{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T22:22:38Z","timestamp":1785882158729,"version":"3.56.0"},"reference-count":37,"publisher":"Georg Thieme Verlag KG","issue":"10","funder":[{"name":"Deutsche Forschungsgemeinschaft (DFG) within the CRC\/TRR\n                        205 \u201cThe adrenal, central relay in health and disease\u201d,\n                        project B16 to MKr and MF","award":["KR4371\/1\u20132 and FA 466\/4\u20132 to MKr and\n                        MF"],"award-info":[{"award-number":["KR4371\/1\u20132 and FA 466\/4\u20132 to MKr and\n                        MF"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Clin Endocrinol Diabetes"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n                    Context NCI-H295 cells are the most widely used model for adrenal\n                    steroidogenesis and adrenocortical carcinoma and have been used for decades in\n                    laboratories worldwide. However, reported steroidogenic properties differ\n                    considerably.<\/jats:p><jats:p>\n                    Objective To evaluate heterogeneity of steroidogenesis among NCI-H295\n                    cell strains, clarify the influence of culture media and test response to\n                    inhibitors of steroidogenesis by using liquid chromatography tandem mass\n                    spectrometry (LC-MS\/MS).<\/jats:p><jats:p>\n                    Methods NCI-H295 cells were obtained from two cell banks and cultivated\n                    in different media. An LC-MS\/MS-based panel analysis of thirteen\n                    steroids was adapted for cell culture supernatant. Cells were treated with\n                    metyrapone, abiraterone and mitotane.<\/jats:p><jats:p>\n                    Results Mineralocorticoid synthesis was strongly affected by passaging as\n                    reflected by reduction of aldosterone secretion from 0.158\u00b10.006 to\n                        0.017\u00b10.001\u2009\u00b5g\/106 cells\n                    (p&lt;0.05). Relevant differences were also found for cells from two\n                    vendors in terms of aldosterone secretion (0.180\u00b10.001 vs.\n                        0.09\u00b10.002\u2009\u00b5g\/106 cells,\n                    p&lt;0.05). Selection of medium strongly impacted on cortisol secretion\n                    with&gt;4-fold difference (40.6\u00b15.5 vs.\n                        182.1\u00b123\u2009\u00b5g\/106 cells) and\n                    reflected differential activation of the glucocorticoid pathway. Exposure to\n                    abiraterone, metyrapone and mitotane resulted in characteristic steroidogenic\n                    profiles consistent with known mechanism of drug action with considerable\n                    differences in metabolites upstream of the blocked enzyme.<\/jats:p><jats:p>\n                    Conclusion We demonstrate that steroid hormone secretion in NCI-H295\n                    cells is strongly affected by the individual strain, passage and growing\n                    conditions. These factors should be taken into account in the evaluation of\n                    experiments analyzing steroid parameters directly or as surrogate parameters of\n                    cell viability.<\/jats:p>","DOI":"10.1055\/a-1105-6332","type":"journal-article","created":{"date-parts":[[2020,4,29]],"date-time":"2020-04-29T23:14:01Z","timestamp":1588202041000},"page":"672-680","source":"Crossref","is-referenced-by-count":26,"title":["Steroidogenesis in the NCI-H295 Cell Line Model is Strongly Affected\n                    By Culture Conditions and Substrain"],"prefix":"10.1055","volume":"128","author":[{"given":"Max","family":"Kurlbaum","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Division of Endocrinology and\n                    Diabetes, University of W\u00fcrzburg, University Hospital W\u00fcrzburg,\n                    W\u00fcrzburg, Germany"},{"name":"University Hospital W\u00fcrzburg, Central Laboratory,\n                    W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silviu","family":"Sbiera","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Division of Endocrinology and\n                    Diabetes, University of W\u00fcrzburg, University Hospital W\u00fcrzburg,\n                    W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Kendl","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Division of Endocrinology and\n                    Diabetes, University of W\u00fcrzburg, University Hospital W\u00fcrzburg,\n                    W\u00fcrzburg, Germany"},{"name":"University Hospital W\u00fcrzburg, Central Laboratory,\n                    W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Martin Fassnacht","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Division of Endocrinology and\n                    Diabetes, University of W\u00fcrzburg, University Hospital W\u00fcrzburg,\n                    W\u00fcrzburg, Germany"},{"name":"University Hospital W\u00fcrzburg, Central Laboratory,\n                    W\u00fcrzburg, Germany"},{"name":"Comprehensive Cancer Center Mainfranken, University of\n                    W\u00fcrzburg, W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Kroiss","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Division of Endocrinology and\n                    Diabetes, University of W\u00fcrzburg, University Hospital W\u00fcrzburg,\n                    W\u00fcrzburg, Germany"},{"name":"Comprehensive Cancer Center Mainfranken, University of\n                    W\u00fcrzburg, W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2020,4,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1853","DOI":"10.1210\/jc.2008-2291","article-title":"Clinical