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The effect of nonsteroidal anti-inflammatory drugs (NSAIDs) on the inflammatory onset was analyzed in these cells since in addition to the inhibition of cyclooxygenase activity, these drugs interfere with other signaling pathways related with the inflammatory response. Inhibition of nuclear factor \u03baB (NF-\u03baB) activation by aspirin and salicylate has been described in many cells. However, incubation of hepatic cells with salicylate, aspirin, indomethacin, ibuprofen, or 5,5-dimethyl-3(3-fluorophenyl)-4-(4-methylsulfonyl)phenyl-2(5H)-furanone (DFU), a fluorinated derivative of rofecoxib, failed to impair I\u03baB kinase activity, the processing of NF-\u03baB, and the expression of NF-\u03baB-dependent genes, such as NOS-2. Moreover, selective COX-2 inhibitors did not promote apoptosis in hepatocytes under inflammatory conditions, suggesting that prostaglandins are not required to maintain cell viability. In conclusion, these data indicate that hepatocytes are not sensitive to NF-\u03baB inhibition by NSAIDs and that these drugs, especially the COX-2 selective inhibitors, do not alter cell viability.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1053\/jhep.2002.31163","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T03:46:10Z","timestamp":1030247170000},"page":"341-348","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":19,"title":["Absence of nuclear factor \u03baB inhibition by NSAIDs in hepatocytes"],"prefix":"10.1097","volume":"35","author":[{"given":"Nuria A.","family":"Callejas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Casado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisardo","family":"Bosc\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paloma","family":"Mart\u00edn-Sanz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1053\/jhep.2002.31163-BIB1|cit1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/0005-2760(91)90032-D","article-title":"Prostaglandin endoperoxide synthase: regulation of enzyme expression","volume":"1083","author":"DeWitt","year":"1991","journal-title":"Biochim Biophys Acta"},{"key":"10.1053\/jhep.2002.31163-BIB2|cit2","doi-asserted-by":"crossref","first-page":"33157","DOI":"10.1074\/jbc.271.52.33157","article-title":"Prostaglandin endoperoxide H synthases (cyclooxygenases)-1 and -2","volume":"271","author":"Smith","year":"1996","journal-title":"J Biol Chem"},{"key":"10.1053\/jhep.2002.31163-BIB3|cit3","doi-asserted-by":"crossref","first-page":"7908","DOI":"10.1038\/sj.onc.1203286","article-title":"The role of cyclooxygenases in inflammation, cancer, and development","volume":"18","author":"Williams","year":"1999","journal-title":"Oncogene"},{"key":"10.1053\/jhep.2002.31163-BIB4|cit4","doi-asserted-by":"crossref","first-page":"25643","DOI":"10.1016\/S0021-9258(19)74438-X","article-title":"Differential regulation of inducible and constitutive prostaglandin endoperoxide synthase in osteoblastic MC3T3-E1 cells","volume":"268","author":"Pilbeam","year":"1993","journal-title":"J Biol Chem"},{"key":"10.1053\/jhep.2002.31163-BIB5|cit5","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1172\/JCI117842","article-title":"Involvement of reactive oxygen intermediates in cyclooxygenase-2 expression induced by interleukin-1, tumor necrosis factor-\u03b1, and lipopolysaccharide","volume":"95","author":"Feng","year":"1995","journal-title":"J Clin Invest"},{"key":"10.1053\/jhep.2002.31163-BIB6|cit6","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1111\/j.1749-6632.1999.tb08723.x","article-title":"Cyclooxygenase-deficient mice. 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