{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T15:49:12Z","timestamp":1776095352453,"version":"3.50.1"},"reference-count":27,"publisher":"Georg Thieme Verlag KG","issue":"16","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Juniperus (Cupressaceae, Pinales) plants are widely distributed in the Qinghai-Tibet Plateau of China. The leaves and twigs of at least 8 Juniperus species (J. pingii, J. pingii var. wilsonii, J. squamata, J. recurva var. coxii, J. saltuaria, J. indica, J. tibetica and J. convallium var. microsperma) have been used as the Tibetan medicine Xuba. At present, it is difficult to distinguish among the original species of Xuba based only on their similar morphological characteristics. However, in our previous studies, 4 Xuba samples from different Juniperus species exhibited significant differences in both anticomplementary activity in vitro and anti-inflammatory effects on acute lung injury in vivo. To identify the effective original species of Xuba reliably, in this study, we developed a sequencing-based DNA molecular technology to distinguish 14 populations of 8\n\t\t\t\t\tJuniperus species collected from Tibet region, using trnS-G, trnD\u200a\u2013\u200aT, and petN-psbM genomic regions to build phylogenetic trees. In addition, their anticomplementary activities were evaluated. The results showed that combined sequence of these 3 genomic regions could identify 8 Juniperus species clearly and clustered individuals of one species but from different locations, whichever phylogenetic tree was constructed. Moreover, the anticomplementary activities of the 8 species were clustered into 2 groups. Among them, J. saltuaria and J. recurva var. coxii, which formed an independent branch apart from the other 6 species in phylogenetic trees, were the most potent (CH50: 0.029\u200a\u2013\u200a0.032\u2009mg\/mL). Consequently, DNA identification of Juniperus using the combined sequence could provide beneficial guidance for further efficacy evaluation and quality control of Xuba.<\/jats:p>","DOI":"10.1055\/a-1194-0471","type":"journal-article","created":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T22:36:46Z","timestamp":1594334206000},"page":"1176-1184","source":"Crossref","is-referenced-by-count":9,"title":["Molecular Identification Based on Chloroplast Sequences and Anti-complementary Activity Comparison of Juniperus Samples from the Qinghai-Tibet Plateau"],"prefix":"10.1055","volume":"86","author":[{"given":"Yi-Ting","family":"Yang","sequence":"first","affiliation":[{"name":"School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, People\u02bcs Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji","family":"De","sequence":"first","affiliation":[{"name":"College of Science, Tibet University, Lhasa, People\u02bcs Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ze-Long","family":"Fu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, People\u02bcs Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xie-Li","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, People\u02bcs Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dao-Feng","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, People\u02bcs Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Xie","sequence":"additional","affiliation":[{"name":"Human Phenome Institute, Fudan University, Shanghai, People\u02bcs Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, People\u02bcs Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2020,7,9]]},"reference":[{"key":"ref1","first-page":"69","volume-title":"Flora of China 4","author":"I A Al-Shehbaz","year":"1999"},{"key":"ref2","first-page":"173","volume-title":"Xin Xiu Jingzhubencao","author":"D S Luo","year":"2004"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1590\/S0102-695X2012005000045","article-title":"Investigation of antioxidant and anti-glycation properties of essential oils from fruits and branchlets of Juniperus oblonga","volume":"22","author":"S A Emami","year":"2012","journal-title":"Rev Bras Farmacogn"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"M364","DOI":"10.1111\/j.1750-3841.2009.01277.x","article-title":"Chemical composition and antimicrobial and antioxidant activities of essential oils and various extracts of Juniperus phoenicea L. 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