{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T08:47:21Z","timestamp":1745916441864},"reference-count":45,"publisher":"Georg Thieme Verlag KG","issue":"06","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Handchir Mikrochir Plast Chir"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Zusammenfassung<\/jats:title><jats:p>Einleitung\u2002Bei Gewebedefekten k\u00f6nnen epidermale und dermale Anteile der Haut verloren gehen. H\u00e4ufig ist eine reduzierte Gewebeelastizit\u00e4t, ggf. mit Narbenkontrakturen, die die Gelenkbeweglichkeit einschr\u00e4nken k\u00f6nnen, die Folge. Artifizielle Kollagenmatrices und humane azellul\u00e4re dermale Matrices (ADM) stellen ein neues Verfahren der kutanen Rekonstruktion vor allem in der Verbrennungsmedizin dar.<\/jats:p><jats:p>Zielsetzung\u2002Ziel dieser Studie war es, die Eignung von ADM als dermalen Ersatz zu untersuchen. Hierbei wurden die zellul\u00e4re Migration und Differenzierung sowie die inflammatorische Reaktion auf verschiedene Matrices in einem etablierten Hautorgankulturmodell ex vivo untersucht.<\/jats:p><jats:p>Material und Methoden\u2002Reste von vitalen, operativ entnommenen Spalthauttransplantaten wurden auf humane ADM (Epiflex), deepidermalisierte humane Dermis (DED) oder artifizielle Kollagen-Elastin Matrix (KEM, Matriderm) transferiert und das epitheliale Resurfacing in einem standardisierten Wundmodell an der Luft-Feuchtigkeitsgrenze untersucht. Um den Einfluss unterschiedlicher dermaler Anteile auf das epitheliale Verhalten zu untersuchen, wurde zudem die Migration auf ADM aus papill\u00e4ren mit retikul\u00e4ren Dermisanteilen verglichen. Die Reepithelialisierung und zellul\u00e4re inflammatorische Reaktion wurden histologisch, immunhistochemisch und biochemisch analysiert.<\/jats:p><jats:p>Ergebnisse und Schlussfolgerung\u2002Die gr\u00f6\u00dfte epitheliale Ausbreitung und Differenzierung fand auf DED (2,54\u2009mm\u2009\u00b1\u20090,43\u2009mm, Mittelwert\u2009\u00b1\u2009SEM) im Vergleich zu ADM (1,32\u2009mm\u2009\u00b1\u20090,44\u2009mm, p\u2009&lt;\u20090,09) oder KEM (0,77\u2009mm\u2009\u00b1\u20090,11\u2009mm, p\u2009&lt;\u20090,02) statt, was unter anderem auf promigratorische Basalmembranreste auf DED zur\u00fcckzuf\u00fchren ist. Die keratinozyt\u00e4re Migration war deutlich gr\u00f6\u00dfer auf papill\u00e4rer ADM im Vergleich zu retikul\u00e4rer ADM. Im Gegensatz zu den biologischen Matrices fand sich in der grobporigen KEM nur eine horizontale Durchwanderung des Gewebes. Die Expression proinflammatorischer Mediatoren unterschied sich je nach Hautdonor und Matrix.<\/jats:p><jats:p>Zusammenfassend ist festzustellen, dass die Struktur und Herkunft der dermalen Matrix von gro\u00dfer Bedeutung f\u00fcr die Reepithelialiserung und inflammatorische zellul\u00e4re Reaktion sind.<\/jats:p>","DOI":"10.1055\/a-1200-1189","type":"journal-article","created":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T23:01:31Z","timestamp":1596150091000},"page":"533-544","source":"Crossref","is-referenced-by-count":2,"title":["Eignung biologischer azellul\u00e4rer dermaler Matrices als Hautersatz"],"prefix":"10.1055","volume":"52","author":[{"given":"Marcin","family":"Specht","sequence":"additional","affiliation":[{"name":"Klinikum Bremen-Mitte gGmbH Plastisc, Reconstructive and Aesthetic Surgery"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sorge","family":"Kelm","sequence":"additional","affiliation":[{"name":"University of Bremen CBIB, Faculty of Biology and Biochemistry"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ursula","family":"Mirastschijski","sequence":"additional","affiliation":[{"name":"University of Bremen CBIB, Faculty of Biology and Biochemistry"},{"name":"Mira-Beau gender esthetics, Berlin"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2020,7,30]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1177\/1534734608323057","article-title":"Postburn scars: an update","volume":"7","author":"M Stella","year":"2008","journal-title":"Int J Low Extrem Wounds"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1111\/j.1524-475X.2012.00820.x","article-title":"Outcome