{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:34:26Z","timestamp":1740126866063,"version":"3.37.3"},"reference-count":43,"publisher":"Georg Thieme Verlag KG","issue":"01\/02","funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","award":["#13\/07600-3"],"award-info":[{"award-number":["#13\/07600-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","award":["#14\/50249-8"],"award-info":[{"award-number":["#14\/50249-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","award":["#2019\/04788-8"],"award-info":[{"award-number":["#2019\/04788-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","award":["Finance Dode 001"],"award-info":[{"award-number":["Finance Dode 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fungi are a rich source of bioactive compounds. Fungal cocultivation is a method of potentiating chemical interactions and, consequently, increasing bioactive molecule production. In this study, we evaluated the bactericidal, antiprotozoal, and cathepsin V inhibition activities of extracts from axenic cultures of 6 fungi (Fusarium guttiforme, Pestalotiopsis diospyri, Phoma caricae-papayae, Colletotrichum horii, Phytophthora palmivora, and C. gloeosporioides) that infest tropical fruits and 57\u00a0extracts obtained by their cocultivation. Our results reveal that fungal cocultivation enhances the biological activity of the samples, since all extracts that were active on Gram-positive bacteria, Gram-negative bacteria, Trypanosoma cruzi, and Leishmania infantum were obtained from cocultivation. Bacterial growth is either totally or partially inhibited by 46% of the extracts. Two extracts containing mainly fusaric and 9,10-dehydrofusaric acids were\n\t\t\t\t\tparticularly active. The presence of the fungus F. guttiforme in co-cultures that give rise to extracts with the highest activities against L.\u00a0infantum. An axenic culture gave rise to the most active extract for the inhibition of cathepsin V; however, other coculture extracts also exhibited activity toward this biological target. Therefore, the results of the biological activities indicate that fungal cocultivation increased the biological potential of samples, likely due to the hostile and competitive environment that pushes microorganisms to produce substances important for defense and allows access to metabolic routes then silenced in milder cultivation conditions.<\/jats:p>","DOI":"10.1055\/a-1200-2046","type":"journal-article","created":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T22:36:46Z","timestamp":1594334206000},"page":"187-195","source":"Crossref","is-referenced-by-count":1,"title":["Liquid Fungal Cocultivation as a Strategy to Access Bioactive Metabolites"],"prefix":"10.1055","volume":"87","author":[{"given":"Airton Damasceno","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Federal University of S\u00e3o Carlos (UFSCar), S\u00e3o Carlos, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra Regina Pepe","family":"Ambrozin","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology, Federal University of Alfenas (Unifal-MG), Po\u00e7os de Caldas, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Fl\u00e1via S.","family":"de Camargo","sequence":"additional","affiliation":[{"name":"S\u00e3o Carlos Institute of Physics, University of S\u00e3o Paulo (USP), S\u00e3o Carlos, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felipe De Paula Nogueira","family":"Cruz","sequence":"additional","affiliation":[{"name":"S\u00e3o Carlos Institute of Physics, University of S\u00e3o Paulo (USP), S\u00e3o Carlos, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo Luiz Gomes","family":"Ferreira","sequence":"additional","affiliation":[{"name":"S\u00e3o Carlos Institute of Physics, University of S\u00e3o Paulo (USP), S\u00e3o Carlos, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata","family":"Krogh","sequence":"additional","affiliation":[{"name":"S\u00e3o Carlos Institute of Physics, University of S\u00e3o Paulo (USP), S\u00e3o Carlos, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taynara Lopes","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo (USP), Ribeir\u00e3o Preto, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilana Lopes Baratella da Cunha","family":"Camargo","sequence":"additional","affiliation":[{"name":"S\u00e3o Carlos Institute of Physics, University of S\u00e3o Paulo (USP), S\u00e3o Carlos, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriano Defini","family":"Andricopulo","sequence":"additional","affiliation":[{"name":"S\u00e3o Carlos Institute of Physics, University of S\u00e3o Paulo (USP), S\u00e3o Carlos, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4207-6217","authenticated-orcid":false,"given":"Paulo Cezar","family":"Vieira","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Federal University of S\u00e3o Carlos (UFSCar), S\u00e3o Carlos, SP, Brazil"},{"name":"Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo (USP), Ribeir\u00e3o Preto, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2020,7,9]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1021\/acs.jnatprod.5b01055","article-title":"Natural products as sources of new drugs from 1981 to 2014","volume":"79","author":"D J Newman","year":"2016","journal-title":"J Nat Prod"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bcp.2016.10.010","article-title":"Endophytes as sources of antibiotics","volume":"134","author":"E Martinez-Klimova","year":"2017","journal-title":"Biochem Pharmacol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"3670","DOI":"10.1016\/j.bbagen.2013.02.008","article-title":"Natural