{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T15:24:59Z","timestamp":1780068299240,"version":"3.54.0"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"12","license":[{"start":{"date-parts":[[2018,12,10]],"date-time":"2018-12-10T00:00:00Z","timestamp":1544400000000},"content-version":"vor","delay-in-days":9,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Endosc Int Open"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Background\u2002Despite advances in the diagnosis of pancreatic ductal adenocarcinoma (PDA), histological evaluation of small and poorly defined masses in the pancreas is uncomfortable and unsafe.<\/jats:p><jats:p>\n          Methods\u2002We herein report a case of early stage PDA, in which multiple KRAS mutations were detected in the pancreatic juice preoperatively. A small hypoechoic area adjacent to the portal vein was detected through endoscopic ultrasound in the pancreatic body. KRAS mutations were evaluated using plasma, and the pancreatic juice by digital PCR.<\/jats:p><jats:p>\n          Results\u2002Pancreatic duct biopsy and pancreatic juice cytology were performed with no evidence of malignancy; however, KRAS mutations, KRAS G12V and G12D, were detected in the pancreatic juice. Histological assessment of the resected specimen demonstrated a solid tumor with desmoplastic reaction accompanied by carcinoma in situ in the main pancreatic duct where KRAS G12V mutation was identified. More detailed analysis demonstrated KRAS G12D mutation in the cluster of low grade pancreatic intraepithelial neoplasia, implying that the lesion developed independently.<\/jats:p><jats:p>\n          Conclusions\u2002Our study indicates the potential of \u201cendoscopic liquid biopsy\u201d to capture the driver gene for PDA diagnosis.<\/jats:p>","DOI":"10.1055\/a-0721-1747","type":"journal-article","created":{"date-parts":[[2018,12,11]],"date-time":"2018-12-11T10:45:36Z","timestamp":1544525136000},"page":"E1454-E1461","source":"Crossref","is-referenced-by-count":16,"title":["Utility of \u201cliquid biopsy\u201d using pancreatic juice for early detection of pancreatic cancer"],"prefix":"10.1055","volume":"06","author":[{"given":"Tetsuhiro","family":"Okada","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Research, Sapporo Higashi Tokushukai Hospital, Sapporo, Hokkaido 065-0033, Japan"},{"name":"Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirotoshi","family":"Iwano","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Endoscopic Unit, Shibetsu City Hospital, Shibetsu, Hokkaido 095-0048, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Ono","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Research, Sapporo Higashi Tokushukai Hospital, Sapporo, Hokkaido 065-0033, Japan"},{"name":"Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidenori","family":"Karasaki","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Research, Sapporo Higashi Tokushukai Hospital, Sapporo, Hokkaido 065-0033, Japan"},{"name":"Department of Surgery, Sapporo Higashi Tokushukai Hospital, Sapporo, Hokkaido 065-0033, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Endoscopic Unit, Shibetsu City Hospital, Shibetsu, Hokkaido 095-0048, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masataka","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Endoscopic Unit, Shibetsu City Hospital, Shibetsu, Hokkaido 095-0048, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Omori","sequence":"additional","affiliation":[{"name":"Department of Histopathology, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980-8575, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiro","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidemasa","family":"Kawabata","sequence":"additional","affiliation":[{"name":"Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuma","family":"Goto","sequence":"additional","affiliation":[{"name":"Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junpei","family":"Sasajima","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Research, Sapporo Higashi Tokushukai Hospital, Sapporo, Hokkaido 065-0033, Japan"},{"name":"Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhei","family":"Takauji","sequence":"additional","affiliation":[{"name":"Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Nagashima","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Research, Sapporo Higashi Tokushukai Hospital, Sapporo, Hokkaido 065-0033, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Mizukami","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Research, Sapporo Higashi Tokushukai Hospital, Sapporo, Hokkaido 065-0033, Japan"},{"name":"Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshikatsu","family":"Okumura","sequence":"additional","affiliation":[{"name":"Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2018,12,10]]},"container-title":["Endoscopy International Open"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-0721-1747.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T20:01:56Z","timestamp":1557172916000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-0721-1747"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12]]},"references-count":0,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2018,11,21]]},"published-print":{"date-parts":[[2018,12]]}},"URL":"https:\/\/doi.org\/10.1055\/a-0721-1747","relation":{},"ISSN":["2364-3722","2196-9736"],"issn-type":[{"value":"2364-3722","type":"print"},{"value":"2196-9736","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12]]}}}