{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T10:55:31Z","timestamp":1772103331330,"version":"3.50.1"},"reference-count":31,"publisher":"Georg Thieme Verlag KG","issue":"05","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Bryophyllum pinnatum has been used since the 1970s to prevent premature\n                    labour, first in anthroposophic hospitals and, more recently, also in the main\n                    Swiss perinatal centres. However, it is not known which compounds in B.\n                        pinnatum leaves contribute to the tocolytic effect. Here we studied the\n                    effects of a flavonoid-enriched fraction, the corresponding flavonoid aglycon\n                    mixture, a bufadienolide-enriched fraction, and B.\u00a0pinnatum leaf press\n                    juice on human myometrial contractility in vitro. The strength (area\n                    under the curve and amplitude) and frequency of contractions were recorded using\n                    strips of human myometrium mounted in an organ bath system. Cell viability\n                    assays were performed with the human myometrium hTERT-C3 and PHM1\u200a\u2013\u200a41 cell\n                    lines. Repeated addition of the flavonoid-enriched fraction, flavonoid aglycon\n                    mixture, bufadienolide-enriched fraction, or B. pinnatum leaf press juice\n                    led to a progressive decrease of contraction strength, without jeopardising the\n                    vitality of myometrium strips. The bufadienolide-enriched fraction was the most\n                    active, since 1\u2009\u00b5g\/mL of the bufadienolide-enriched fraction lowered the area\n                    under the curve to 40.1\u2009\u00b1\u200911.8% of the initial value, whereas 150\u2009\u00b5g\/mL of the\n                    flavonoid-enriched fraction, 6.2\u2009\u00b5g\/mL of the flavonoid aglycon mixture, and\n                    10\u2009mg\/mL of the B. pinnatum leaf press juice were required to achieve\n                    comparable inhibition. A\u00a0progressive increase of contraction frequency was\n                    observed, except in the case of the flavonoid aglycon mixture, which did not\n                    affect frequency. None of the test substances decreased myometrial cell\n                    viability, even at concentrations of 500\u2009\u00b5g\/mL of the flavonoid-enriched\n                    fraction, 40\u2009\u00b5g\/mL of the flavonoid aglycon mixture, 3.8\u2009\u00b5g\/mL of the\n                    bufadienolide-enriched fraction, and 75\u2009mg\/mL of the B. pinnatum leaf\n                    press juice, i.e., higher than those used in the myometrium experiments. Given\n                    the concentrations of flavonoids in the flavonoid-enriched fraction and B.\n                        pinnatum leaf press juice, and of bufadienolides in the\n                    bufadienolide-enriched fraction and B. pinnatum leaf press juice, it\n                    appears that bufadienolides may be mainly responsible for the relaxant\n                    effect.<\/jats:p>","DOI":"10.1055\/a-0810-7704","type":"journal-article","created":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T23:56:50Z","timestamp":1545177410000},"page":"385-393","source":"Crossref","is-referenced-by-count":14,"title":["A Bufadienolide-Enriched Fraction of Bryophyllum pinnatum\n                    Inhibits Human Myometrial Contractility In Vitro"],"prefix":"10.1055","volume":"85","author":[{"given":"Stefanie","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, University Hospital Zurich, Zurich,\n                    Switzerland"},{"name":"Division of Pharmaceutical Biology, University of Basel, Basel,\n                    Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Haslinger","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, University Hospital Zurich, Zurich,\n                    Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristian","family":"Klaic","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, University Hospital Zurich, Zurich,\n                    Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria T.","family":"Faleschini","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutical Biology, University of Basel, Basel,\n                    Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nica","family":"Mennet","sequence":"additional","affiliation":[{"name":"Weleda AG, Arlesheim, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Potterat","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutical Biology, University of Basel, Basel,\n                    Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ursula","family":"von Mandach","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, University Hospital Zurich, Zurich,\n                    Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Hamburger","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutical Biology, University of Basel, Basel,\n                    Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula","family":"Sim\u00f5es-W\u00fcst","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, University Hospital Zurich, Zurich,\n                    Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2018,12,18]]},"reference":[{"key":"ref1","first-page":"20","article-title":"Bufadienolides and their medicinal utility: a review","volume":"5","author":"A Kamboj","year":"2013","journal-title":"Int J Pharm Pharm Sci"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.5897\/AJPP11.273","article-title":"Literature review on pharmacological potentials of Kalanchoe pinnata\n                    (Crassulaceae)","volume":"5","author":"S K Biswas","year":"2011","journal-title":"Afr J Pharm Pharmacol"},{"key":"ref3","first-page":"1359","article-title":"Bryophyllum pinnatum-reverse engineering of an anthroposophic herbal\n                    medicine","volume":"12","author":"M Hamburger","year":"2017","journal-title":"Nat Prod Commun"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1055\/s-0042-106727","article-title":"Bryophyllum pinnatum and related species used in anthroposophic medicine:\n                    constituents, pharmacological activities, and clinical efficacy","volume":"82","author":"K F\u00fcrer","year":"2016","journal-title":"Planta Med"},{"key":"ref5","first-page":"683","article-title":"Ein neuer Weg in der tokolytischen Therapie","volume":"34","author":"W Hassauer","year":"1985","journal-title":"Erfahrungsheilkunde"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1159\/000437154","article-title":"Die Anwendung von Bryophyllum pinnatum-Pr\u00e4paraten in der Geburtshilfe und\n                    Gyn\u00e4kologie \u2013 eine multizentrische prospektive Beobachtungsstudie","volume":"22","author":"K F\u00fcrer","year":"2015","journal-title":"Complement Med Res"},{"key":"ref7","first-page":"1","article-title":"Drugs in pregnancy and lactation \u2013 medications used in Swiss obstetrics","volume":"222","author":"L Schenkel","year":"2018","journal-title":"Z Geburtshilfe 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application","volume":"124","author":"N Plangger","year":"2006","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1159\/000487431","article-title":"Two randomised clinical trials on the use of Bryophyllum pinnatum in\n                    pre-term labour \u2013 results after discontinuation","volume":"25","author":"A P Sim\u00f5es-W\u00fcst","year":"2018","journal-title":"Complement Med Res"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1177\/1534735415580680","article-title":"Sleep quality improves during treatment with Bryophyllum pinnatum: an\n                    observational study on cancer patients","volume":"14","author":"A P Sim\u00f5es-W\u00fcst","year":"2015","journal-title":"Integr Cancer Ther"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0140-6736(12)61856-X","article-title":"Preventing preterm births: trends and potential reductions with current\n                    interventionsin 39 very high human development index countries","volume":"381","author":"H H Chang","year":"2013","journal-title":"Lancet"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1126\/science.1251816","article-title":"Preterm labor: one syndrome, many causes","volume":"345","author":"R Romero","year":"2014","journal-title":"Science"},{"key":"ref16","first-page":"227","article-title":"The prevention, diagnosis and treatment of premature labor","volume":"110","author":"E Schleu\u00dfner","year":"2013","journal-title":"Dtsch \u00c4rztebl Int"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"941057","DOI":"10.1155\/2011\/941057","article-title":"Prevention of preterm labour: 2011 update on tocolysis","volume":"2011","author":"C Hubinont","year":"2011","journal-title":"J Pregnancy"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"e155","DOI":"10.1097\/AOG.0000000000001711","article-title":"Practice Bulletin No.