products: a continuing source of novel drug leads","volume":"1830","author":"G M Cragg","year":"2013","journal-title":"Biochim Biophys Acta Gen Subj"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1055\/a-0847-1532","article-title":"Brazilian plants: an unexplored source of endophytes as producers of active metabolites","volume":"85","author":"D C Savi","year":"2019","journal-title":"Planta Med"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1016\/j.biotechadv.2014.03.001","article-title":"Metabolite induction via microorganism co-culture: a potential way to enhance chemical diversity for drug discovery","volume":"32","author":"S Bertrand","year":"2014","journal-title":"Biotechnol Adv"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"5373","DOI":"10.1038\/s41598-017-05532-5","article-title":"Molecular inter-kingdom interactions of endophytes isolated from Lychnophora ericoides","volume":"7","author":"A M Caraballo-Rodr\u00edguez","year":"2017","journal-title":"Sci Rep"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.micres.2018.04.005","article-title":"Chemical interaction of endophytic fungi and actinobacteria from Lychnophora ericoides in co-culture","volume":"213","author":"F O Chagas","year":"2018","journal-title":"Microbiol Res"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.3390\/md12021043","article-title":"Co-cultivation \u2013 a powerful emerging tool for enhancing the chemical diversity of microorganisms","volume":"12","author":"A Marmann","year":"2014","journal-title":"Mar Drugs"},{"key":"ref9","first-page":"481","article-title":"Cocultivation \u2013 powerful tool for the production of secondary metabolites","volume":"7","author":"R Vigneshwari","year":"2015","journal-title":"J Chem Pharm Res"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"575","DOI":"10.2217\/fmb.14.25","article-title":"Microbial co-culture: harnessing intermicrobial signaling for the production of novel antimicrobials","volume":"9","author":"S Moody","year":"2014","journal-title":"Future Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"112960","DOI":"10.1016\/j.jpba.2019.112960","article-title":"A new approach for identifying antagonism among fungi species and antifungal activity","volume":"179","author":"A Damasceno Silva","year":"2020","journal-title":"J Pharm Biomed Anal"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"10868","DOI":"10.1038\/srep10868","article-title":"Expanding the chemical space for natural products by Aspergillus-Streptomyces co-cultivation and biotransformation","volume":"5","author":"C Wu","year":"2015","journal-title":"Sci Rep"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"14444","DOI":"10.1039\/c3ra42378f","article-title":"Induction of diverse secondary metabolites in Aspergillus fumigatus by microbial co-culture","volume":"3","author":"M E Rateb","year":"2013","journal-title":"RSC Adv"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1007\/s10600-011-0053-8","article-title":"Aspergicin, a new antibacterial alkaloid produced by mixed fermentation of two marine-derived mangrove epiphytic fungi","volume":"47","author":"F Zhu","year":"2011","journal-title":"Chem Nat Compd"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"4039","DOI":"10.1128\/AEM.62.11.4039-4043.1996","article-title":"Production of fusaric acid by Fusarium species","volume":"62","author":"C W Bacon","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1016\/j.ejmech.2016.11.044","article-title":"Fusaric acid and analogues as Gram-negative bacterial quorum sensing inhibitors","volume":"126","author":"T T Tung","year":"2017","journal-title":"Eur J Med Chem"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"354","DOI":"10.3390\/toxins8120355","article-title":"Fast screening of antibacterial compounds from Fusaria","volume":"8","author":"T E Sondergaard","year":"2016","journal-title":"Toxins (Basel)"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1139\/w00-045","article-title":"Effects of the Fusarium spp. mycotoxins fusaric acid and deoxynivalenol on the growth of Ruminococcus albus and Methanobrevibacter ruminantium","volume":"46","author":"H D May","year":"2000","journal-title":"Can J Microbiol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1111\/j.1365-2672.2007.03581.x","article-title":"Bikaverin and fusaric acid from Fusarium oxysporum show antioomycete activity against Phytophthora infestants","volume":"104","author":"S W Son","year":"2008","journal-title":"J Appl Microbiol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1111\/j.1365-2672.2005.02770.x","article-title":"Growth-inhibiting effects of concentrations of fusaric acid on the growth of Bacillus mojavensis and other biocontrol Bacillus species","volume":"100","author":"C W Bacon","year":"2006","journal-title":"J Appl Microbiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/0031-9422(95)00716-4","article-title":"Fusaric and 9,10-dehydrofusaric acids and their methyl esters from Fusarium nygamai","volume":"41","author":"R Capasso","year":"1996","journal-title":"Phytochemistry"},{"key":"ref22","first-page":"65","article-title":"Identification of phytotoxic metabolites of a new Fusarium sp. inhibiting germination of Striga hermonthica seeds","volume":"42","author":"A E Idris","year":"2003","journal-title":"Phytopathol Mediterr"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1016\/j.micinf.2003.08.009","article-title":"Antimicrobial resistance of Pseudomonas aeruginosa biofilms","volume":"5","author":"E Drenkard","year":"2003","journal-title":"Microbes Infect"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1590\/S1517-83822010000200024","article-title":"Leishmanicidal, trypanocidal, and