{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T02:51:11Z","timestamp":1781664671761,"version":"3.54.5"},"reference-count":45,"publisher":"Georg Thieme Verlag KG","issue":"16","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Planta Med"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tapinanthus globiferus is often referred to as an all-purpose herb for the treatment of stroke and epilepsy. The present study investigates the anticonvulsant effect of methanolic leaf extract, active fractions, and lupeol (isolate) of Tapinanthus globiferus in mice as well as the underlying mechanisms. Following phytochemical studies of T. globiferus, preliminary assays were performed to evaluate MLE-induced toxic effect and behavioral changes. The pentylenetetrazol (70\u2009mg\/kg, i.\u200ap.)-induced seizure was evaluated in mice that were pretreated orally with vehicle 10\u2009mL\/kg, MLE (4, 20, or 100\u2009mg\/kg), fractions (F1 to F6), lupeol 10\u2009mg\/kg or diazepam (3\u2009mg\/kg). Methanolic leaf extract preserved neuron viability as well as the relative organ weight, and hematological and biochemical parameters. The behavioral endpoints, neuromuscular coordination, and sensory response parameters revealed a dose-dependent effect of methanolic leaf extract. This extract, active fractions, lupeol, and diazepam potentiated the hypno-sedative effect of the barbiturate and attenuated PTZ-induced acute seizure. This antiseizure effect was completely reversed by flumazenil 2\u2009mg\/kg (benzodiazepine site antagonist). Altogether, the benzodiazepine site-mediated anticonvulsant effects of methanolic leaf extract, active fractions, and lupeol corroborate traditional application of T. globiferus against epilepsy.<\/jats:p>","DOI":"10.1055\/a-1209-1254","type":"journal-article","created":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T22:48:01Z","timestamp":1594853281000},"page":"1204-1215","source":"Crossref","is-referenced-by-count":7,"title":["Involvement of the Benzodiazepine Site in the Anticonvulsant Activity of Tapinanthus globiferus against Pentylenetetrazole-induced Seizures in Mice"],"prefix":"10.1055","volume":"86","author":[{"given":"Christianah A.","family":"Elusiyan","sequence":"additional","affiliation":[{"name":"Drug Research and Production Unit, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Luiza Gon\u00e7alves","family":"Faria","sequence":"additional","affiliation":[{"name":"University center, UniEVANG\u00c9LICA, Cidade Universit\u00e1ria, An\u00e1polis, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ane Emanuelle Queiroga","family":"Mendes","sequence":"additional","affiliation":[{"name":"University center, UniEVANG\u00c9LICA, Cidade Universit\u00e1ria, An\u00e1polis, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irans\u00e9 Oliveira","family":"Silva","sequence":"additional","affiliation":[{"name":"University center, UniEVANG\u00c9LICA, Cidade Universit\u00e1ria, An\u00e1polis, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Luis Rodrigues","family":"Martins","sequence":"additional","affiliation":[{"name":"University center, UniEVANG\u00c9LICA, Cidade Universit\u00e1ria, An\u00e1polis, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Alves","family":"Rosa","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Goi\u00e1s, Goi\u00e2nia, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Rodrigues","family":"Pedrino","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Goi\u00e1s, Goi\u00e2nia, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elson Alves","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Sciences, Institute of Biological Sciences, Federal University of Goi\u00e1s, Goi\u00e2nia, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed Ali","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"National Center for Natural Products Research, University of Mississippi, University, Mississippi, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan K.","family":"Zjawiony","sequence":"additional","affiliation":[{"name":"National Center for Natural Products Research, University of Mississippi, University, Mississippi, United States"},{"name":"Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, University, Mississippi, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7440-7581","authenticated-orcid":false,"given":"James Oluwagbamigbe","family":"Fajemiroye","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Sciences, Institute of Biological Sciences, Federal University of Goi\u00e1s, Goi\u00e2nia, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2020,7,15]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1111\/epi.12605","article-title":"An unknown quantity \u2013 the worldwide prevalence of epilepsy","volume":"55","author":"G S Bell","year":"2014","journal-title":"Epilepsia"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1017\/CBO9780511544873","volume-title":"Neurodegenerative Diseases","author":"M F Beal","year":"2005"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1212\/WNL.0000000000000610","article-title":"The global burden of neurologic diseases","volume":"83","author":"J H Chin","year":"2014","journal-title":"Neurology"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1684\/j.1950-6945.2006.tb00199.x","article-title":"Epilepsy and neurodegenerative diseases in adults: a clinical 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