{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T02:59:42Z","timestamp":1785812382209,"version":"3.56.0"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"04","funder":[{"name":"Silvana Legnani"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Infections caused by HSV-2 are a public health concern worldwide, and there is still a great demand for the discovery of novel anti-herpes virus agents effective against strains resistant to current antiviral agents. In this context, medicinal plants represent an alternative source of active compounds for developing efficient antiviral therapies. The aim of this study was to evaluate the antiviral activity of Arisaema tortuosum, a plant used in the traditional medicine of India. A chloroform soluble fraction of the leaves exhibited anti-HSV-2 activity with a selectivity index of 758. The extract was also active against acyclovir-resistant HSV-2 and HSV-1. The mechanism of action of the extract was investigated evidencing inhibition of both early and late events of the HSV-2 replicative cycle. A HPLC-PDA-MS\/MS analysis showed the presence of flavonoids including apigenin and luteolin in the chloroform extract (CE). Apigenin and luteolin showed a high inhibitory activity with EC50 values of 0.05 and 0.41\u2009\u00b5g\/mL, respectively. Both compounds exhibited antiviral activity when added up to 6\u2009h post infection and were able to reduce the viral progeny production. In addition, apigenin interfered with cell-to-cell virus spread.<\/jats:p>","DOI":"10.1055\/a-1087-8303","type":"journal-article","created":{"date-parts":[[2020,1,23]],"date-time":"2020-01-23T00:23:12Z","timestamp":1579738992000},"page":"267-275","source":"Crossref","is-referenced-by-count":33,"title":["Antiviral Activity of a Arisaema Tortuosum Leaf Extract and Some of its Constituents against Herpes Simplex Virus Type 2"],"prefix":"10.1055","volume":"86","author":[{"given":"Massimo","family":"Ritt\u00e0","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, University of Torino, Orbassano, Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arianna","family":"Marengo","sequence":"additional","affiliation":[{"name":"Department of Drug Science and Technology, University of Torino, Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Civra","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, University of Torino, Orbassano, Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Lembo","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, University of Torino, Orbassano, Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Cagliero","sequence":"additional","affiliation":[{"name":"Department of Drug Science and Technology, University of Torino, Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamal","family":"Kant","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences & Technology, Birla Institute of Technology, Mesra, Ranchi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uma Ranjan","family":"Lal","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences & Technology, Birla Institute of Technology, Mesra, Ranchi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Rubiolo","sequence":"additional","affiliation":[{"name":"Department of Drug Science and Technology, University of Torino, Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manik","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences & Technology, Birla Institute of Technology, Mesra, Ranchi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Donalisio","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, University of Torino, Orbassano, Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2020,1,22]]},"container-title":["Planta Medica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-1087-8303.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T23:31:23Z","timestamp":1583364683000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-1087-8303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,22]]},"references-count":0,"journal-issue":{"issue":"04","published-online":{"date-parts":[[2020,3,4]]},"published-print":{"date-parts":[[2020,3]]}},"URL":"https:\/\/doi.org\/10.1055\/a-1087-8303","relation":{},"ISSN":["0032-0943","1439-0221"],"issn-type":[{"value":"0032-0943","type":"print"},{"value":"1439-0221","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,22]]}}}