cytotoxic activities of endophytic fungi associated with bioactive plants in Brazil","volume":"41","author":"L H Rosa","year":"2010","journal-title":"Braz J Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/j.1574-695X.2007.00354.x","article-title":"Biological activities from extracts of endophytic fungi isolated from Viguiera arenaria and Tithonia diversifolia","volume":"52","author":"D O Guimar\u00e3es","year":"2008","journal-title":"FEMS Immunol Med Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/nrd2092","article-title":"Targeting proteases: successes, failures and future prospects","volume":"5","author":"B Turk","year":"2006","journal-title":"Nat Rev Drug Discov"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1002\/hlca.200800333","article-title":"Cathepsin B inhibitory tetraene lactones from the fungus Talaromyces wortmannii","volume":"92","author":"Y Dong","year":"2009","journal-title":"Helv Chim Acta"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1271\/bbb.65.1195","article-title":"Aurantiameid acetate, a selective cathepsin inhibitor, produced by Aspergillus penicilloides","volume":"65","author":"K Isshiki","year":"2001","journal-title":"Biosci Biotechnol Biochem"},{"key":"ref29","first-page":"395","article-title":"Potent inhibitors of cysteine proteases from the marine fungus Microascus longirostris","volume":"49","author":"C M Yu","year":"1995","journal-title":"J Antibiot"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1007\/s00253-011-3834-x","article-title":"Microbial and fungal protease inhibitors \u2013 current and potential applications","volume":"93","author":"J Sabotic","year":"2012","journal-title":"Appl Microbiol Biotechnol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"20104","DOI":"10.1074\/jbc.M001392200","article-title":"Clitocypin, a new type of cysteine proteinase inhibitor form fruit bodies of mushroom Clitocybe nebularis","volume":"275","author":"J Brzin","year":"2000","journal-title":"J Biol Chem"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1016\/j.bmc.2010.12.056","article-title":"Acridone alkaloids as potent inhibitors of cathepsin V","volume":"19","author":"R P Severino","year":"2011","journal-title":"Bioorg Med Chem"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.phytochem.2018.06.009","article-title":"Dimeric chalcones derivatives from Myracrodruon urundeuva act as cathepsin V inhibitors","volume":"154","author":"A LF Sarria","year":"2018","journal-title":"Phytochemistry"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.bjp.2019.04.004","article-title":"New cathepsin V inhibitor from stems of Bowdichia virgilioides","volume":"29","author":"T L Silva","year":"2019","journal-title":"Rev Bras Farmacogn"},{"volume-title":"Performance Standards for antimicrobial Susceptibility Testing. 27th ed. CLSI supplement M100","year":"2017","author":"CLSI","key":"ref35"},{"volume-title":"Methods for Dilution antimicrobial Susceptibility Tests for Bacteria that grow aerobically. Approved standard, 10th Edition. CLSI document M07-A10","year":"2015","author":"CLSI","key":"ref36"},{"key":"ref37","first-page":"27","article-title":"Visceral leishmaniasis: experimental models for drug discovery","volume":"133","author":"S Gupta","year":"2011","journal-title":"Indian J Med Res"},{"volume-title":"Drug Screening for Kinetoplastid Diseases","year":"2009","author":"J R Ioset","key":"ref38"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.mimet.2008.05.026","article-title":"Rapid fluorescent assay for screening drugs on Leishmania amastigotes","volume":"75","author":"O Shimony","year":"2008","journal-title":"J Microbiol Methods"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S1383-5769(99)00020-3","article-title":"A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue","volume":"48","author":"J Mikus","year":"2000","journal-title":"Parasitol Int"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"798","DOI":"10.3389\/fchem.2019.00798","article-title":"Structure-based and molecular modeling studies for the discovery of cyclic imides as reversible cruzain inhibitors with ootent anti-Trypanosoma cruzi activity","volume":"7","author":"R AA Ferreira","year":"2019","journal-title":"Front Chem"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"2377","DOI":"10.1021\/bi982175f","article-title":"Human cathepsin V functional expression, tissue distribution, electrostatic surface, enzymatic characterization and chromosomal localization","volume":"38","author":"D Bromme","year":"1999","journal-title":"Biochem"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1042\/bj2010189","article-title":"L-trans-epoxysuccinyl-leucylamido(4-guanidino)butane (E-64) and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and L","volume":"201","author":"A J Barret","year":"1982","journal-title":"Biochem J"}],"container-title":["Planta Medica"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-1200-2046.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,2,5]],"date-time":"2021-02-05T22:52:22Z","timestamp":1612565542000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-1200-2046"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,9]]},"references-count":43,"journal-issue":{"issue":"01\/02","published-online":{"date-parts":[[2021,2,2]]},"published-print":{"date-parts":[[2021,2]]}},"URL":"https:\/\/doi.org\/10.1055\/a-1200-2046","relation":{},"ISSN":["0032-0943","1439-0221"],"issn-type":[{"type":"print","value":"0032-0943"},{"type":"electronic","value":"1439-0221"}],"subject":[],"published":{"date-parts":[[2020,7,9]]}}}