{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:34:09Z","timestamp":1740126849823,"version":"3.37.3"},"reference-count":13,"publisher":"Georg Thieme Verlag KG","issue":"03","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Klin Monbl Augenheilkd"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n          Background After preparation of a graft for Descemet membrane endothelial keratoplasty (DMEK), the diameter of the graft\u02bcs scroll varies significantly. In particular, narrow scrolls\n\t\t\t\t\tmay complicate DMEK surgery. In this study we investigated how temperature, osmolarity, and deswelling of the donor tissue influence scrolling diameter.<\/jats:p><jats:p>\n          Material and Methods In this study we examined donor corneas that could not be used for transplantation. The diameter of the DMEK donor scrolls was measured after graft preparation\n\t\t\t\t\tby reflected light microscopy under different conditions: (1) The diameter of the roll was measured in Ringer\u02bcs solution at temperatures of 5\u200a\u00b0C, 24\u200a\u00b0C and 35\u200a\u00b0C. (2) To study the effect of\n\t\t\t\t\tdifferent culture conditions we used 6% dextran-containing medium for 10\u2009min, 20\u2009min, 16\u2009h and 24\u2009h, and hyper- and hypoosmolar fluids (5% NaCl or aqua dest) for 5\u2009min each.<\/jats:p><jats:p>\n          Results The median donors age was 62 years. The median endothelial cell density of the donor corneas was 1679 cells\/mm2. The median diameter of the DMEK scroll was 1.35\u2009mm\n\t\t\t\t\tin Ringer\u02bcs solution at 24\u200a\u00b0C (IQR, 1.04\u200a\u2013\u200a1.89). In Ringer\u02bcs solution at different temperatures, the scrolling diameter changed between 0.02\u2009mm and 0.29\u2009mm. The change in osmolarity\n\t\t\t\t\tresulted in a difference of 0.02\u2009mm to 0.46\u2009mm. Deswelling with dextran resulted in a change of scrolling diameter between 0.02\u2009mm and 0.09\u2009mm.<\/jats:p><jats:p>\n          Summary In this experimental case series, the diameter of the DMEK scroll did not change due to changes of temperature, osmolarity or deswelling. Influencing the graft\u02bcs scroll to\n\t\t\t\t\tstandardize and simplify the DMEK surgery remains an interesting goal for future studies.<\/jats:p>","DOI":"10.1055\/a-1227-4748","type":"journal-article","created":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T05:22:54Z","timestamp":1605676974000},"page":"308-312","source":"Crossref","is-referenced-by-count":0,"title":["Scrolling of the Donor Cornea in Dependence of Temperature and Osmolarity for Descemet Membrane Endothelial Keratoplasty"],"prefix":"10.1055","volume":"239","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9746-0078","authenticated-orcid":false,"given":"Markus","family":"Gruber","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Universit\u00e4tsklinikum Freiburg"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Reinhard","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Universit\u00e4tsklinikum Freiburg"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrin","family":"Wacker","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Universit\u00e4tsklinikum Freiburg"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip Christian","family":"Maier","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Augenheilkunde, Universit\u00e4tsklinikum Freiburg"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2020,11,17]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.ophtha.2017.08.015","article-title":"Descemet Membrane Endothelial Keratoplasty: Safety and Outcomes: A Report by the American Academy of Ophthalmology","volume":"125","author":"S X Deng","year":"2018","journal-title":"Ophthalmology"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.ajo.2018.01.018","article-title":"Trends in Corneal Transplantation from 2001 to 2016 in Germany: A Report of the DOG-Section Cornea and its Keratoplasty Registry","volume":"188","author":"E Flockerzi","year":"2018","journal-title":"Am J Ophthalmol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1016\/j.ajo.2013.05.022","article-title":"Potential causes of incomplete visual rehabilitation at 6 months postoperative after descemet membrane endothelial keratoplasty","volume":"156","author":"I Dapena","year":"2013","journal-title":"Am J Ophthalmol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1007\/s00417-015-3248-z","article-title":"Outcomes of Descemet membrane endothelial keratoplasty, Descemet stripping automated endothelial keratoplasty and penetrating keratoplasty from a single centre study","volume":"254","author":"S Heinzelmann","year":"2016","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1097\/ICO.0000000000002120","article-title":"Five-Year Graft Survival and Clinical Outcomes of 500 Consecutive Cases After Descemet Membrane Endothelial Keratoplasty","volume":"39","author":"R S Birbal","year":"2020","journal-title":"Cornea"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.ophtha.2015.09.023","article-title":"Descemet Stripping Endothelial Keratoplasty for Fuchs\u02bc Endothelial Corneal Dystrophy: Five-Year Results of a Prospective Study","volume":"123","author":"K Wacker","year":"2016","journal-title":"Ophthalmology"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1097\/ICU.0000000000000072","article-title":"Optimizing outcomes with Descemet\u02bcs membrane endothelial keratoplasty","volume":"25","author":"F E Kruse","year":"2014","journal-title":"Curr Opin Ophthalmol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.ajo.2018.07.001","article-title":"Elastin Content and Distribution in Endothelial Keratoplasty Tissue Determines Direction of Scrolling","volume":"194","author":"I Mohammed","year":"2018","journal-title":"Am J Ophthalmol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.2147\/OPTH.S143427","article-title":"Effects of temperature and fluid media on the scroll width size of the Descemet\u02bcs membrane endothelial keratoplasty (DMEK) donor graft","volume":"11","author":"E H Koo","year":"2017","journal-title":"Clin Ophthalmol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1016\/j.ophtha.2013.06.038","article-title":"Reproducibility of graft preparations in Descemet\u02bcs membrane endothelial keratoplasty","volume":"120","author":"U Schl\u00f6tzer-Schrehardt","year":"2013","journal-title":"Ophthalmology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1097\/ICO.0b013e3182000e2e","article-title":"A stepwise approach to donor preparation and insertion increases safety and outcome of Descemet membrane endothelial keratoplasty","volume":"30","author":"F E Kruse","year":"2011","journal-title":"Cornea"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.1097\/ICO.0000000000001360","article-title":"\u201cBubble-in-the-Roll\u201d Technique Using the Endoject DMEK Injector: Influence of the Air Bubble on Endothelial Cell Loss","volume":"36","author":"Y Akbaba","year":"2017","journal-title":"Cornea"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1159\/000493571","article-title":"Development of a Donor Tissue Holding Technique for Descemet\u02bcs Membrane Endothelial Keratoplasty Using a 25-Gauge Graft Manipulator","volume":"9","author":"A Kobayashi","year":"2018","journal-title":"Case Rep Ophthalmol"}],"container-title":["Klinische Monatsbl\u00e4tter f\u00fcr Augenheilkunde"],"original-title":[],"language":"de","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1055\/a-1227-4748.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,10]],"date-time":"2023-02-10T14:11:00Z","timestamp":1676038260000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1055\/a-1227-4748"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,17]]},"references-count":13,"journal-issue":{"issue":"03","published-online":{"date-parts":[[2022,3,22]]},"published-print":{"date-parts":[[2022,3]]}},"URL":"https:\/\/doi.org\/10.1055\/a-1227-4748","archive":["Portico","CLOCKSS"],"relation":{},"ISSN":["0023-2165","1439-3999"],"issn-type":[{"type":"print","value":"0023-2165"},{"type":"electronic","value":"1439-3999"}],"subject":[],"published":{"date-parts":[[2020,11,17]]}}}