{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T22:47:50Z","timestamp":1770763670675,"version":"3.50.0"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"05","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Hibiscus sabdariffa is a medicinal plant consumed as a diuretic and anti-obesity remedy. Several pharmacological studies have shown its beneficial effects in metabolism. Peroxisome proliferator-activated receptors \u03b4 and \u03b3 may play a role in the actions of H. sabdariffa. These nuclear receptors regulate lipid and glucose metabolism and are therapeutic targets for type 2 diabetes. This research aimed to perform a phytochemical study guided by a bioassay from H. sabdariffa to identify compounds with peroxisome proliferator-activated receptor \u03b4 and peroxisome proliferator-activated receptor \u03b3 agonist activity, supported by messenger ribonucleic acid expression, molecular docking, lipid accumulation, and an antihyperglycemic effect. An oral glucose tolerance test in mice with the aqueous extract of H. sabdariffa and the dichloromethane extract of H.\u00a0sabdariffa was performed. The dichloromethane extract of H. sabdariffa exhibited an antihyperglycemic effect. The dichloromethane extract of H. sabdariffa was fractioned, and four fractions were evaluated in 3T3-L1 adipocytes on peroxisome proliferator-activated receptor \u03b4, peroxisome proliferator-activated receptor \u03b3, fatty acid transporter protein, and glucose transporter type 4 messenger ribonucleic acid expression. Fraction F3 exhibited peroxisome proliferator-activated receptor \u03b4\/\u03b3 dual agonist activity, and a further fractionation yielded two subfractions, F3-1 and F3-2, which also increased peroxisome proliferator-activated receptor \u03b4 and peroxisome proliferator-activated receptor \u03b3 expression. Subfractions were analyzed by GC\/MS. The main compounds identified in F3-1 were linoleic acid, oleic acid, and palmitic acid, while in F3-2, the main compounds identified were \u03b1-amyrin and lupeol. These molecules were subjected to molecular docking analysis. \u03b1-Amyrin and lupeol showed the highest affinity. Moreover, both produced an increase in peroxisome proliferator-activated receptor \u03b4, peroxisome proliferator-activated receptor \u03b3, fatty acid transporter protein, and glucose transporter type 4 expression. Additionally, \u03b1-amyrin and lupeol decreased lipid accumulation in 3T3-L1 adipocytes and blood glucose in mice. Until now, \u03b1-amyrin and lupeol have not been reported with activity on peroxisome proliferator-activated receptors. This study provides evidence that \u03b1-amyrin and lupeol possess antidiabetic effects through a peroxisome proliferator-activated receptor \u03b4\/\u03b3 dual agonist action.<\/jats:p>","DOI":"10.1055\/a-0824-1316","type":"journal-article","created":{"date-parts":[[2019,1,18]],"date-time":"2019-01-18T10:44:12Z","timestamp":1547808252000},"page":"412-423","source":"Crossref","is-referenced-by-count":30,"title":["Triterpenoids from Hibiscus sabdariffa L.\u00a0with PPAR\u03b4\/\u03b3 Dual Agonist Action: In Vivo, In Vitro and In Silico Studies"],"prefix":"10.1055","volume":"85","author":[{"given":"Abraham","family":"Giacoman-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Posgrado en Biolog\u00eda Experimental, Universidad Aut\u00f3noma Metropolitana-Iztapalapa, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Alarc\u00f3n-Aguilar","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda, Departamento de Ciencias de la Salud, Divisi\u00f3n de Ciencias Biol\u00f3gicas y de la Salud, Universidad Aut\u00f3noma Metropolitana-Iztapalapa, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Zamilpa","sequence":"additional","affiliation":[{"name":"Departamento de Fitoqu\u00edmica Farmacol\u00f3gica, Centro de Investigaci\u00f3n Biom\u00e9dica del Sur, Instituto Mexicano del Seguro Social, Xochitepec, Morelos, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Hidalgo-Figueroa","sequence":"additional","affiliation":[{"name":"CONACyT, IPICYT\/Consorcio de Investigaci\u00f3n, Innovaci\u00f3n y Desarrollo para las Zonas \u00c1ridas, San Luis Potos\u00ed, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Navarrete-V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia, Universidad Aut\u00f3noma del Estado de Morelos, Cuernavaca, Morelos, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebeca","family":"Garc\u00eda-Macedo","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Bioqu\u00edmica, Hospital de Especialidades, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rub\u00e9n","family":"Rom\u00e1n-Ramos","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda, Departamento de Ciencias de la Salud, Divisi\u00f3n de Ciencias Biol\u00f3gicas y de la Salud, Universidad Aut\u00f3noma Metropolitana-Iztapalapa, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio","family":"Almanza-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Laboratorio de Farmacolog\u00eda, Departamento de Ciencias de la Salud, Divisi\u00f3n de Ciencias Biol\u00f3gicas y de la Salud, Universidad Aut\u00f3noma Metropolitana-Iztapalapa, Ciudad de M\u00e9xico, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2019,1,16]]},"container-title":["Planta Medica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-0824-1316.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,3,16]],"date-time":"2019-03-16T01:09:53Z","timestamp":1552698593000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-0824-1316"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,16]]},"references-count":0,"journal-issue":{"issue":"05","published-online":{"date-parts":[[2019,3,15]]},"published-print":{"date-parts":[[2019,3]]}},"URL":"https:\/\/doi.org\/10.1055\/a-0824-1316","relation":{},"ISSN":["0032-0943","1439-0221"],"issn-type":[{"value":"0032-0943","type":"print"},{"value":"1439-0221","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,16]]}}}