{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,11,19]],"date-time":"2022-11-19T05:20:37Z","timestamp":1668835237012},"reference-count":32,"publisher":"Georg Thieme Verlag KG","issue":"05","funder":[{"name":"National Nature Science Foundation Grants of China","award":["No. 81370820"],"award-info":[{"award-number":["No. 81370820"]}]},{"name":"National Nature Science Foundation Grants of China","award":["No. 81400737"],"award-info":[{"award-number":["No. 81400737"]}]},{"name":"National Nature Science Foundation","award":["81370820"],"award-info":[{"award-number":["81370820"]}]},{"name":"National Nature Science Foundation","award":["81400737"],"award-info":[{"award-number":["81400737"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Clin Endocrinol Diabetes"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aims Our study aims to explore the role of \u03b2-catenin interaction protein-1(ICAT) in regulating peroxisome proliferator-activated receptor \u03b3 (PPAR\u03b3) transcriptional activity in mesangial cells. The abnormal ICAT expression in mesangial cells under high glucose(HG) contributes to the development of diabetes and its complications such as diabetic nephropathy (DN).<\/jats:p><jats:p>Methods Human mesangial cells (HMCs) were cultured in either 5.5 (normal control) or 30 (high glucose) mmol\/L glucose medium. Overexpression and knock-down of ICAT or \u03b2-catenin were carried out by transient transfection. PPAR\u03b3 transcriptional activity was evaluated by luciferase assay. Protein-protein interactions were tested by Coimmunoprecipitation and GST-pull down assay. Cell phenotype transition of HMCs was detected by the expression level of \u03b1-SMA and fibronectin, as well as MTT assay.<\/jats:p><jats:p>Results High \u03b2-catenin protein expression but low ICAT was accompanied by low PPAR\u03b3 transcriptional activity in HMCs cultured in HG. By using bioinformatics prediction, protein-protein and protein-DNA interaction experimental methods, ICAT and \u03b2-catenin were confirmed to act as coactivators in regulating PPAR\u03b3 transcriptional activity. Overexpression of ICAT could mitigate the decrease of PPAR\u03b3 transcriptional activity and partly relieve cell phenotype transition in HMCs.<\/jats:p><jats:p>Conclusions \u03b2-catenin and ICAT interact as coactivator to modulate PPAR\u03b3 transcriptional activation. In HMCs cultured in HG, the low expression of ICAT leads to low PPAR\u03b3 transcriptional activation.<\/jats:p>","DOI":"10.1055\/a-0879-1846","type":"journal-article","created":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T22:48:05Z","timestamp":1600296485000},"page":"365-373","source":"Crossref","is-referenced-by-count":0,"title":["ICAT acts as a Coactivator in Regulating PPAR\u03b3 Transcriptional Activity in Mesangial Cells"],"prefix":"10.1055","volume":"129","author":[{"given":"Yi","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Xinqiao Hospital, Third Military Medical University (Army Medical University), Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiawei","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Xinqiao Hospital, Third Military Medical University (Army Medical University), Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaopeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"General Hospital of Xingjiang Military, Xinjiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiao","family":"Mu","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Xinqiao Hospital, Third Military Medical University (Army Medical University), Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Xinqiao Hospital, Third Military Medical University (Army Medical University), Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Xinqiao Hospital, Third Military Medical University (Army Medical University), Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Xinqiao Hospital, Third Military Medical University (Army Medical University), Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2020,9,16]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1001\/jama.2013.168118","article-title":"Prevalence and control of diabetes in Chinese adults","volume":"310","author":"Y Xu","year":"2013","journal-title":"Jama"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1038\/nrneph.2017.63","article-title":"Reducing the costs of chronic kidney disease while delivering quality health care: A call to action","volume":"13","author":"R Vanholder","year":"2017","journal-title":"Nat Rev Nephrol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1097\/MNH.0b013e32834de526","article-title":"PPARgamma as a therapeutic target in diabetic nephropathy and other renal diseases","volume":"21","author":"J Yang","year":"2012","journal-title":"Curr Opin Nephrol Hypertens"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e0171474","DOI":"10.1371\/journal.pone.0171474","article-title":"Nephropathy in Pparg-null mice highlights PPARgamma systemic activities in metabolism and in the immune system","volume":"12","author":"B Toffoli","year":"2017","journal-title":"PLoS One"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.lfs.2010.06.004","article-title":"The combination of sirolimus and rosiglitazone produces a renoprotective effect on diabetic kidney disease in rats","volume":"87","author":"M Flaquer","year":"2010","journal-title":"Life Sci"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1042\/CS20070462","article-title":"Role of PPARgamma in renoprotection in Type 2 diabetes: Molecular mechanisms and therapeutic potential","volume":"116","author":"J Yang","year":"2009","journal-title":"Clin Sci (Lond)"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1038\/nrd4233","article-title":"Can we safely target the WNT pathway?","volume":"13","author":"M Kahn","year":"2014","journal-title":"Nat Rev Drug Discov"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1007\/978-90-481-3271-3_17","article-title":"Wnt signaling in pancreatic islets","volume":"654","author":"Z Liu","year":"2010","journal-title":"Adv Exp Med Biol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"987064","DOI":"10.1155\/2013\/987064","article-title":"A glimpse of the pathogenetic mechanisms of Wnt\/beta-catenin signaling in diabetic nephropathy","volume":"2013","author":"L