{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T02:17:28Z","timestamp":1777515448544,"version":"3.51.4"},"reference-count":47,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":4475,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:sec>\n            <jats:title\/>\n            <jats:p>Thrombocytopenia is a common manifestation in patients with liver cirrhosis (LC), but its underlying mechanism remains controversial. This study examined the role of anti\u2013platelet autoimmunity in cirrhotic thrombocytopenia by determining the autoantibody response to GPIIb\u2013IIIa, a major platelet surface autoantigen recognized by anti\u2013platelet antibodies in patients with idiopathic thrombocytopenic purpura (ITP). Circulating B cells producing anti\u2013GPIIb\u2013IIIa antibodies as well as platelet\u2013associated and plasma anti\u2013GPIIb\u2013IIIa antibodies were examined in 72 patients with LC, 62 patients with ITP, and 52 healthy controls. <jats:italic toggle=\"yes\">In vitro<\/jats:italic> anti\u2013GPIIb\u2013IIIa antibody production was induced in cultures of peripheral blood mononuclear cells (PBMCs) by stimulation with GPIIb\u2013IIIa. The frequency of anti\u2013GPIIb\u2013IIIa antibody\u2013producing B cells in patients with LC was significantly greater than in healthy controls (10.9 \u00b1 6.2 vs. 0.4 \u00b1 0.3\/10<jats:sup>5<\/jats:sup> PBMCs; <jats:italic toggle=\"yes\">P<\/jats:italic> &lt; .0001) and was even higher than the frequency in patients with ITP (8.2 \u00b1 5.2; <jats:italic toggle=\"yes\">P<\/jats:italic> = .007). Anti\u2013GPIIb\u2013IIIa antibodies in the patients with LC and ITP were mainly present on the surfaces of circulating platelets rather than in the plasma in an unbound form. Furthermore, PBMCs from patients with LC and ITP produced anti\u2013GPIIb\u2013IIIa antibodies on antigenic stimulation with GPIIb\u2013IIIa <jats:italic toggle=\"yes\">in vitro<\/jats:italic>, and the specific antibodies produced had the capacity to bind normal platelet surfaces. In conclusion, the similar profile of the anti\u2013GPIIb\u2013IIIa autoantibody response in patients with LC and ITP suggests that autoantibody\u2013mediated platelet destruction may contribute at least in part to cirrhotic thrombocytopenia.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1053\/jhep.2003.50209","type":"journal-article","created":{"date-parts":[[2003,5,28]],"date-time":"2003-05-28T22:17:49Z","timestamp":1054160269000},"page":"1267-1276","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":89,"title":["A Role of Autoantibody\u2013Mediated Platelet Destruction in Thrombocytopenia in Patients With Cirrhosis"],"prefix":"10.1097","volume":"37","author":[{"given":"Mikio","family":"Kajihara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinzo","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuka","family":"Okazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Kawakami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromasa","family":"Ishii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuo","family":"Ikeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masataka","family":"Kuwana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"10.1053\/jhep.2003.50209-BIB1","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1172\/JCI105380","volume":"45","author":"Aster","year":"1966","journal-title":"J 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