review: Prevalence and incidence of endocrine and metabolic disorders\n                    in the United States: A comprehensive review","volume":"94","author":"S H Golden","year":"2009","journal-title":"J Clin Endocrinol Metab"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2013.02.034","article-title":"Adrenocortical carcinoma: A population-based study on incidence and survival in\n                the Netherlands since 1993","author":"T M Kerkhofs","year":"2013","journal-title":"Eur J Cancer"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"4551","DOI":"10.1210\/jc.2013-3020","article-title":"Update in adrenocortical carcinoma","volume":"98","author":"M Fassnacht","year":"2013","journal-title":"J Clin Endocrinol Metab"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1210\/er.2013-1029","article-title":"Adrenocortical carcinoma","volume":"35","author":"T Else","year":"2014","journal-title":"Endocrine reviews"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"2119","DOI":"10.1093\/annonc\/mdv329","article-title":"Prognostic factors in stage III-IV adrenocortical carcinomas (ACC): An European\n                    Network for the Study of Adrenal Tumor (ENSAT) study","volume":"26","author":"R Libe","year":"2015","journal-title":"Annals of oncology: Official journal of the European Society for\n                    Medical Oncology \/ ESMO"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"49636","DOI":"10.18632\/oncotarget.10421","article-title":"Metformin as a new anti-cancer drug in adrenocortical carcinoma","volume":"7","author":"G Poli","year":"2016","journal-title":"Oncotarget"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1775","DOI":"10.1210\/en.2015-2052","article-title":"ATR-101, a Selective and Potent Inhibitor of Acyl-CoA Acyltransferase 1,\n                    Induces Apoptosis in H295R Adrenocortical Cells and in the Adrenal Cortex of\n                    Dogs","volume":"157","author":"C R LaPensee","year":"2016","journal-title":"Endocrinology"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1007\/s12672-016-0273-2","article-title":"Drug Synergism of Proteasome Inhibitors and Mitotane by Complementary Activation\n                    of ER Stress in Adrenocortical Carcinoma Cells","volume":"7","author":"M Kroiss","year":"2016","journal-title":"Horm Cancer"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"3895","DOI":"10.1210\/en.2015-1367","article-title":"Mitotane Inhibits Sterol-O-Acyl Transferase 1 Triggering Lipid-Mediated\n                    Endoplasmic Reticulum Stress and Apoptosis in Adrenocortical Carcinoma\n                    Cells","volume":"156","author":"S Sbiera","year":"2015","journal-title":"Endocrinology"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.mce.2013.08.023","article-title":"Cytotoxic activity of gemcitabine, alone or in combination with mitotane, in\n                    adrenocortical carcinoma cell lines","volume":"382","author":"A Germano","year":"2014","journal-title":"Molecular and cellular endocrinology"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1530\/ERC-11-0337","article-title":"Combination of sorafenib and everolimus impacts therapeutically on\n                    adrenocortical tumor models","volume":"19","author":"B Mariniello","year":"2012","journal-title":"Endocr Relat Cancer"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"41220","DOI":"10.1074\/jbc.M204405200","article-title":"The expression of novH in adrenocortical cells is down-regulated by TGFbeta 1\n                    through c-Jun in a Smad-independent manner","volume":"277","author":"J Lafont","year":"2002","journal-title":"The Journal of biological chemistry"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"E1480","DOI":"10.1152\/ajpendo.90652.2008","article-title":"Both TASK-3 and TREK-1 two-pore loop K channels are expressed in H295R cells and\n                    modulate their membrane potential and aldosterone secretion","volume":"295","author":"T Brenner","year":"2008","journal-title":"American journal of physiology. Endocrinology and\n                    metabolism"},{"key":"ref14","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.aquatox.2011.11.006","article-title":"Disruption of endocrine function in in vitro H295R cell-based and in in vivo\n                    assay in zebrafish by 2,4-dichlorophenol","volume":"106-107","author":"Y Ma","year":"2012","journal-title":"Aquat Toxicol"},{"key":"ref15","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1093\/toxsci\/kfw002","article-title":"High-Throughput Screening of Chemical Effects on Steroidogenesis Using H295R\n                    Human Adrenocortical Carcinoma Cells","volume":"150","author":"A L Karmaus","year":"2016","journal-title":"Toxicological sciences: An official journal of the Society of\n                    Toxicology"},{"key":"ref16","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.taap.2007.07.013","article-title":"Modulation of steroidogenic gene expression and hormone production of H295R\n                    cells by pharmaceuticals and other environmentally active compounds","volume":"225","author":"T Gracia","year":"2007","journal-title":"Toxicology and applied pharmacology"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1016\/j.taap.2006.05.007","article-title":"In vitro effects of brominated flame retardants and metabolites on CYP17\n                    catalytic activity: A novel mechanism of action?","volume":"216","author":"R F Canton","year":"2006","journal-title":"Toxicology and applied pharmacology"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1055\/s-2004-861550","article-title":"The rise in adrenal androgen biosynthesis: Adrenarche","volume":"22","author":"J