{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T03:47:39Z","timestamp":1790135259474,"version":"4.0.1"},"reference-count":41,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":4595,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Liver fibrosis is characterized by a dramatic increase in the expression of type I collagen. Several deoxyribonuclease (DNase) I-hypersensitive sites (HS) have been located in the distal 5\u2032-flanking region of the \u03b11(I) collagen gene that are specific to collagen-producing cells. To assess the role of the DNase I-HS in regulating \u03b11(I) collagen gene expression in hepatic stellate cells (HSCs), 3 transgenic mouse lines expressing collagen-\u03b11(I) reporter genes were used (Krempen et al. Gene Expr 1999;8:151-163). The pCol9GFP transgene contains the collagen gene promoter (\u22123122 to +111) linked to the green fluorescent protein (GFP) reporter gene. The pCol9GFP-HS4,5 transgene contains HS4,5 and pColGFP-HS8,9 contains HS8,9 positioned upstream of the collagen promoter in pCol9GFP. HSCs isolated from transgenic mice containing pCol9GFPHS4,5 and pColGFP-HS8,9 showed earlier and higher GFP expression patterns than HSCs isolated from pCol9GFP mice. HSCs from pCol9GFP-HS4,5 showed the highest levels of GFP expression and culture-induced expression correlated with induction of the endogenous \u03b11(I) collagen gene. After CCl<jats:sub>4<\/jats:sub> administration, pCol9GFP-HS4,5 mice showed increased GFP expression compared with pCol9GFP mice in both whole liver extracts and isolated HSCs. Several sites for DNA-protein interactions in both HS4 and HS5 were identified that included a binding site for activator protein 1. In conclusion, DNase I-HS4,5 enhance expression of the \u03b11(I) collagen gene promoter in HSCs both <jats:italic toggle=\"yes\">in vitro<\/jats:italic> and <jats:italic toggle=\"yes\">in vivo<\/jats:italic> after a fibrogenic stimulus. The collagen-GFP transgenic mice provide a convenient and reliable model system to investigate the molecular mechanisms controlling increased collagen expression during fibrosis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1053\/jhep.2003.50067","type":"journal-article","created":{"date-parts":[[2003,2,5]],"date-time":"2003-02-05T02:43:33Z","timestamp":1044413013000},"page":"267-276","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":203,"title":["DNase I-hypersensitive sites enhance \u03b11(I) collagen gene expression in hepatic stellate cells"],"prefix":"10.1097","volume":"37","author":[{"given":"Yutaka","family":"Yata","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Scanga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Gillan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimon","family":"Reif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Breindl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Brenner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Rippe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"10.1053\/jhep.2003.50067-BIB1","first-page":"101","volume":"14","author":"Friedman","year":"1996","journal-title":"Prog Liver Dis"},{"key":"10.1053\/jhep.2003.50067-BIB2","doi-asserted-by":"crossref","first-page":"g7","DOI":"10.1152\/ajpgi.2000.279.1.G7","volume":"279","author":"Eng","year":"2000","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"10.1053\/jhep.2003.50067-BIB3","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1146\/annurev.bi.59.070190.004201","volume":"59","author":"Vuoria","year":"1990","journal-title":"Annu Rev Biochem"},{"key":"10.1053\/jhep.2003.50067-BIB4","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1002\/ajmg.1320450203","volume":"45","author":"Slack","year":"1993","journal-title":"Am J Med