometry using an HLA\u2013B*3501\u2013HCV epitope tetrameric complex. In chronic hepatitis C, tetramer<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T cells were detected at frequencies ranging from 0.05% to 0.12% of total CD8<jats:sup>+<\/jats:sup> T cells. The number of tetramer<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T cells in acute phase PBMCs from patients with acute hepatitis C was about 3 to 5 times higher than in recovery phase PBMCs from the same patients and in PBMCs from patients with chronic hepatitis C. Expanding tetramer<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T cells in PBMCs from patients with acute hepatitis C express a CD28<jats:sup>+<\/jats:sup>CD45RA<jats:sup>-<\/jats:sup> memory T\u2013cell phenotype. In contrast, tetramer<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T cells in PBMCs from patients with chronic hepatitis C did not predominantly express this phenotype. These tetramer<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T cells did not have perforin in their cytoplasma. The present study visually showed that a high number of circulating HCV\u2013specific CD8<jats:sup>+<\/jats:sup> T cells in acute phase PBMCs from patients with acute hepatitis C are mostly memory T cells.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1053\/jhep.2001.21164","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T03:46:10Z","timestamp":1030247170000},"page":"287-294","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":31,"title":["Visual Demonstration of Hepatitis C Virus\u2013Specific Memory Cd8+ T\u2013Cell Expansion in Patients With Acute Hepatitis C"],"prefix":"10.1097","volume":"33","author":[{"given":"Yuji","family":"Sobao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Tomiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saburo","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisahiko","family":"Sekihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuaki","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masafumi","family":"Takiguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1053%2Fjhep.2001.21164","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.lww.com\/01515467-200101000-00036","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T11:30:25Z","timestamp":1733052625000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-200101000-00036"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001]]}},"URL":"https:\/\/doi.org\/10.1053\/jhep.2001.21164","relation":{},"ISSN":["0270-9139"],"issn-type":[{"value":"0270-9139","type":"print"}],"subject":[],"published":{"date-parts":[[2001,1]]}}}