after burns: an observational study on burn scar maturation and predictors for severe scarring","volume":"20","author":"MB van der Wal","year":"2012","journal-title":"Wound Repair Regen"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.burns.2012.06.009","article-title":"Rehabilitation of burn patients: an underestimated socio-economic burden","volume":"39","author":"U Mirastschijski","year":"2013","journal-title":"Burns"},{"key":"ref5","first-page":"215","article-title":"The cost of post-burn scarring","volume":"28","author":"U Mirastschijski","year":"2015","journal-title":"Annals of burns and fire disasters"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.burns.2007.01.018","article-title":"The use of MatriDerm in early excision and simultaneous autologous skin grafting in burns \u2013 a pilot study","volume":"34","author":"H Ryssel","year":"2008","journal-title":"Burns : journal of the International Society for Burn Injuries"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s10561-010-9187-3","article-title":"Epiflex((R)) a new decellularised human skin tissue transplant: manufacture and properties","volume":"12","author":"E Rossner","year":"2011","journal-title":"Cell and tissue banking"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1111\/j.1365-2133.2005.06997.x","article-title":"Epithelial regeneration from bioengineered skin explants in culture","volume":"154","author":"U Mirastschijski","year":"2006","journal-title":"Br J Dermatol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1097\/PRS.0b013e31829fe461","article-title":"Complete horizontal skin cell resurfacing and delayed vertical cell infiltration into porcine reconstructive tissue matrix compared to bovine collagen matrix and human dermis","volume":"132","author":"U Mirastschijski","year":"2013","journal-title":"Plast Reconstr Surg"},{"key":"ref10","first-page":"1","article-title":"Lung surfactant accelerates skin wound healing: a translational study from in vitro and in vivo experiments to a clinical phase I trial","author":"U Mirastschijski","year":"2020","journal-title":"Scientific reports"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1111\/wrr.12704","article-title":"Novel specific human and mouse stromelysin-1 (MMP-3) and stromelysin-2 (MMP-10) antibodies for biochemical and immunohistochemical analyses","volume":"27","author":"U Mirastschijski","year":"2019","journal-title":"Wound Repair Regen"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1038\/s41586-019-1794-y","article-title":"Patch repair of deep wounds by mobilized fascia","volume":"576","author":"D Correa-Gallegos","year":"2019","journal-title":"Nature"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1034\/j.1600-0625.2001.100506.x","article-title":"Topical synthetic inhibitor of matrix metalloproteinases delays epidermal regeneration of human wounds","volume":"10","author":"MS Agren","year":"2001","journal-title":"Exp Dermatol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.yexcr.2004.06.007","article-title":"Matrix metalloproteinase inhibitor GM 6001 attenuates keratinocyte migration, contraction and myofibroblast formation in skin wounds","volume":"299","author":"U Mirastschijski","year":"2004","journal-title":"Exp Cell Res"},{"key":"ref15","first-page":"55","article-title":"Matrix metalloproteinase inhibitor BB-3103 unlike the serine proteinase inhibitor aprotinin abrogates epidermal healing of human skin wounds ex vivo","volume":"118","author":"U Mirastschijski","year":"2002","journal-title":"The Journal of investigative dermatology"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1172\/JCI117351","article-title":"Distinct populations of basal keratinocytes express stromelysin-1 and stromelysin-2 in chronic wounds","volume":"94","author":"UK Saarialho-Kere","year":"1994","journal-title":"The Journal of clinical investigation"},{"key":"ref17","first-page":"20","article-title":"Matrix Metalloproteinase-3 is Key Effector of TNF-alpha-Induced Collagen Degradation in Skin","author":"U Mirastschijski","year":"2019","journal-title":"International journal of molecular