\u2009171: Management of preterm Labor","volume":"128","author":"ACOG: American college of Obstetricians and\n                            Gynecologists","year":"2016","journal-title":"Obstet Gynecol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/S0301-2115(03)00370-1","article-title":"Effect of Bryophyllum pinnatum versus fenoterol on uterine\n                    contractility","volume":"113","author":"B Gwehenberger","year":"2004","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.phymed.2011.06.032","article-title":"Leaf press juice from Bryophyllum pinnatum (Lamarck) Oken induces\n                    myometrial relaxation","volume":"19","author":"R W\u00e4chter","year":"2011","journal-title":"Phytomedicine"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1016\/j.phymed.2010.03.005","article-title":"Juice of Bryophyllum pinnatum (Lam.) inhibits oxytocin-induced increase\n                    of the intracellular calcium concentration in human myometrial cells","volume":"17","author":"A P Sim\u00f5es-W\u00fcst","year":"2010","journal-title":"Phytomedicine"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1055\/s-0033-1350808","article-title":"Two new flavonol glycosides and a metabolite profile of Bryophyllum\n                        pinnatum, a phytotherapeutic used in obstetrics and gynaecology","volume":"79","author":"K F\u00fcrer","year":"2013","journal-title":"Planta Med"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1055\/s-0035-1546126","article-title":"Quantification of bufadienolides in Bryophyllum pinnatum leaves and\n                    manufactured products by UHPLC-ESIMS\/MS","volume":"81","author":"M Oufir","year":"2015","journal-title":"Planta Med"},{"issue":"07","key":"ref24","first-page":"CD006169","article-title":"Combination of tocolytic agents for inhibiting preterm labour","author":"J P Vogel","year":"2014","journal-title":"Cochrane Database Syst Rev"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1055\/s-0043-109097","article-title":"Potential of Bryophyllum pinnatum as a detrusor relaxant: an in\n                        vitro exploratory study","volume":"83","author":"S Bachmann","year":"2017","journal-title":"Planta Med"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"10","DOI":"10.3945\/ajcn.116.136051","article-title":"The role of metabolism (and the microbiome) in defining the clinical efficacy of\n                    dietary flavonoids","volume":"105","author":"A Cassidy","year":"2017","journal-title":"Am J Clin Nutr"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1080\/19490976.2016.1158395","article-title":"Bacterial species involved in the conversion of dietary flavonoids in the human\n                    gut","volume":"7","author":"A Braune","year":"2016","journal-title":"Gut Microbes"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1007\/s11101-017-9525-1","article-title":"Bufadienolides of Kalanchoe species: an overview of chemical structure,\n                    biological activity and prospects for pharmacological use","volume":"16","author":"J Kolodziejczyk-Czepas","year":"2017","journal-title":"Phytochem Rev"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.phymed.2014.11.009","article-title":"Inhibition of porcine detrusor contractility by the flavonoid fraction of\n                        Bryophyllum pinnatum \u2013 a potential phytotherapeutic drug for the\n                    treatment of the overactive bladder syndrome","volume":"22","author":"K F\u00fcrer","year":"2015","journal-title":"Phytomedicine"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1095\/biolreprod67.2.506","article-title":"Telomerase immortalization of human myometrial cells","volume":"67","author":"J Condon","year":"2002","journal-title":"Biol Reprod"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"E1","DOI":"10.1152\/ajpendo.00529.2005","article-title":"Novel in vitro system for functional assessment of oxytocin action","volume":"292","author":"D Devost","year":"2007","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1006\/abbi.1994.1224","article-title":"Role of cellular energy status in tocopheryl hemisuccinate cytoprotection\n                    against ethyl methanesulfonate-induced toxicity","volume":"311","author":"S D Ray","year":"1994","journal-title":"Arch Biochem Biophys"}],"container-title":["Planta Medica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-0810-7704.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T22:51:33Z","timestamp":1655938293000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-0810-7704"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,18]]},"references-count":31,"journal-issue":{"issue":"05","published-online":{"date-parts":[[2019,3,15]]},"published-print":{"date-parts":[[2019,3]]}},"URL":"https:\/\/doi.org\/10.1055\/a-0810-7704","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0032-0943","1439-0221"],"issn-type":[{"value":"0032-0943","type":"print"},{"value":"1439-0221","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,18]]}}}