C Havelock","year":"2004","journal-title":"Semin Reprod Med"},{"key":"ref19","doi-asserted-by":"publisher","first-page":"79292","DOI":"10.18632\/oncotarget.12685","article-title":"Targeting heterogeneity of adrenocortical carcinoma: Evaluation and extension of\n                    preclinical tumor models to improve clinical translation","volume":"7","author":"C Hantel","year":"2016","journal-title":"Oncotarget"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1530\/ERC-17-0447","article-title":"Development of new preclinical models to advance adrenocortical carcinoma\n                    research","volume":"25","author":"K Kiseljak-Vassiliades","year":"2018","journal-title":"Endocr Relat Cancer"},{"key":"ref21","first-page":"5488","article-title":"Establishment and characterization of a human adrenocortical carcinoma cell line\n                    that expresses multiple pathways of steroid biosynthesis","volume":"50","author":"A F Gazdar","year":"1990","journal-title":"Cancer Res"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.mce.2011.08.041","article-title":"Human adrenocortical carcinoma cell lines","volume":"351","author":"T Wang","year":"2012","journal-title":"Mol Cell Endocrinol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"4542","DOI":"10.1210\/jc.2008-0903","article-title":"Development of an adrenocorticotropin-responsive human adrenocortical carcinoma\n                    cell line","volume":"93","author":"J Parmar","year":"2008","journal-title":"J Clin Endocrinol Metab"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0303-7207(94)90277-1","article-title":"The NCI-H295 cell line: a pluripotent model for human adrenocortical\n                    studies","volume":"100","author":"W E Rainey","year":"1994","journal-title":"Mol Cell Endocrinol"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1055\/s-0032-1323810","article-title":"Characterization of NCI-H295R cells as an in vitro model of\n                    hyperaldosteronism","volume":"45","author":"U D Lichtenauer","year":"2013","journal-title":"Horm Metab Res"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1677\/JOE-07-0166","article-title":"Human adrenal corticocarcinoma NCI-H295R cells produce more androgens than\n                    NCI-H295A cells and differ in 3beta-hydroxysteroid dehydrogenase type 2 and\n                    17,20 lyase activities","volume":"195","author":"E Samandari","year":"2007","journal-title":"J Endocrinol"},{"key":"ref27","doi-asserted-by":"publisher","first-page":"1555","DOI":"10.1210\/endo.133.4.8404594","article-title":"Human NCI-H295 adrenocortical carcinoma cells: a model for\n                    angiotensin-II-responsive aldosterone secretion","volume":"133","author":"I M Bird","year":"1993","journal-title":"Endocrinology"},{"key":"ref28","doi-asserted-by":"publisher","first-page":"932","DOI":"10.1210\/endo.132.2.8381079","article-title":"Angiotensin-II stimulates an increase in cAMP and expression of 17\n                    alpha-hydroxylase cytochrome P450 in fetal bovine adrenocortical cells","volume":"132","author":"I M Bird","year":"1993","journal-title":"Endocrinology"},{"key":"ref29","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1210\/jcem.77.3.8396576","article-title":"Regulation of human adrenal carcinoma cell (NCI-H295) production of C19\n                    steroids","volume":"77","author":"W E Rainey","year":"1993","journal-title":"J Clin Endocrinol Metab"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"2012","DOI":"10.1210\/endo-122-5-2012","article-title":"Dual regulation of 3 beta-hydroxysteroid dehydrogenase, 17 alpha-hydroxylase,\n                    and dehydroepiandrosterone sulfotransferase by adenosine\n                    3',5'-monophosphate and activators of protein kinase C in\n                    cultured human adrenocortical cells","volume":"122","author":"J M McAllister","year":"1988","journal-title":"Endocrinology"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1038\/s41586-018-0409-3","article-title":"Genetic and transcriptional evolution alters cancer cell line drug response","volume":"560","author":"U Ben-David","year":"2018","journal-title":"Nature"},{"key":"ref32","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1055\/s-0031-1298019","article-title":"Comparison of aldosterone production among human adrenocortical cell lines","volume":"44","author":"T Wang","year":"2012","journal-title":"Horm Metab Res"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0047-6374(91)90115-G","article-title":"A model for phenotypic switching in adrenocortical cells senescing in\n                    culture","volume":"58","author":"P J Hornsby","year":"1991","journal-title":"Mech Ageing Dev"},{"key":"ref34","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1093\/toxsci\/kfh191","article-title":"Assessment of the effects of chemicals on the expression of ten steroidogenic\n                    genes in the H295R cell line using real-time PCR","volume":"81","author":"K Hilscherova","year":"2004","journal-title":"Toxicol Sci"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"966","DOI":"10.1016\/j.ctrv.2013.03.003","article-title":"Strategies for managing ACTH dependent mineralocorticoid excess induced by\n                    abiraterone","volume":"39","author":"A Pia","year":"2013","journal-title":"Cancer Treat Rev"},{"key":"ref37","doi-asserted-by":"publisher","first-page":"e0196931.","DOI":"10.1371\/journal.pone.0196931","article-title":"CYP11B1 has no role in mitotane action and metabolism in adrenocortical\n                    carcinoma cells","volume":"13","author":"A Germano","year":"2018","journal-title":"PloS 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