sciences"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1046\/j.1523-1747.2000.00135.x","article-title":"Stromelysin-2 is upregulated during normal wound repair and is induced by cytokines","volume":"115","author":"O Rechardt","year":"2000","journal-title":"The Journal of investigative dermatology"},{"key":"ref19","first-page":"193","article-title":"Diverse functions of matrix metalloproteinases during fibrosis","volume":"7","author":"M Giannandrea","year":"2014","journal-title":"Disease models\u2005&\u2005mechanisms"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"e63555","DOI":"10.1371\/journal.pone.0063555","article-title":"Macrophage migration and invasion is regulated by MMP10 expression","volume":"8","author":"MY Murray","year":"2013","journal-title":"PLoS One"},{"key":"ref21","first-page":"401","article-title":"Human keratinocyte migration on type IV collagen. Roles of heparin-binding site and alpha 2 beta 1 integrin","volume":"71","author":"JP Kim","year":"1994","journal-title":"Lab Invest"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"18828","DOI":"10.1074\/jbc.M100381200","article-title":"Reduced expression of the epithelial adhesion ligand laminin 5\u2005in the skin causes intradermal tissue separation","volume":"276","author":"F Spirito","year":"2001","journal-title":"The Journal of biological chemistry"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.yexcr.2004.02.020","article-title":"Fibroblasts contribute to the deposition of laminin 5\u2005in the extracellular matrix","volume":"296","author":"A Elkhal","year":"2004","journal-title":"Exp Cell Res"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1126\/science.277.5323.225","article-title":"Induction of cell migration by matrix metalloprotease-2 cleavage of laminin-5","volume":"277","author":"G Giannelli","year":"1997","journal-title":"Science"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"e2221","DOI":"10.1097\/GOX.0000000000002221","article-title":"Hyaluronic Acid Accelerates Re-epithelialization and Alters Protein Expression in a Human Wound Model","volume":"7","author":"E Nyman","year":"2019","journal-title":"Plastic and reconstructive surgery Global open"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.16342","article-title":"Hyaluronic acid biomaterial for human tissue engineered skin substitutes: Preclinical comparative in vivo study of wound healing","author":"A Sierra-Sanchez","year":"2020","journal-title":"Journal of the European Academy of Dermatology and Venereology : JEADV"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1177\/10454411010120050201","article-title":"Proteolytic events of wound-healing \u2013 coordinated interactions among matrix metalloproteinases (MMPs), integrins, and extracellular matrix molecules","volume":"12","author":"B Steffensen","year":"2001","journal-title":"Crit Rev Oral Biol Med"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1111\/1523-1747.ep12614202","article-title":"Collagenase in wound healing: effect of wound age and type","volume":"99","author":"MS \u00c5gren","year":"1992","journal-title":"The Journal of investigative dermatology"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1053\/hupa.2002.32221","article-title":"Ectopic localization of matrix metalloproteinase-9 in chronic cutaneous wounds","volume":"33","author":"U Mirastschijski","year":"2002","journal-title":"Human pathology"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.ejcb.2014.10.001","article-title":"Tumor necrosis factor-alpha-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo","volume":"94","author":"MS Agren","year":"2015","journal-title":"Eur J Cell Biol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1242\/jcs.103.3.755","article-title":"Integrin expression and differentiation in transformed human epidermal cells is regulated by fibroblasts","volume":"103","author":"P Kaur","year":"1992","journal-title":"Journal of cell science"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/j.0022-202X.2004.23549.x","article-title":"Basement membrane reconstruction in human skin equivalents is regulated by fibroblasts and\/or exogenously activated