Xiao","year":"2013","journal-title":"Biomed Res Int"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"e58622","DOI":"10.1371\/journal.pone.0058622","article-title":"Functional implications of microRNA-215 in TGF-beta1-induced phenotypic transition of mesangial cells by targeting CTNNBIP1","volume":"8","author":"J Mu","year":"2013","journal-title":"PLoS One"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1101\/gad.14.14.1741","article-title":"Inhibition of Wnt signaling by ICAT, a novel beta-catenin-interacting protein","volume":"14","author":"K Tago","year":"2000","journal-title":"Genes Dev"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1210\/me.2011-1023","article-title":"The beta-catenin binding protein ICAT modulates androgen receptor activity","volume":"25","author":"M Zhuo","year":"2011","journal-title":"Mol Endocrinol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"18567","DOI":"10.1073\/pnas.0509316102","article-title":"Repression of beta-catenin function in malignant cells by nonsteroidal antiinflammatory drugs","volume":"102","author":"D Lu","year":"2005","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"7137","DOI":"10.3748\/wjg.v20.i23.7137","article-title":"Emerging role of the beta-catenin-PPARgamma axis in the pathogenesis of colorectal cancer","volume":"20","author":"L Sabatino","year":"2014","journal-title":"World J Gastroenterol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"5827","DOI":"10.1128\/MCB.00441-06","article-title":"Functional interaction between peroxisome proliferator-activated receptor gamma and beta-catenin","volume":"26","author":"J Liu","year":"2006","journal-title":"Mol Cell Biol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"F916","DOI":"10.1152\/ajprenal.00697.2012","article-title":"ER stress triggers MCP-1 expression through SET7\/9-induced histone methylation in the kidneys of db\/db mice","volume":"306","author":"J Chen","year":"2014","journal-title":"Am J Physiol Renal Physiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1507\/endocrj.EJ12-0258","article-title":"Analysis of hepatic gene expression profile in a spontaneous mouse model of type 2 diabetes under a high sucrose diet","volume":"60","author":"K Nojima","year":"2013","journal-title":"Endocr J"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"673","DOI":"10.2215\/CJN.07891109","article-title":"Prevalence of chronic kidney disease in US adults with undiagnosed diabetes or prediabetes","volume":"5","author":"L C Plantinga","year":"2010","journal-title":"Clin J Am Soc Nephrol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1242\/dmm.009266","article-title":"Accelerated renal disease is associated with the development of metabolic syndrome in a glucolipotoxic mouse model","volume":"5","author":"C Martinez-Garcia","year":"2012","journal-title":"Dis Model Mech"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"308\u2013316","DOI":"10.1161\/HYPERTENSIONAHA.111.176263","article-title":"Telmisartan exerts renoprotective actions via peroxisome proliferator-activated receptor-gamma\/hepatocyte growth factor pathway independent of angiotensin II type 1 receptor blockade","volume":"59","author":"H Kusunoki","year":"2012","journal-title":"Hypertension"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0303-7207(98)00145-2","article-title":"Coactivators and corepressors as mediators of nuclear receptor function: An update","volume":"143","author":"G Jenster","year":"1998","journal-title":"Mol Cell Endocrinol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1146\/annurev.biochem.70.1.475","article-title":"Transcriptional coactivator complexes","volume":"70","author":"A M Naar","year":"2001","journal-title":"Annu Rev Biochem"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"14031","DOI":"10.1074\/jbc.M110.102855","article-title":"Activation of canonical wingless-type MMTV integration site family (Wnt) signaling in mature adipocytes increases beta-catenin levels and leads to cell dedifferentiation and insulin resistance","volume":"285","author":"B Gustafson","year":"2010","journal-title":"J Biol Chem"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"31553","DOI":"10.1038\/srep31553","article-title":"Adipose- and muscle-derived Wnts trigger pancreatic beta-cell adaptation to systemic insulin resistance","volume":"6","author":"K Kozinski","year":"2016","journal-title":"Sci Rep"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.2337\/db14-1164","article-title":"Noncanonical Wnt signaling promotes obesity-induced adipose tissue inflammation and metabolic dysfunction independent of adipose tissue expansion","volume":"64","author":"J J Fuster","year":"2015","journal-title":"Diabetes"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"e85479","DOI":"10.1371\/journal.pone.0085479","article-title":"The association of pioglitazone and urinary tract disease in type 2 diabetic Taiwanese: Bladder cancer and chronic kidney disease","volume":"9","author":"M Y Lee","year":"2014","journal-title":"PLoS One"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1177\/1479164116639229","article-title":"Linagliptin and pioglitazone combination therapy versus monotherapy with linagliptin or pioglitazone: A randomised, double-blind, parallel-group, multinational clinical trial","volume":"13","author":"M A Nauck","year":"2016","journal-title":"Diab Vasc Dis Res"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"4536","DOI":"10.1002\/jcb.26115","article-title":"Efficacy and safety of single- or double-drug antidiabetic regimens in the treatment of type 2 diabetes mellitus: A network meta-analysis","volume":"118","author":"X L Yang","year":"2017","journal-title":"J Cell Biochem"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"e7","DOI":"10.1038\/nutd.2011.3","article-title":"Metformin mitigates the impaired development of skeletal muscle in the offspring of obese mice","volume":"1","author":"J F Tong","year":"2011","journal-title":"Nutr Diabetes"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.mce.2011.04.003","article-title":"AMP-activated protein kinase attenuates Wnt\/beta-catenin signaling in human osteoblastic Saos-2 cells","volume":"339","author":"T Takatani","year":"2011","journal-title":"Mol Cell 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