{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T07:32:40Z","timestamp":1785310360303,"version":"3.55.0"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"08","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Res (Stuttg)"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Macrolides are clinically well-established class of antibiotics. Macrolides\n                    induce cardiotoxicity by blocking ether-a-go-go\u2013related gene\n                    (ERG) potassium channels in cardiac myocytes. The aim of this study was to\n                    compare the effects of erythromycin, clarithromycin and azithromycin on cell\n                    viability and expression of ERG1 gene in H9c2 cells. Cell viability and ERG1\n                    gene expression of H9c2 cells in 3 different concentrations, 1, 10 and\n                    25\u2009\u00b5g\/ml, after 48 and 72\u2009h were determined by\n                    MTT test and Real time-PCR method respectively. After 48\u2009h, the growth\n                    of H9c2 cells treated with erythromycin, clarithromycin and Azithromycin (except\n                    two doses) were inhibited significantly compared to control group\n                    (p&lt;0.05). All three groups of antibiotics showed toxic effects on cells\n                    after 72\u2009h in all concentrations. Azithromycin-inhibiting effects were\n                    significantly higher than two other groups after 72\u2009h of treatment. The\n                    expression of ERG1 gene increased in all three groups of antibiotics by\n                    increasing the concentration and duration of treatment. Azithromycin had the\n                    most pronounced effect on ERG1 expression in 48 and 72\u2009h. This study\n                    indicated that these macrolides affect ERG1 expression due to their potential\n                    cardiac adverse effects. Further investigations are required to understand the\n                    exact mechanism of cardiotoxicity associated with macrolides.<\/jats:p>","DOI":"10.1055\/a-1185-8762","type":"journal-article","created":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T19:22:57Z","timestamp":1592594577000},"page":"341-347","source":"Crossref","is-referenced-by-count":7,"title":["Comparative Study of the Effect of Macrolide Antibiotics\n                    Erythromycin, Clarithromycin, and Azithromycin on the ERG1 Gene Expression in\n                    H9c2 Cardiomyoblast Cells"],"prefix":"10.1055","volume":"70","author":[{"given":"Nima","family":"Hajimirzaei","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Pharmacology, Faculty of Pharmacy and\n                    Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical\n                    Sciences, Yazd, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazila Pour","family":"Khalili","sequence":"additional","affiliation":[{"name":"Center for Cell Pathology Research, Department of Biological Sciences,\n                    Khazar University, Baku, Azerbaijan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Behshad","family":"Boroumand","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Pharmacology, Faculty of Pharmacy and\n                    Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical\n                    Sciences, Yazd, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Safari","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, Shahid Sadoughi\n                    University of Medical Sciences, Yazd, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armin","family":"Pourhosseini","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Pharmacology, Faculty of Pharmacy and\n                    Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical\n                    Sciences, Yazd, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Judi-Chelan","sequence":"additional","affiliation":[{"name":"Department of Maxillofacial surgery, Shahid Rahnemun Hospital, Shahid\n                    Sadoughi University of Medical Sciences, Yazd, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Tavakoli","sequence":"additional","affiliation":[{"name":"Department of Toxicology and Pharmacology, Faculty of Pharmacy and\n                    Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical\n                    Sciences, Yazd, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2020,6,19]]},"container-title":["Drug Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-1185-8762.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T18:41:57Z","timestamp":1596048117000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-1185-8762"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,19]]},"references-count":0,"journal-issue":{"issue":"08","published-online":{"date-parts":[[2020,7,29]]},"published-print":{"date-parts":[[2020,8]]}},"URL":"https:\/\/doi.org\/10.1055\/a-1185-8762","relation":{},"ISSN":["2194-9379","2194-9387"],"issn-type":[{"value":"2194-9379","type":"print"},{"value":"2194-9387","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,19]]}}}