{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T21:55:03Z","timestamp":1766181303810},"reference-count":35,"publisher":"Georg Thieme Verlag KG","issue":"09","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Klin Monbl Augenheilkd"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Zusammenfassung<\/jats:title><jats:p>Hintergrund In den letzten Jahren mehren sich Hinweise auf eine entz\u00fcndliche Komponente beim Keratokonus (KC). Ein Schl\u00fcsselgen bei entz\u00fcndlichen Prozessen ist der Nuclear Factor Kappa B (NF-\u03baB). NF-\u03baB ist ein Transkriptionsfaktor, der unter anderem das Enzym Nitric Oxide Synthase (NOS), das mit dem konkurrierenden Enzym Arginase (Arg) bei entz\u00fcndlichen Prozessen involviert ist, aktiviert. Ziel dieser Studie war es, die Isotypen von NOS und Arginase zu analysieren, die Expression NF-\u03baB, NOS und Arginase sowie den regulativen Mechanismus von NOS und Arginase in Keratozyten von Keratokonuspatienten mithilfe des Inhibitors 1400W in vitro zu untersuchen.<\/jats:p><jats:p>Methoden Prim\u00e4re humane Keratozyten wurden durch enzymatische Behandlung mit Kollagenase A aus humanen Korneoskleralscheiben (n\u2009=\u20098) und von Explantaten von geplanten perforierenden Keratoplastiken (KC-Patienten) isoliert (n\u2009=\u20098) und in DMEM\/F12-Kulturmedium, versetzt mit 5% fetalem K\u00e4lberserum, kultiviert. Die Expression von NF-\u03baB, NOS und Arginase wurden mit quantitativer PCR (qPCR) und Westernblot-Analyse (WB) untersucht. Nitrit- und Ureakonzentrationen im Zellkultur\u00fcberstand wurden nach Zugabe des NOS-Inhibitors 1400W (0\u200a\u2013\u200a40\u2009\u00b5M) analysiert.<\/jats:p><jats:p>Ergebnisse In den Keratozyten wurden ausschlie\u00dflich die Isotypen iNOS (induzierbare NO-Synthase) und Arg-II nachgewiesen. Die mRNA-Expression von NF-\u03baB und iNOS waren in KC-Keratozyten h\u00f6her als in normalen Zellen (p\u2009=\u20090,0135 und p\u2009=\u20090,0001), w\u00e4hrend in der Arg-II-Expression keine Unterschiede messbar waren. Im WB war bei NF-\u03baB eine h\u00f6here Bandenintensit\u00e4t messbar (p\u2009=\u20090,0012), bei iNOS konnten keine Unterschiede in der Bandenintensit\u00e4t nachgewiesen werden. Im \u00dcberstand der KC-Keratozyten wurden geringere Konzentrationen von Nitrit und Urea nach Zugabe des Inhibitors 1400W gemessen (p\u2009=\u2009\u2264\u20090,014), nicht jedoch bei normalen Zellen (p\u2009\u2265\u20090,178).<\/jats:p><jats:p>Schlussfolgerung Aufgrund der erh\u00f6hten Expression von NF-\u03baB und iNOS muss von einer inflammatorischen Komponente beim Keratokonus ausgegangen werden. Die unterschiedliche Regulation der KC-Keratozyten durch den iNOS-Inhibitor 1400W legt eine ver\u00e4nderte metabolische Aktivit\u00e4t nahe, die durch entz\u00fcndliche Prozesse hervorgerufen werden kann.<\/jats:p>","DOI":"10.1055\/a-1002-0100","type":"journal-article","created":{"date-parts":[[2019,10,10]],"date-time":"2019-10-10T23:06:07Z","timestamp":1570748767000},"page":"1010-1017","source":"Crossref","is-referenced-by-count":11,"title":["Erh\u00f6hte NF-\u03baB- und iNOS-Expression in Keratozyten von Keratokonuspatienten \u2013 Hinweise auf eine entz\u00fcndliche Komponente?"],"prefix":"10.1055","volume":"238","author":[{"given":"Tanja","family":"Stachon","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Universit\u00e4tsklinikum des Saarlandes UKS, Homburg\/Saar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenz","family":"Latta","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Universit\u00e4tsklinikum des Saarlandes UKS, Homburg\/Saar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krasimir","family":"Kolev","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Semmelweis University, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Berthold","family":"Seitz","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Universit\u00e4tsklinikum des Saarlandes UKS, Homburg\/Saar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Achim","family":"Langenbucher","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Experimentelle Ophthalmologie, Universit\u00e4t des Saarlandes, Homburg\/Saar"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N\u00f3ra","family":"Szentm\u00e1ry","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Universit\u00e4tsklinikum des Saarlandes UKS, Homburg\/Saar"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2019,10,10]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/0039-6257(84)90094-8","article-title":"Keratoconus and related noninflammatory corneal thinning disorders","volume":"28","author":"J H Krachmer","year":"1984","journal-title":"Surv Ophthalmol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.ajo.2016.12.015","article-title":"Age-specific Incidence and Prevalence of Keratoconus: A Nationwide Registration Study","volume":"175","author":"D A Godefrooij","year":"2017","journal-title":"Am J Ophthalmol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1111\/j.1442-9071.2005.00941.x","article-title":"Computerized corneal topography in a paediatric population with Down syndrome","volume":"33","author":"A L Vincent","year":"2005","journal-title":"Clin Experiment 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