{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:19:34Z","timestamp":1790669974965,"version":"4.1.0"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"04","funder":[{"name":"CHU Robert Debr\u00e9 Reims","award":["Co-funding PhD thesis"],"award-info":[{"award-number":["Co-funding PhD thesis"]}]},{"DOI":"10.13039\/501100002334","name":"Conseil R\u00e9gional Champagne Ardenne","doi-asserted-by":"crossref","award":["co-funding PhD thesis"],"award-info":[{"award-number":["co-funding PhD thesis"]}],"id":[{"id":"10.13039\/501100002334","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Soci\u00e9t\u00e9 d'Acc\u00e9l\u00e9ration du Transfert de Technologie Nord"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Toxoplasma gondii, belonging to the Apicomplexa phylum, is a cosmopolitan protozoan parasite that affects at least 30% of the world\u02bcs population. In West Africa, the leaves and bark of the tree species Anogeissus leiocarpa (DC.) Guill. &amp; Perr. are used against zoonosis in traditional medicine and play a key role in controlling diseases induced by Apicomplexans such as malaria. In this study, extracts, fractions, and pure compounds obtained from an ethanol extract of the bark of A.\u00a0leiocarpa were evaluated against T. gondii infection in vitro and in vivo. The crude bark extract showed significant activity on tachyzoites from the T. gondii RH strain (IC50\u2009=\u200959.30\u2009\u00b5g\/mL). The crude bark extract without tannins and pure trachelosperogenin E purified by centrifugal partition chromatography showed the highest activity (IC50s\u2009=\u200912.83 and 26.63\u2009\u00b5g\/mL, respectively) with satisfying selectivity indexes of 9.61 and 9.75, respectively. The crude bark extract without tannins and pure trachelosperogenin E were able to significantly inhibit host cell invasion by the parasite in vitro, while the crude bark extract without tannins was able to increase mice survival in our murine model of chronic toxoplasmosis. These results provide new biological data for natural compounds that could enhance the current panoply of treatments against toxoplasmosis.<\/jats:p>","DOI":"10.1055\/a-1088-8449","type":"journal-article","created":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T01:14:20Z","timestamp":1580260460000},"page":"294-302","source":"Crossref","is-referenced-by-count":7,"title":["In Vitro and In Vivo Activity of Anogeissus leiocarpa Bark Extract and Isolated Metabolites against Toxoplasma gondii"],"prefix":"10.1055","volume":"86","author":[{"given":"J\u00e9r\u00e9my","family":"Spalenka","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Reims Champagne Ardenne, CNRS, ICMR UMR 7312, Reims, France"},{"name":"Universit\u00e9 de Reims Champagne Ardenne, ESCAPE EA 7510, Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Hubert","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Reims Champagne Ardenne, CNRS, ICMR UMR 7312, Reims, France"},{"name":"NatExplore SAS, Prouilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Voutquenne-Nazabadioko","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Reims Champagne Ardenne, CNRS, ICMR UMR 7312, Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandie","family":"Escotte-Binet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Reims Champagne Ardenne, ESCAPE EA 7510, Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Borie","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Reims Champagne Ardenne, CNRS, ICMR UMR 7312, Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Velard","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Reims Champagne Ardenne, BIOS, EA 4691, Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Villena","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Reims Champagne Ardenne, ESCAPE EA 7510, Reims, France"},{"name":"Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, CHU H\u00f4pital Maison Blanche, Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Aubert","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Reims Champagne Ardenne, ESCAPE EA 7510, Reims, France"},{"name":"Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, CHU H\u00f4pital Maison Blanche, Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Hugues","family":"Renault","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Reims Champagne Ardenne, CNRS, ICMR UMR 7312, Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2020,1,28]]},"container-title":["Planta Medica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-1088-8449.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T23:31:30Z","timestamp":1583364690000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-1088-8449"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,28]]},"references-count":0,"journal-issue":{"issue":"04","published-online":{"date-parts":[[2020,3,4]]},"published-print":{"date-parts":[[2020,3]]}},"URL":"https:\/\/doi.org\/10.1055\/a-1088-8449","relation":{},"ISSN":["0032-0943","1439-0221"],"issn-type":[{"value":"0032-0943","type":"print"},{"value":"1439-0221","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,28]]}}}