Genet"},{"key":"10.1053\/jhep.2003.50067-BIB5","first-page":"755","volume":"124","author":"Brenner","year":"1994","journal-title":"J Lab Clin Med"},{"key":"10.1053\/jhep.2003.50067-BIB6","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0945-053X(96)90121-3","volume":"15","author":"Bornstein","year":"1996","journal-title":"Matrix Biol"},{"key":"10.1053\/jhep.2003.50067-BIB7","first-page":"241","volume":"22","author":"Rippe","year":"1995","journal-title":"Hepatology"},{"key":"10.1053\/jhep.2003.50067-BIB8","doi-asserted-by":"crossref","first-page":"5201","DOI":"10.1128\/MCB.17.9.5201","volume":"17","author":"Stefanovic","year":"1997","journal-title":"Mol Cell Biol"},{"key":"10.1053\/jhep.2003.50067-BIB9","doi-asserted-by":"crossref","first-page":"4334","DOI":"10.1128\/MCB.19.6.4334","volume":"19","author":"Stefanovic","year":"1999","journal-title":"Mol Cell Biol"},{"key":"10.1053\/jhep.2003.50067-BIB10","doi-asserted-by":"crossref","first-page":"4065","DOI":"10.1128\/MCB.11.8.4065","volume":"11","author":"Nehls","year":"1991","journal-title":"Mol Cell Biol"},{"key":"10.1053\/jhep.2003.50067-BIB11","doi-asserted-by":"crossref","first-page":"8869","DOI":"10.1073\/pnas.84.24.8869","volume":"84","year":"1987","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1053\/jhep.2003.50067-BIB12","doi-asserted-by":"crossref","first-page":"2224","DOI":"10.1128\/MCB.9.5.2224","volume":"9","author":"Rippe","year":"1989","journal-title":"Mol Cell Biol"},{"key":"10.1053\/jhep.2003.50067-BIB13","doi-asserted-by":"crossref","first-page":"9934","DOI":"10.1016\/S0021-9258(19)38761-7","volume":"265","author":"Karsenty","year":"1990","journal-title":"J Biol Chem"},{"key":"10.1053\/jhep.2003.50067-BIB14","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1016\/S0021-9258(17)35290-0","volume":"266","author":"Olsen","year":"1991","journal-title":"J Biol Chem"},{"key":"10.1053\/jhep.2003.50067-BIB15","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1007\/BF00299391","volume":"118","author":"Katai","year":"1992","journal-title":"Mol Cell Biochem"},{"key":"10.1053\/jhep.2003.50067-BIB16","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1042\/bj2860179","volume":"286","author":"Simkevich","year":"1992","journal-title":"Biochem J"},{"key":"10.1053\/jhep.2003.50067-BIB17","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1002\/jbmr.5650090218","volume":"9","author":"Bogdanovic","year":"1994","journal-title":"J Bone Miner Res"},{"key":"10.1053\/jhep.2003.50067-BIB18","doi-asserted-by":"crossref","first-page":"14828","DOI":"10.1016\/S0021-9258(17)36699-1","volume":"269","author":"Inagaki","year":"1994","journal-title":"J Biol Chem"},{"key":"10.1053\/jhep.2003.50067-BIB19","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1083\/jcb.125.3.695","volume":"125","author":"Liska","year":"1994","journal-title":"J Cell Biol"},{"key":"10.1053\/jhep.2003.50067-BIB20","doi-asserted-by":"crossref","first-page":"5950","DOI":"10.1128\/MCB.14.9.5950","volume":"14","author":"Rhodes","year":"1994","journal-title":"Mol Cell Biol"},{"key":"10.1053\/jhep.2003.50067-BIB21","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1074\/jbc.272.3.1753","volume":"272","author":"Rippe","year":"1997","journal-title":"J Biol Chem"},{"key":"10.1053\/jhep.2003.50067-BIB22","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1089\/104454999314890","volume":"18","author":"Rippe","year":"1999","journal-title":"DNA Cell Biol"},{"key":"10.1053\/jhep.2003.50067-BIB23","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1089\/10445490050043317","volume":"19","author":"Inmaculada","year":"2000","journal-title":"DNA Cell