{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:24:18Z","timestamp":1790641458788,"version":"4.1.0"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"04","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Kalanchoe species are well-known medicinal plants used in traditional medicine as anti-inflammatory and analgesic remedies. Recently, it has been reported that Kalanchoe plants have cytotoxic properties; however, data on traditional use of these plants in tumor treatment are extremely limited. Kalanchoe daigremontiana is one of the most popular species cultivated in Europe, and it is used, among other things, as a remedy in treating skin injuries and wounds. Studies on the biological activity of this species are scarce, and there is a lack of data on the cytotoxic activity of K. daigremontiana extracts on epithelial cancer cells in the literature. In our present study, we analyzed the phytochemical composition of K. daigremontiana ethanol extract and fractions\u2013water and dichloromethane\u2013by the HPLC-DAD-ESI-MS method and estimated cytotoxic activity of the extracts on human adenocarcinoma (HeLa), ovarian (SKOV-3), breast (MCF-7), and melanoma (A375) cell lines by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, real-time cell analyzer (RTCA), and flow cytometry. We identified 6 bufadienolide compounds and 19 flavonoids, mostly kaempferol, quercetin, isorhamnetin, and myricetin glycosides, of which only 3 flavonoids have been identified in K. daigremontiana to date. Other flavonoids that were characterized in our study have not yet been found in this plant. The ethanol extract and water fraction of K. daigremontiana did not show significant cytotoxic activity on the tested cell lines. In contrast, the dichloromethane fraction showed the strongest activity against all cell lines with IC50 values of \u2264\u200910\u2009\u00b5g\/mL. The results indicated that this activity is mainly due to the presence of bersaldegenin-1,3,5-orthoacetate.<\/jats:p>","DOI":"10.1055\/a-1099-9786","type":"journal-article","created":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T01:14:20Z","timestamp":1580260460000},"page":"239-246","source":"Crossref","is-referenced-by-count":30,"title":["Identification of Flavonoids and Bufadienolides and Cytotoxic Effects of Kalanchoe daigremontiana Extracts on Human Cancer Cell Lines"],"prefix":"10.1055","volume":"86","author":[{"given":"Justyna","family":"Stefanowicz-Hajduk","sequence":"additional","affiliation":[{"name":"Department of Biology and Pharmaceutical Botany, Medical University of Gda\u0144sk, Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Asztemborska","sequence":"additional","affiliation":[{"name":"Institute of Physical Chemistry of the Polish Academy of Sciences, Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miros\u0142awa","family":"Krauze-Baranowska","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Medical University of Gda\u0144sk, Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylwia","family":"Godlewska","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Medical University of Gda\u0144sk, Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Gucwa","sequence":"additional","affiliation":[{"name":"Department of Biology and Pharmaceutical Botany, Medical University of Gda\u0144sk, Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Moniuszko-Szajwaj","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, Pu\u0142awy, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Stochmal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, Pu\u0142awy, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Renata","family":"Ochocka","sequence":"additional","affiliation":[{"name":"Department of Biology and Pharmaceutical Botany, Medical University of Gda\u0144sk, Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2020,1,28]]},"container-title":["Planta Medica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-1099-9786.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T23:31:13Z","timestamp":1583364673000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-1099-9786"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,28]]},"references-count":0,"journal-issue":{"issue":"04","published-online":{"date-parts":[[2020,3,4]]},"published-print":{"date-parts":[[2020,3]]}},"URL":"https:\/\/doi.org\/10.1055\/a-1099-9786","relation":{},"ISSN":["0032-0943","1439-0221"],"issn-type":[{"value":"0032-0943","type":"print"},{"value":"1439-0221","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,28]]}}}