{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T23:43:59Z","timestamp":1777419839954,"version":"3.51.4"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"06","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Horm Metab Res"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A rapid and fully automated chemiluminescent immunoassay for the detection of thyrotropin receptor autoantibodies (TSHR-Ab) based on a bridge technology was compared with two bioassays that measure either stimulating (TSAb) or blocking (TBAb) antibodies for the detection and differentiation of TSHR-Ab. A total of 229 patients with various thyroid disorders [151 with Graves\u2019 disease (GD), 35 with Hashimoto\u2019s thyroiditis (HT), 32 with nodular goiter, and 11 with thyroid cancer] were included. The bridge immunoassay was performed according to the manufacturer\u2019s instructions (cut-off&gt;0.55\u2009IU\/l). TSAb and TBAb were measured with reporter bioassays. Blocking activity was defined as percent inhibition of luciferase expression relative to induction with bovine TSH alone (cut-off&gt;34% inhibition). TSAb was reported as percentage of specimen-to-reference ratio (&gt;\u2009140 SRR%). The 3 TSHR-Ab assays were negative in all patients with benign euthyroid nodular goiter and differentiated thyroid cancer. In contrast, in all patients with GD, irrespective of the disease duration, TSHR-Ab positivity was present in 127 of 151 (84%) and 140 (93%) for the bridge assay and TSAb bioassay, respectively (p&lt;0.001). Fifteen of 151 (10%) GD samples were positive in the TSAb bioassay but negative in the bridge assay. The bridge assay and the TSAb bioassay correlated positively (r=0.39, p&lt;0.0001) in patients with GD. Both assays detected TSHR-Ab in all ten untreated hyperthyroid patients with GD. In GD patients with a duration of less than six months, 27\/29 (93%) and 28 (97%) were TSHR-Ab positive with the bridge and TSAb bioassay, respectively. In comparison, TSHR-Ab were present in two of 35 (6%) and five (14%) HT patients with the bridge and TSAb bio-assay, respectively. TSHR blocking antibodies were present in one (3%) patient with HT and in two (1%) patients with GD; these two GD patients were also bridge assay positive but TSAb bioassay negative. In conclusion, the bridge immunoassay and both bioassays are highly sensitive for the detection of TSHR-Ab. The bridge assay is, however, also positive in the presence of TSHR blocking antibodies detected in a TBAb bioassay.<\/jats:p>","DOI":"10.1055\/a-0914-0535","type":"journal-article","created":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T22:31:10Z","timestamp":1560810670000},"page":"341-346","source":"Crossref","is-referenced-by-count":35,"title":["Comparison of a Bridge Immunoassay with Two Bioassays for Thyrotropin Receptor Antibody Detection and Differentiation"],"prefix":"10.1055","volume":"51","author":[{"given":"Stephanie","family":"Allelein","sequence":"additional","affiliation":[{"name":"Medical Faculty, Division for Specific Endocrinology, University of D\u00fcsseldorf, D\u00fcsseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanja","family":"Diana","sequence":"additional","affiliation":[{"name":"Department of Medicine I, Molecular Thyroid Research Laboratory, Johannes Gutenberg University (JGU) Medical Center, Mainz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margret","family":"Ehlers","sequence":"additional","affiliation":[{"name":"Medical Faculty, Division for Specific Endocrinology, University of D\u00fcsseldorf, D\u00fcsseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Kanitz","sequence":"additional","affiliation":[{"name":"Department of Medicine I, Molecular Thyroid Research Laboratory, Johannes Gutenberg University (JGU) Medical Center, Mainz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derik","family":"Hermsen","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry and Laboratory Diagnostics, Medical Faculty, University of D\u00fcsseldorf, D\u00fcsseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Schott","sequence":"additional","affiliation":[{"name":"Medical Faculty, Division for Specific Endocrinology, University of D\u00fcsseldorf, D\u00fcsseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George J.","family":"Kahaly","sequence":"additional","affiliation":[{"name":"Department of Medicine I, Molecular Thyroid Research Laboratory, Johannes Gutenberg University (JGU) Medical Center, Mainz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2019,6,17]]},"container-title":["Hormone and Metabolic Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-0914-0535.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T22:31:11Z","timestamp":1560810671000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-0914-0535"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6]]},"references-count":0,"journal-issue":{"issue":"06","published-online":{"date-parts":[[2019,6,17]]},"published-print":{"date-parts":[[2019,6]]}},"URL":"https:\/\/doi.org\/10.1055\/a-0914-0535","relation":{},"ISSN":["0018-5043","1439-4286"],"issn-type":[{"value":"0018-5043","type":"print"},{"value":"1439-4286","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6]]}}}