{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T20:09:21Z","timestamp":1769112561417,"version":"3.49.0"},"reference-count":44,"publisher":"Georg Thieme Verlag KG","issue":"13\/14","funder":[{"name":"COST FA1103 Endophytes in Biotechnology and Agriculture"},{"DOI":"10.13039\/501100005781","name":"Koneen S\u00e4\u00e4ti\u00f6","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005781","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"crossref","award":["A71142"],"award-info":[{"award-number":["A71142"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Despite the continuing interest in various plant and natural products, only a small portion of the biologically active compounds from nature has been discovered and exploited. In this study, antioxidant and antibacterial properties of aqueous fractions of three endophytic fungi isolated from the roots of 8-year-old Scots pines (Pinus sylvestris) growing on a drained peatland were investigated. The endophytic fungi species were Acephala applanata, Phialocephala fortinii, and Humicolopsis cephalosporioides\/Coniochaeta mutabilis. The bioactivities were examined using hydrogen peroxide scavenging and oxygen radical absorbance capacity tests as well as sensitive Escherichia coli-based biosensors, which produce a luminescent signal in the presence of substances with oxidative or genotoxic properties. In addition, cell models for Parkinson\u02bcs disease, age-related macular degeneration, and osteoarthritis were used to evaluate the potential for\n\t\t\t\t\tpharmaceutical applications. The aqueous extracts of fungi and 19 out of 42 fractions were found to be active in one or more of the tests used. However, no activity was found in the age-related macular degeneration and osteoarthritis cell model tests. Additionally, bioactivity data was connected with metabolites putatively annotated, and out of 330 metabolites, 177 were interesting in view of the bioactivities investigated. A majority of these were peptides and all three fungal species shared a highly similar metabolome. We propose that Scots pine endophytic fungi are a rich source of interesting metabolites, and synergistic effects may cause the bioactivities, as they were found to vary after the fractionation process.<\/jats:p>","DOI":"10.1055\/a-1185-4437","type":"journal-article","created":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T23:23:48Z","timestamp":1591831428000},"page":"1009-1024","source":"Crossref","is-referenced-by-count":13,"title":["Bioactive Properties of the Aqueous Extracts of Endophytic Fungi Associated with Scots Pine (Pinus sylvestris) Roots"],"prefix":"10.1055","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7089-7832","authenticated-orcid":false,"given":"Jenni","family":"Tienaho","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland"},{"name":"Natural Resources Institute Finland (Luke), Production Systems Unit, Biomass Characterization and Properties Group, Espoo, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maarit","family":"Karonen","sequence":"additional","affiliation":[{"name":"Natural Chemistry Research Group, Department of Chemistry, University of Turku, Turku, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riina","family":"Muilu-M\u00e4kel\u00e4","sequence":"additional","affiliation":[{"name":"Natural Resources Institute Finland (Luke), Production Systems Unit, Biomass Characterization and Properties Group, Espoo, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janne","family":"Kaseva","sequence":"additional","affiliation":[{"name":"Natural Resources Institute Finland (Luke), Natural Resources Unit, Applied Statistical Methods Group, Jokioinen, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuria","family":"de Pedro","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Avda. del Conocimiento, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisca","family":"Vicente","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Avda. del Conocimiento, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Genilloud","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Avda. del Conocimiento, Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulla","family":"Aapola","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannu","family":"Uusitalo","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland"},{"name":"Tays Eye Centre, Tampere University Hospital, Tampere, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katriina","family":"Vuolteenaho","sequence":"additional","affiliation":[{"name":"The Immunopharmacology Research Group, Faculty of Medicine and Health Technology, Tampere University and Tampere University Hospital, Tampere, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Franz\u00e9n","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering, Department of Chemistry and Materials Science, Aalto University, Espoo, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristiina","family":"W\u00e4h\u00e4l\u00e4","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Developmental Biology, University of Helsinki, Helsinki, Finland"},{"name":"Department of Chemistry, University of Helsinki, Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matti","family":"Karp","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ville","family":"Santala","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences and Engineering, Tampere University, Tampere, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tytti","family":"Sarjala","sequence":"additional","affiliation":[{"name":"Natural Resources Institute Finland (Luke), Production Systems Unit, Biomass Characterization and Properties Group, Espoo, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2020,6,10]]},"reference":[{"key":"ref1","first-page":"3","volume-title":"An Overview of endophytic Microbes: Endophytism defined","author":"J 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