{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T10:25:45Z","timestamp":1761647145826},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,4,29]],"date-time":"2004-04-29T00:00:00Z","timestamp":1083196800000},"content-version":"vor","delay-in-days":1155,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Surgery"],"published-print":{"date-parts":[[2001,3]]},"abstract":"<jats:p><jats:bold>Objective\u2014<\/jats:bold>To investigate the potential of doxycycline to reduce stromelysin and inducible nitric oxide synthase (iNOS) activity in dogs with osteoarthritis (OA) secondary to spontaneous cranial cruciate ligament (CCL) rupture.<\/jats:p><jats:p><jats:bold>Study Design\u2014<\/jats:bold>Prospective, clinical study.<\/jats:p><jats:p><jats:bold>Animals\u2014<\/jats:bold>Eighty\u2010one dogs with OA secondary to CCL rupture and 54 normal dogs.<\/jats:p><jats:p><jats:bold>Methods\u2014<\/jats:bold>Dogs with OA secondary to CCL rupture were divided into 2 groups before surgery. The Doxy\u2010CCl group received 3 to 4 mg\/kg doxycycline orally every 24 hours for 7 to 10 days (<jats:styled-content>n = 35<\/jats:styled-content>). The CCL group received no treatment (<jats:styled-content>n = 46<\/jats:styled-content>). Synovial fluid, articular cartilage, synovial membrane, and CCL samples were collected during surgery (Doxy\u2010CCL group and CCL group) or immediately after euthanasia from healthy dogs (control group). Synovial fluid samples were examined cytologically. Total nitric oxide (NO<jats:sub>t<\/jats:sub>) concentrations were measured in the supernatant of explant cultures of all tissue samples, and stromelysin activity was measured in the supernatant of explant cultures of cartilage.<\/jats:p><jats:p><jats:bold>Results\u2014<\/jats:bold>NO<jats:sub>t<\/jats:sub>concentrations measured in cartilage were significantly lower in the Doxy\u2010CCL group than in the CCL group, but were not different from those measured in the control group. Doxycycline treatment did not have a significant effect on cartilage stromelysin levels.<\/jats:p><jats:p><jats:bold>Conclusion\u2014<\/jats:bold>The findings in this study indicate that doxycycline inhibits NO production in cartilage in dogs with CCL rupture.<\/jats:p><jats:p><jats:bold>Clinical Relevance\u2014<\/jats:bold>Doxycycline may have a role in the treatment of canine OA by inhibiting NO production.<\/jats:p>","DOI":"10.1053\/jvet.2001.21399","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T03:46:10Z","timestamp":1030247170000},"page":"132-139","source":"Crossref","is-referenced-by-count":16,"title":["The Effects of Doxycycline on Nitric Oxide and Stromelysin Production in Dogs With Cranial Cruciate Ligament Rupture"],"prefix":"10.1111","volume":"30","author":[{"given":"Silvia","family":"Jauernig","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariane","family":"Schweighauser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Reist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitte","family":"Von Rechenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Schawalder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Spreng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,4,29]]},"reference":[{"key":"e_1_2_14_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1063-4584(96)80009-4"},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199807)41:7<1275::AID-ART19>3.0.CO;2-T"},{"key":"e_1_2_14_4_2","first-page":"109","article-title":"Synthesis of metalloproteinases and interleukin\u20106 (IL\u20106) in human osteoarthritic synovial membrane is an IL\u20101 mediated process","volume":"43","author":"Pelletier JP","year":"1995","journal-title":"J Rheumatol"},{"key":"e_1_2_14_5_2","first-page":"1969","volume-title":"Arthritis and Allied Conditions: A Textbook of Rheumatology","author":"Pelletier JP","year":"1997"},{"key":"e_1_2_14_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.1003"},{"key":"e_1_2_14_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.24.14014"},{"key":"e_1_2_14_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780370712"},{"key":"e_1_2_14_9_2","first-page":"353","article-title":"Increased expression of the inducible nitric oxide synthase in human osteoarthritic chondrocytes","volume":"20","author":"Cesare PE","year":"1995","journal-title":"Trans Orthop Res Soc"},{"key":"e_1_2_14_10_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119280"},{"key":"e_1_2_14_11_2","first-page":"275","article-title":"Nitric oxide and its role on orthopaedic disease","volume":"312","author":"Evans CH","year":"1995","journal-title":"Clin Orthop"},{"key":"e_1_2_14_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.55.1.7"},{"key":"e_1_2_14_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.2.749"},{"key":"e_1_2_14_14_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2000.61.530"},{"key":"e_1_2_14_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.1426"},{"key":"e_1_2_14_16_2","doi-asserted-by":"publisher","DOI":"10.1053\/joca.1998.0220"},{"key":"e_1_2_14_17_2","first-page":"693","article-title":"Elevated metallopreoteinase and tissue inhibitor of metalloproteinase mRNA in human osteoarthritic synovia","volume":"20","author":"Zafarullah M","year":"1993","journal-title":"J Rheumatol"},{"key":"e_1_2_14_18_2","first-page":"25","article-title":"Metalloproteinases and tissue damage","volume":"30","author":"Murphy G","year":"1991","journal-title":"Br J Rheum"},{"key":"e_1_2_14_19_2","doi-asserted-by":"crossref","first-page":"8683","DOI":"10.1016\/S0021-9258(18)31499-6","article-title":"Catabolism of aggrecan in cartilage explants. 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