keratinocytes","volume":"124","author":"A El Ghalbzouri","year":"2005","journal-title":"The Journal of investigative dermatology"},{"key":"ref33","first-page":"1","volume-title":"Anatomy, physiology, histology, and immunohistochemistry of human skin","author":"J Fenner","year":"2016"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.jbiomech.2008.12.004","article-title":"The effect of composition and microstructure on the viscoelastic properties of dermis","volume":"42","author":"M Ventre","year":"2009","journal-title":"J Biomech"},{"key":"ref35","first-page":"327","article-title":"Development of an engraftable skin equivalent based on matriderm with human keratinocytes and fibroblasts","volume":"41","author":"PA Golinski","year":"2009","journal-title":"Handchir Mikrochir Plast Chir"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s00383-011-2990-5","article-title":"Matriderm(R) 1\u2005mm versus Integra(R) Single Layer 1.3\u2005mm for one-step closure of full thickness skin defects: a comparative experimental study in rats","volume":"28","author":"S Bottcher-Haberzeth","year":"2012","journal-title":"Pediatr Surg Int"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1038\/sj.jid.5700701","article-title":"Inflammation in wound repair: molecular and cellular mechanisms","volume":"127","author":"SA Eming","year":"2007","journal-title":"The Journal of investigative dermatology"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"e57955","DOI":"10.1371\/journal.pone.0057955","article-title":"IL-1beta promotes corneal epithelial cell migration by increasing MMP-9 expression through NF-kappaB- and AP-1-dependent pathways","volume":"8","author":"HC Tseng","year":"2013","journal-title":"PLoS One"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.2741\/2277","article-title":"Wound re-epithelialization: modulating keratinocyte migration in wound healing","volume":"12","author":"Raja","year":"2007","journal-title":"Front Biosci"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1152\/physrev.2003.83.3.835","article-title":"Regulation of wound healing by growth factors and cytokines","volume":"83","author":"S Werner","year":"2003","journal-title":"Physiol Rev"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"231","DOI":"10.3892\/etm.2011.402","article-title":"Influence of interleukin-8 (IL-8) and IL-8 receptors on the migration of human keratinocytes, the role of PLC-gamma and potential clinical implications","volume":"3","author":"WG Jiang","year":"2012","journal-title":"Exp Ther Med"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1006\/jsre.2000.5892","article-title":"Bioactive interleukin-8 is expressed in wounds and enhances wound healing","volume":"93","author":"HO Rennekampff","year":"2000","journal-title":"J Surg Res"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1111\/1523-1747.ep12616634","article-title":"Interleukin-8 stimulates calcium transients and promotes epidermal cell proliferation","volume":"99","author":"A Tuschil","year":"1992","journal-title":"The Journal of investigative dermatology"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1046\/j.1524-475x.2000.00216.x","article-title":"Interleukin-8 levels and activity in delayed-healing human thermal wounds","volume":"8","author":"JA Iocono","year":"2000","journal-title":"Wound Repair Regen"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"2530","DOI":"10.2337\/db12-1714","article-title":"High-glucose environment enhanced oxidative stress and increased interleukin-8 secretion from keratinocytes: new insights into impaired diabetic wound healing","volume":"62","author":"CC Lan","year":"2013","journal-title":"Diabetes"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1111\/j.1524-475X.2010.00609.x","article-title":"Biomaterials modulate interleukin-8 and other inflammatory proteins during reepithelialization in cutaneous partial-thickness wounds in pigs","volume":"18","author":"KR Kleinbeck","year":"2010","journal-title":"Wound Repair Regen"}],"container-title":["Handchirurgie \u00b7 Mikrochirurgie \u00b7 Plastische 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