Biol"},{"key":"10.1053\/jhep.2003.50067-BIB24","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0378-1119(97)00293-X","volume":"198","author":"Salimi-Tari","year":"1997","journal-title":"Gene"},{"key":"10.1053\/jhep.2003.50067-BIB25","first-page":"151","volume":"8","author":"Krempen","year":"1999","journal-title":"Gene Expression"},{"key":"10.1053\/jhep.2003.50067-BIB26","first-page":"459","volume":"160","author":"Rippe,","year":"2001","journal-title":"Methods Mol Biol"},{"key":"10.1053\/jhep.2003.50067-BIB27","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1093\/nar\/26.1.362","volume":"26","author":"Heinemeyer","year":"1998","journal-title":"Nucleic Acids Res"},{"key":"10.1053\/jhep.2003.50067-BIB28","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1016\/S0016-5085(99)70407-5","volume":"117","author":"Knittel","year":"1999","journal-title":"Gastroenterology"},{"key":"10.1053\/jhep.2003.50067-BIB29","first-page":"g589","volume":"264","author":"Brenner","year":"1993","journal-title":"Am J Physiol"},{"key":"10.1053\/jhep.2003.50067-BIB30","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.1128\/MCB.11.4.2066","volume":"11","author":"Slack","year":"1991","journal-title":"Mol Cell Biol"},{"key":"10.1053\/jhep.2003.50067-BIB31","first-page":"287","volume":"17","author":"Brenner","year":"1993","journal-title":"Hepatology"},{"key":"10.1053\/jhep.2003.50067-BIB32","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1002\/jbmr.5650101004","volume":"10","author":"Bedalov","year":"1995","journal-title":"J Bone Miner Res"},{"key":"10.1053\/jhep.2003.50067-BIB33","doi-asserted-by":"crossref","first-page":"2269","DOI":"10.1172\/JCI118282","volume":"96","author":"Houglum","year":"1995","journal-title":"J Clin Invest"},{"key":"10.1053\/jhep.2003.50067-BIB34","doi-asserted-by":"crossref","first-page":"9622","DOI":"10.1074\/jbc.270.16.9622","volume":"270","author":"Sokolov","year":"1995","journal-title":"J Biol Chem"},{"key":"10.1053\/jhep.2003.50067-BIB35","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1073\/pnas.93.3.1027","volume":"93","author":"Rossert","year":"1996","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1053\/jhep.2003.50067-BIB36","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1046\/j.1432-1327.2000.01135.x","volume":"267","author":"Nakamachi","year":"2000","journal-title":"Eur J Biochem"},{"key":"10.1053\/jhep.2003.50067-BIB37","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1002\/bies.950160306","volume":"16","author":"Wallrath","year":"1994","journal-title":"Bioessays"},{"key":"10.1053\/jhep.2003.50067-BIB38","first-page":"279","volume":"162A","author":"Boulikas","year":"1995","journal-title":"Int Rev Cytol"},{"key":"10.1053\/jhep.2003.50067-BIB39","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/S0378-1119(99)00340-6","volume":"238","author":"Bonifer","year":"1999","journal-title":"Gene"},{"key":"10.1053\/jhep.2003.50067-BIB40","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1083\/jcb.134.5.1333","volume":"134","author":"Bou-Gharios","year":"1996","journal-title":"J Cell Biol"},{"key":"10.1053\/jhep.2003.50067-BIB41","doi-asserted-by":"crossref","first-page":"21754","DOI":"10.1074\/jbc.M101397200","volume":"276","author":"Antoniv","year":"2001","journal-title":"J Biol Chem"}],"container-title":["Hepatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.lww.com\/01515467-200302000-00008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T11:29:40Z","timestamp":1733052580000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.lww.com\/01515467-200302000-00008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,2]]},"references-count":41,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003]]}},"URL":"https:\/\/doi.org\/10.1053\/jhep.2003.50067","relation":{},"ISSN":["0270-9139"],"issn-type":[{"value":"0270-9139","type":"print"}],"subject":[],"published":{"date-parts":[[2003,2]]}}}